Oligomerization of indole derivatives with incorporation of thiols

Article abstract of DOI:10.3390/molecules13081846

Two molecules of indole derivative, e.g. indole-5-carboxylic acid, reacted with one molecule of thiol, e.g. 1,2-ethanedithiol, in the presence of trifluoroacetic acid to yield adducts such as 3-[2-(2-amino-5-carboxyphenyl)-1- (2-mercaptoethylthio)ethyl]-1H-indole-5-carboxylic acid. Parallel formation of dimers, such as 2,3-dihydro-1H,1′H-2,3′-biindole-5,5′- dicarboxylic acid and trimers, such as 3,3′-[2-(2-amino-5-carboxyphenyl) ethane-1,1-diyl]bis(1H-indole-5-carboxylic acid) of the indole derivatives was also observed. Reaction of a mixture of indole and indole-5-carboxylic acid with 2-phenylethanethiol proceeded in a regioselective way, affording 3-[2-(2-aminophenyl)-1-(phenethylthio)ethyl]-1H-indole-5-carboxylic acid. An additional product of this reaction was 3-[2-(2-aminophenyl)-1-(phenethylthio) ethyl]-2,3-dihydro-1H,1′H-2,3′-biindole-5′-carboxylic acid, which upon standing in DMSO-d₆ solution gave 3-[2-(2-aminophenyl)-1- (phenethylthio)ethyl]-1H,1′H-2,3′-biindole-5′-carboxylic acid. Structures of all compounds were elucidated by NMR, and a mechanism for their formation was suggested.

Full text of DOI:10.3390/molecules13081846

Molecules 2008, 13, 1846-1863; DOI: 10.3390/molecules13081846
OPEN ACCESS
molecules
ISSN 1420-3049
www.mdpi.org/molecules
Article
Oligomerization of Indole Derivatives with Incorporation of
Thiols
Felikss Mutulis 1, 2 *, Adolf Gogoll 3, Ilze Mutule 1, 2, Sviatlana Yahorava 1, Aleh Yahorau 1,
Edvards Liepinsh 2 and Jarl E.S. Wikberg 1
1
Department of Pharmaceutical Biosciences, Division of Pharmaceutical Pharmacology, Uppsala
Biomedical center, Uppsala University, S-751 24, Uppsala, Sweden; E-mails:
Sviatlana.Yahorava@farmbio.uu.se; Aleh.Yahorau@farmbio.uu.se; Jarl.Wikberg@farmbio.uu.se
2
Latvian Institute of Organic Synthesis, Aizkraukles 21, LV-1006, Riga, Latvia; E-mail:
ilzem@osi.lv; edv@osi.lv
3
Department of Biochemistry and Organic Chemistry, Box 576, Uppsala University, S-751 23,
Uppsala, Sweden; E-mail: Adolf.Gogoll@biorg.uu.se
* Author to whom correspondence should be addressed. E-mail: fmutulis@osi.lv.
Received: 10 June 2008; in revised form: 18 August 2008 / Accepted: 19 August 2008 / Published: 26
August 2008
Abstract: Two molecules of indole derivative, e.g. indole-5-carboxylic acid, reacted with
one molecule of thiol, e.g. 1,2-ethanedithiol, in the presence of trifluoroacetic acid to yield
adducts such as 3-[2-(2-amino-5-carboxyphenyl)-1-(2-mercaptoethylthio)ethyl]-1H-
indole-5-carboxylic acid. Parallel formation of dimers, such as 2,3-dihydro-1H,1'H-2,3'-
biindole-5,5'-dicarboxylic acid and trimers, such as 3,3'-[2-(2-amino-5-carboxy-
phenyl)ethane-1,1-diyl]bis(1H-indole-5-carboxylic acid) of the indole derivatives was also
observed. Reaction of a mixture of indole and indole-5-carboxylic acid with 2-
phenylethanethiol proceeded in a regioselective way, affording 3-[2-(2-aminophenyl)-1-
(phenethylthio)ethyl]-1H-indole-5-carboxylic acid. An additional product of this reaction
was
3-[2-(2-aminophenyl)-1-(phenethylthio)ethyl]-2,3-dihydro-1H,1'H-2,3'-biindole-5'-
carboxylic acid, which upon standing in DMSO-d6 solution gave 3-[2-(2-aminophenyl)-1-
(phenethylthio)ethyl]-1H,1'H-2,3'-biindole-5'-carboxylic acid. Structures of all compounds
were elucidated by NMR, and a mechanism for their formation was suggested.

Molecules 2008, 13
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Keywords: Side reaction, oligomerization of indole derivatives, incorporation of thiols,
regioselectivity, NMR study.
Introduction
Multistage solid phase organic synthesis, which excludes isolation of intermediates steps, is an
attractive method for the preparation of diverse chemical substances [1]. However, a high yield of
desired product at each synthetic stage is particularly important for these methods [2], as various side
reactions, lowering the chemical yield of the individual synthetic steps, are the main limitation of the
method [3]. In order to make the method more efficient, a deeper understanding of these unwanted
processes is desirable.
Scheme 1. Incorporation of thiols. Proposed mechanism and related substances.
In our previous investigation of indole-5-carboxylic acid derivatives attached to carboxylated Wang
polymer [4], LC/MS analysis after the cleavage step using trifluoroacetic acid and 1,2-ethanedithiol
mixture unexpectedly showed the presence of compounds whose molecular mass corresponded to
dimerization of the expected products with addition of 1,2-ethanedithiol.

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Dimers [5-10], trimers [8, 11-17] and tetramers [7, 14, 15, 17] of indole and its derivatives are
described in a number of research articles and patents. It is well documented that indole (1) forms the
indole dimer 2,3-dihydro-1H,1'H-2,3'-biindole [6, 15] (2, Scheme 1) and trimer 2-[2,2-di(1H-indol-3-
yl)ethyl]-aniline [5, 12, 15] (3, Figure 1) under various acidic conditions.
Results and Discussion
To investigate the aforementioned unknown side reaction, in the present study we tried to prepare a
similar compound from indole-5-carboxylic acid itself. Indole-5-carboxylic acid (1a, Scheme 1) was
treated with trifluoroacetic acid and 1,2-ethanedithiol mixture. Indeed, the expected dimerization and
addition reactions furnished the desired product. In addition, according to LC/MS, along with the
dimer and trimer of indole-5-carboxylic acid, a product, whose molecular mass corresponded to the
sum of mass of four molecules of indole-5-carboxylic acid and one molecule 1,2-ethanedithiol was
detected.
Figure 1. Indole trimers.
A detailed NMR investigation of the products was performed using 1H [18], COSY [18, 19], proton
decoupled 13C [18, 20], HETRES [21] and selective long range INEPT [22] experiments, and
evaluation of the resulting NMR data confirmed proposed structures 2a (Scheme 1), 3a (Figure 1), 5a
and 6a (Scheme 1). Moreover, as additional proof of the correctness of the proposed structures, their
corresponding acetylated derivatives 2b (Scheme 1), 3b (Figure 1), 5b and 6b (Scheme 1) were also
prepared and their structures investigated.
1,2-Ethanedithiol is known to be a strong nucleophilic reagent, widely used as the most effective
scavenger of carbonium ions [23]. Incorporation of dithiol could be explained in terms of the
mechanism proposed by Smith [11] and Sundberg [24] for oligomerization of indole (Scheme 1). We
suggest that ethanedithiol reacts with the indole-5-carboxylic acid dimer 4a (Scheme 1) forming an
ethanedithiol adduct 5a. Competitive attachment of the third molecule of indole-5-carboxylic acid
leads to parallel formation of trimer 3a (Figure 1). It is logical to assume that in a way similar to
formation of 5a, the SH group in 5a reacts further with the protonated dimer 4a yielding a symmetric
structure 6a (Scheme 1).
Further, we investigated the influence of substitution pattern in the indole on the course of the
oligomerization. Replacing 1,2-ethanedithiol with 2-phenylethanethiol and varying substituents in the
indole system we obtained products 7a-f analogous to 5a (Scheme 2). Indole-5-carboxylic acid, its
diethylamide [24], indole-6-carboxylic acid, 5-cyanoindole, 5-fluoroindole and 5-chloroindole were

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used. Acetylated derivatives of 2-phenylethanethiol adducts 8a-c (Scheme 2) were prepared to assist in
the spectroscopic structure assignments. Incorporation of thiols was accompanied by formation of
ample quantities of dimers of indoles like 2a, acetylated derivative 2b (Scheme 1) and indole trimers
like 3a, acetylated derivative 3b, and 3c (Figure 1) (according to LC/MS data).
Scheme 2. Incorporation of 2-phenylethanethiol.
It was not possible to obtain an adduct from unsubstituted indole and 1,2-ethanedithiol under
typical reaction conditions. Incorporation of this indole was only possible when it was used in a
mixture with indole-5-carboxylic acid. In addition, it was noted that the reaction proceeded in a
regioselective way yielding 9 (Scheme 3). This reactivity pattern of unsubstituted indole could be
explained on the basis of mechanism shown in Scheme 1. Michael additions of thiols are known to be
facilitated by the electrodeficiency of the participating alkenes [26]. Electron acceptors attached to the
benzene ring of indole should decrease the electron density on the carbon atom being attacked by
thiols in structures like 4a (Scheme 1), thereby promoting the reaction.
Scheme 3. Introduction of unsubstituted indole.

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An additional indole oligomerization product 10, corresponding to the combination of two
molecules of indole, one molecule of indole-5-carboxylic acid and one molecule of 2-phenylethane-
thiol was isolated from the reaction mixture (Scheme 3). Upon standing in DMSO-d6 solution for one
week, the indoline structure in 10 was oxidized quantitatively to yield indole 11.
Yields for the thiol incorporation products reached 30% (Table 1). They were higher if carbonyl
(compounds 5a, 7a-c, 9) or nitrile (7d) groups were attached to the starting indole molecule. Much less
reactive were halogenated indoles (products 7e and 7f). Particular low were the yields for tetramer 6a
and trimer 10.
Table 1. Representative yields for products of incorporation of thiols.
Compound 5a
Yield, %
33
Conclusions
6a
10
1.3
7a
7b
7c
7d
7e
7f
9
19
32
30
15
2
5
19
1
In summary, we have discovered a previously unknown indole oligomerization with incorporation
of thiols, which has potential synthetic utility. The reaction proceeds with parallel formation of indole
3,3´-trimers; both processes require indole derivatives that are free of substitution at both the 2- and 3-
positions. The inclusion of electronegative substituents in benzene ring of indole is another crucial
factor that makes incorporation of thiols possible. Adducts similar to those reported herein might be of
interest as, e.g., potential enzyme inhibitors.
Experimental
General
Reagents were obtained from Aldrich or Fluka. Evaporations of solvents were carried out on a
vacuum rotary evaporator at 30 oC and 20 mbar. TLC was performed using Merck Silica gel 60 F 254
glass plates; flash chromatography was performed using Merck Silica gel (70-230 mesh, pore size
60Å). LC/MS was performed on a Perkin Elmer PE SCIEX API 150EX instrument with a
Turboionspray Ion Source and equipped with a Dr. Maisch Reprosil-Pur C18-AQ, 5 μ, 150 × 3 mm
HPLC column, using a water and acetonitrile gradient with 5 mM ammonium acetate additive. Semi-
preparative HPLC was carried out on a LKB system consisting of a 2150 HPLC Pump, 2152 LC
Controller and 2151 Variable Wavelength Monitor and Vydac RP C18 column (10 × 250 mm, 90 Å,
201HS1010), the eluent being an appropriate concentration of MeCN in water + 0.1% TFA, flow rate
5 mL/min, detection at 280 nm. Freeze-drying was performed at 0.1 milibar on a Lyovac GT2 Freeze-
Dryer (Finn-Aqua) equipped with a Busch 010-112 vacuum pump and a liquid nitrogen trap. Exact
molecular masses were determined on a Micromass Q-Tof2 mass spectrometer equipped with an
1
electrospray ion source. H-NMR spectra were recorded on a Jeol JNM-EX270 or Jeol JNM-EX400,
Bruker DMX-600 spectrometer equipped with a cryoprobe or a Bruker DMX-500 spectrometer.

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Chemical shifts are reported in ppm relative to residual solvent signal [δ (1H) 2.50 ppm, δ (13C) 39.5
ppm]. Two-dimensional spectra recorded included COLOC, HETRES, selective long range INEPT,
13
13
COSY, ROESY, sensitivity-enhanced C-HSQC and C-1H HMBC. ROESY mixing time was 0.1 s.
Pulsed-field gradients were used for all 13C correlation spectra. 13C-HMBC spectra were recorded with
coupling evolution delay for the generation of multiple-bond correlations set to 62.5 ms. ROESY, 13C-
HSQC and 13C-1H HMBC spectra were run with 4096*1024 points data matrix, giving τ2max = 250 ms
for 1H nucleus in acquisition dimension and τ1max = 200 ms for 1H or τ1max = 50 ms for 13C for indirect
dimension; prior to Fourier transform the data matrix was zero-filled twice and multiplication by
shifted sine-bell window function applied. For 1H-13C HMBC the magnitude spectra were calculated.
Procedure A: Preparation of compounds 2a, 3a, 5a and 6a
Indole-5-carboxylic acid (1a, 370 mg, 2.3 mmol) was dissolved in a mixture of trifluoroacetic acid
(5 mL) and 1,2-ethanedithiol (1 mL). After 1 h at room temperature the mixture was evaporated
without heating under water aspirator pump vacuum. Dry ether-hexane (1:1, 50 mL) was added to the
residue and the white precipitate formed was filtered off, washed with 1:1 dry ether-hexane and dried
in vacuo. The raw product was next dissolved in 24 % MeCN in water, centrifuged and the clear
solution applied in several portions onto an HPLC semipreparative column (10 x 250 mm, Vydac RP
C18 90Å Pharmaceutical 201HS1010), eluent - 20 % MeCN in water + 0.1% TFA, flow 5 mL/min,
detection at 280 nm. Eluate fractions, containing putative 2a (RT 5 min), 3a (RT 8 min) and 5a (RT 17
min) were separately pooled. Next the eluent was changed to 80 % MeCN in water + 0.1% TFA and a
peak containing 6a was collected. Freeze drying provided pure 2a (15 mg, 4 %), 5a (156 mg, 33 %)
and partially purified 3a and 6a as powders. The fraction containing 3a was dissolved in 16 % MeCN
in water and applied in several portions onto an HPLC column (4 × 250 mm, Merck Hibar Lichrosorb
RP18, 10μm), eluent - 16 % MeCN in water + 0.1% TFA, flow 2 mL/min, detection at 220 nm. Eluate
fractions containing pure 3a were pooled and lyophilized to give a yellow powder. (yield 15 mg, 4 %).
The fraction containing 6a was purified again on the Vydac column (eluent - 22 % MeCN in water +
0.1% TFA), followed by purification on the Merck column (eluent - 22 % MeCN in water + 0.1%
TFA) to give, after freeze drying, a white powder (2.1 mg, 0.3 %).
Characterization data
2,3-Dihydro-2,3'-biindole-5,5'-dicarboxylic acid (2a): HRMS: M+H+ (C18H15N2O4) 323.1019,
calculated 323.1032; M-H- (C18H13N2O4) 321.0861, calculated 321.0876; Elemental analysis: found,
%: N 8.2, C 64.9, H 4.8; calculated for 3C18H14N2O4·2H2O, %: N 8.38, C 64.67, H 4.62.

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1H-NMR (400 MHz, DMSO-d6, 25°C): δ 3.03 (dd, J = 16.0, 8.8 Hz, 1H, H-3), 3.43 (dd, J = 16.0, 9.2
Hz, 1H, H-3), 5.28 (t, J = 9.2 Hz, 1H, H-2), 6.50 (d, J = 8.0 Hz, 1H, H-7), 7.36 (d, J = 2.0 Hz, 1H, H-
4), 7.53 (d, J = 8.4 Hz, 1H), 7.58 (m, 1H), 7.61 (dd, J = 8.0, 1.6 Hz, 1H, H-6), 7.81 (dd, J = 8.4, 1.6
Hz, 1H), 7.96 (m, 1H), 11.26 (d, J = 1.6 Hz, 1H, H-1), 11.79 (d, J = 2.0 Hz, 1H, H-1’), 12.3 (m, 2H,
2COOH)
3,3'-[2-(2-Amino-5-carboxyphenyl)ethane-1,1-diyl]bis(1H-indole-5-carboxylic acid (3a): HRMS:
M+H+ (C27H22N3O6) 484.1504, calculated 484.1508; M-H- (C27H20N3O6) 482.1331, calculated
482.1352; Elemental analysis: found, %: N 7.7, C 58.5, H 5.4; calculated for C27H21N3O6·4H2O, %: N
7.56, C 58.37, H 5.26.
1H-NMR (270 MHz, DMSO-d6, 25°C): δ 3.22 (m, 2H, 2H-1’’), 4.93 (m, 1H, H-2’’), 6.58 (d, J = 8.6
Hz, 1H, H-3), 7.31 (d, J = 8.6 Hz, 2H, H-7’, H-7’), 7.33, 7.34 (2m, 2H, H-2’, H-2’), 7.35 (m, 1H, H-4),
7.40 (m, 1H, H-6), 7.59 (dd, J = 8.6 Hz, 1.6 Hz, 2H, H-6’, H-6’), 8.08 (d, J = 1.7 Hz, 2H, H-4’, H-4’),
11.14 (d, J = 1.9 Hz, 2H, H-1’, H-1’).
3-(2-(2-Amino-5-carboxyphenyl)-1-(2-mercaptoethylthio)ethyl)-1H-indole)-5-carboxylic acid (5a):
HRMS: M+H+ (C20H21N2O4S2) 417.0957, calculated 417.0943; M-H- (C20H19N2O4S2) 415.0794,
calculated 415.0787; Elemental analysis: found, %: N 6.5, C 57.8, H 5.0; calculated for C20H20N2O4S2,
%: N 6.73, C 57.67, H 4.84.
1H-NMR (400 MHz, DMSO-d6, 25°C): δ 2.34(m, 1H, SH), 2.5 (m, 4H, 2H-1’’’, 2H-2’’’), 3.22 (m, 2H,
2H-1’’), 4.67(m, 1H, H-2’’), 6.61 (d, J = 8.3 Hz, 1H, H-3), 7.39 (d, J = 8.6 Hz, 1H, H-7’), 7.45 (d, J =
2.2 Hz, 1H, H-2’), 7.46 (dd, J = 8.3 Hz, 2.0 Hz, 1H, H-4), 7.53 (d, J = 2.0 Hz, 1H, H-6), 7.71 (dd, J =
13
8.6 Hz, 1.6 Hz, 1H, H-6’), 8.43 (d, J = 1.6 Hz, 1H, H-4’), 11.27 (d, J = 2.2 Hz, 1H, H-1’); C-NMR
(100 MHz, DMSO-d6, 25°C): δ 24.8 (C-2’’’), 35.0 (C-1’’’), 37.2 (C-1’’), 40.0 (C-2’’), 112.0 (C-7’),
116.6 (C-3’), 119.0 (C-5), 121.5 (C-5’), 122.8 (C-4’), 123.1 (C-1), 123.1 (C-6’), 125.9 (C-3a’), 126.0
(C-2’), 129.6 (C-4), 132.8 (C-6), 139.6 (C-7a’), 150.0 (C-2), 168.1 (C-5a), 169.1 (C-5a’).

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3,3'-{1,1'-[Ethane-1,2-diylbis(sulfane-diyl)]bis[2-(2-amino-5-carboxyphenyl)ethane-1,1-diyl]}bis(1H-
indole-5-carboxylic acid) (6a): HRMS: M+H+ (C38H35N4O8S2) 739.1880, calculated 739.1896; M-H-
(C38H33N4O8S2) 737.1733, calculated 737.1740; Elemental analysis: found, %: N 5.3, C 47.9, H 4.2;
calculated for C38H34N4O8S2·2CF3COOH·5H2O, %: N 5.30, C 47.73, H 4.39.
1H-NMR (270 MHz, DMSO-d6, 25°C): δ 2.3 (m, 4H, 2H-1’’’, 2H-1’’’), 3.13 (m, 4H, 2H-1’’, 2H-1’’),
4.55 (m, 2H, H-2’’, H-2’’), 6.55 (d, J = 8.6 Hz, 2H, H-3, H-3), 7.30, 7.31 (2d, J = 2.3 Hz, 2H, H-2’, H-
2’), 7.35 (d, J = 8.6 Hz, 2H, H-7’, H-7’), 7.42-7.64 (m, 2H, H-4, H-4), 7.43-7.66 (m, 2H, H-6, H-6),
7.68 (dd, J = 8.6 Hz, 1.6 Hz, 2H, H-6’, H-6’), 8.38, 8.39 (2d, J = 1.6 Hz, 2H, H-4’, H-4’), 11.18 (d, J =
2.3 Hz, 2H, H-1’, H-1’).
Procedure B: Compounds 2a, 3a, 5a and 6a were also obtained by a modification of Procedure A,
whereby the crude product was dissolved in chloroform and applied onto a glass column filled with
silica gel (pore size 60Å, 70-230 mesh). The column was eluted with chloroform-methanol mixture,
gradually changing its proportions from 20:1 to 1:4, and thereafter the elution was made with pure
methanol. Eluate fractions containing pure 3a and 5a were separately pooled and evaporated. White
crystalline products were obtained. Isolated yield of 3a was 7%, and that of 5a was 25%. (however,
determination of the reaction products by HPLC provided the following yields: 2a: 22%, 3a: 27%, 5a:
28% and 6a: 1.3%).
3a: Elemental analysis. Found, %: N 7.6, C 64.7, H 5.3. Calculated for 3C27H21N3O4·4MeOH, %: N
7.99. C 64.67, H 5.04.
2-(2,2-bis(5-Chloro-1H-indol-3-yl)ethyl)-4-chloroaniline (3c). Compound 3c was obtained from 5-
chloroindole using Procedure A. Yield 63 %. HRMS: M+H+ (C24H19Cl3N3) 454.0637, calculated
454.0644; Elemental analysis: found, %: N 7.17, C 54.81, H 3.45; calculated for
C24H18Cl3N3·CF3COOH, %: N 7.39, C 54.90, H 3.37.

Molecules 2008, 13
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1H-NMR (400 MHz, DMSO-d6, 25°C): δ 3.36 (d, J = 7.9 Hz, 2H, 2H-1’’), 4.89 (t, J = 7.9 Hz, 1H, H-
2’’), 6.78 (m, 1H, H-3), 6.93 (m, 2H, H-4, H-6), 6.99 (dd, J = 8.6 Hz, 2.2 Hz, 2H, H-6’, H-6’), 7.29 (d,
J = 8.6 Hz, 2H, H-7’, H-7’), 7.42 (d, 2H, H-2’, H-2’), 7.49 (d, J = 2.2 Hz, 2H, H-4’, H-4’), 10.99 (d, J
= 2.4 Hz, 2H, H-1’, H-1’); 13C-NMR (67.5 MHz, DMSO-d6, 25°C): δ 32.0 (C-2’’), 35.7 (C-1’’), 113.4
(C-7’, C-7’), 118.2 (C-3’, C-3’), 118.8 (C-4’, C-4’), 120.1 (C-3), 121.2 (C-6’, C-6’), 123.3 (C-5’, C-
5’), 124.9 (C-1, C-2’, C-2’), 126.8 (C-6), 128.2 (C-3a’, C-3a’), 129.8 (C-4), 130.8 (C-5), 135.4 (C-7a’,
C-7a’), 140.0 (C-2).
3-[2-(2-Amino-5-carboxyphenyl)-1-(phenethylthio)ethyl]-1H-indole-5-carboxylic acid (7a). This
compound was prepared according to Procedure A using indole-5-carboxylic acid and 2-
phenylethanethiol as starting materials. Yield 19%; HRMS: M+H+ (C26H25N2O4S) 461.1532,
calculated 461.1535; Elemental analysis: found, %: N 5.82, C 65.44, H 5.31; calculated for
C26H24N2O4S·H2O, %: N 5.85, C 65.25, H 5.48.
1H-NMR (270 MHz, DMSO-d6, 25°C): δ 2.39 and 2.58 (2m, 4H, 2H-1’’’, 2H-2’’’), 3.13-3.32 (m, 2H,
2H-1’’), 4.66 (t, J = 7.6 Hz, 1H, H-2’’), 6.59 (d, J = 8.5 Hz, 1H, H-3), 7.02 (XX´ part of
AA´XX´system, 2H, H-2b, H-6b), 7.08-7.22 (m, 1H, H-4b), 7.08-7.22 (AA´ part of AA´XX´system,
2H, H-3b, H-5b), 7.37 (d, J = 8.6 Hz, 1H, H-7’), 7.45 (m, 2H, H-4, H-6), 7.53 (d, J = 2.3 Hz, 1H, H-
2’), 7.69 (dd, J = 8.6 Hz, 1.6 Hz, 1H, H-6’), 8.43 (d, J = 1.6 Hz, 1H, H-4’), 11.28 (br s, 1H, H-1’).
3-[2-(2-Amino-4-carboxyphenyl)-1-(phenethylthio)ethyl]-1H-indole-6-carboxylic acid (7b). Prepared
according to Procedure A from indole-6-carboxylic acid and 2-phenylethanethiol. Yield 32%; HRMS:
M+H+ (C26H25N2O4S) 461.1548; calculated 461.1535. Elemental analysis: found, %: N 5.26, C 61.88,
H 4.74; calculated for 2C26H24N2O4S·CF3COOH·H2O, %: N 5.32, C 61.59, H 4.88.
1H-NMR (400MHz, DMSO-d6, 25°C): δ 2.42 and 2.52-2.63 (2m, 4H, 2H-1’’’, 2H-2’’’), 3.20-3.32 (m,
2H, 2H-1’’), 4.69 (m, 1H, H-2’’), 6.97 (d, J = 8.0 Hz, 1H, H-H-6), 7.02 (XX´ part of AA´XX´system,
2H, H-2b, H-6b), 7.02 (m, 1H, H-5), 7.09-7.14 (m, 1H, H-4b), 7.16-7.20 (AA´ part of AA´XX´system,

Molecules 2008, 13
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2H, H-3b, H-5b), 7.29 (d, J = 1.6 Hz, 1H, H-3), 7.53 (d, J = 2.4 Hz, 1H, H-2’), 7.58 (dd, J = 8.4, 1.6
Hz, 1H, H-5’), 7.77 (d, J = 8.4 Hz, 1H, H-4’), 7.96 (dd, J = 1.6, 0.8 Hz, 1H, H-7’), 11.26 (d, J = 2.8
Hz, 1H, H-1’).
3-{2-[2-Amino-5-(diethylcarbamoyl)phenyl]-1-(phenethylthio)ethyl}-N,N-diethyl-1H-indole-5-carbox-
amide (7c). Compound 7c was prepared according to Procedure A using indole-5-carboxylic acid
diethylamide [24] and 2-phenylethanethiol as starting materials. Yield 30%; HRMS: M+H+
(C34H43N4O3S) 571.3101; calculated 571.3106; Elemental analysis: found, %: N 9.47, C 69.97, H 7.37;
calculated for 6C34H42N4O2S·CF3COOH, %: N 9.50, C 69.92, H 7.21.
1H-NMR (400 MHz, DMSO-d6, 25°C): δ 0.87 (m, 6H, 2CH3), 1.09 (m, 6H, 2CH3), 2.52-2.63 (m, 4H,
2H-1’’’, 2H-2’’’), 3.02 (m, 4H, 2CH2 NEt), 3.22 (m, 2H, H-1’’), 3.31 (m, 4H, 2CH2 NEt), 4.64(m, 1H,
H-2’’), 6.69 (d, J = 8.0 Hz, 1H, H-3), 6.77 (d, J = 2.0 Hz, 1H, H-6), 6.91 (dd, J = 8.0, 2.0 Hz, 1H, H-
4), 7.00 (XX´ part of AA´XX´system, 2H, H-2b, H-6b), 7.04 (dd, J = 8.4, 1.6 Hz, 1H, H-6’), 7.09-7.13
(m, 1H, 1H-4b), 7.15-7.19 (AA´ part of AA´XX´system, 2H, H-3b, H-5b), 7.33 (d, J = 2.4 Hz, 1H, H-
2’), 7.34 (d, J = 8.4 Hz, 1H, H-7’), 7.71 (m, 1H, 1H-4’), 11.08 (d, J = 2.4 Hz, 1H, H-1’).
3-[2-(2-Amino-5-cyanophenyl)-1-(phenethylthio)ethyl]-1H-indole-5-carbonitrile (7d). 7d was
prepared according to Procedure A from 5-cyanoindole and 2-phenylethanethiol. Yield 15%; HRMS:
M+H+ (C26H23N4S) 423.1674; calculated 423.1643.
1H-NMR (400MHz, DMSO-d6, 25°C): δ 2.43 and 2.52-2.64 (2m, 4H, 2H-1’’’, 2H-2’’’), 3.16 (dd, J =
8.0, 2.0 Hz, 2H, 2H-1’’), 4.70 (t, J = 8.0 Hz, 1H, H-2’’), 6.59 (d, J = 8.8 Hz, 1H, H-3), 7.02 (XX´ part
of AA´XX´system, 2H, H-2b, H-6b), 7.02 (m, 1H), 7.12-7.23 (AA´ part of AA´XX´system, 2H, H-3b,
H-5b), 7.12-7.23 (m, 1H, H-4b), 7.32 (d, J = 2.0 Hz, 1H), 7.36-7.44 (m, 1H), 7.47 (m, 1H), 7.54 (d, J =
2.4 Hz, 1H), 11.48 (br s, 1H, H-1’).

Molecules 2008, 13
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4-Fluoro-2-[2-(5-fluoro-1H-indol-3-yl)-2-(phenethylthio)ethyl]aniline (7e). 7e was prepared according
to Procedure A using 5-fluoroindole and 2-phenylethanethiol as starting materials. Yield 2%; HRMS:
M+H+ (C24H23F2N2S) 409.1533; calculated 409.1550.
4-Chloro-2-[2-(5-chloro-1H-indol-3-yl)-2-(phenethylthio)ethyl]aniline (7f). 7f was prepared according
to Procedure A from 5-chloroindole and 2-phenylethanethiol. Yield 5%; HRMS: M+H+
(C24H23Cl2N2S) 441.0958; calculated 441.0959; Elemental analysis: found, %: N 6.09, C 65.43, H
4.96; calculated for C24H22Cl2N2S, %: N 6.35, C 65.30, H 5.02.
1H-NMR (400MHz, DMSO-d6, 25°C): δ 2.47 and 2.53 (2m, 2H, 2H-1’’’), 2.62 (m, 2H, 2H-2’’’), 3.14
(m, 2H, 2H-1’’), 4.62 (m, 1H, 1H-2’’), 6.56 (d, J = 8.8 Hz, 1H, H-3), 6.83 (dd, J = 8.8, 2.8 Hz, 1H, H-
4), 6.91 (d, J = 2.8 Hz, 1H, H-6), 7.02-7.06 (XX´ part of AA´XX´system, 2H, H-2b, H-6b), 7.02-7.06
(m, 1H, H-6’), 7.12 (m, 1H, H-4b), 7.17-7.22 (AA´ part of AA´XX´system, 2H, H-3b, H-5b), 7.32 (d, J
= 8.4 Hz, 1H, H-7’), 7.41 (d, J = 2.4 Hz, 1H, H-2’), 7.73 (d, J = 2.0 Hz, 1H, H-4’), 11.09 (br s, 1H, H-
1’).
3-[2-(2-Aminophenyl)-1-(phenethylthio)ethyl]-1H-indole-5-carboxylic acid (9). Compound 9 was
prepared according to Procedure A using indole, indole-5-carboxylic acid (equimolar quantities) and
2-phenylethanethiol as starting materials. Yield 19%; HRMS: M+H+ 417.1618. C25H25N2O2S;
calculated 417.1636. Elemental analysis: found, %: N 5.94, C 64.41, H 5.10; calculated for
2C25H24N2O2S· CF3COOH·H2O, %: N 5.81, C 64.71, H 5.33.

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1H-NMR (500 MHz, DMSO-d6, 25°C): δ 2.65 (m, 2H, 2H-2’’’), 3.31, 3.35 (m, 2H, 2H-1’’’), 3.31,
3.35 (m, 2H, 2H-1’’), 4.74 (m, 1H, H-2’’), 6.79 (m, 1H, H-3), 6.96(m, 1H, H-5), 7.03 (m, 1H, H-6),
7.05 (m, 1H, H-4), 7.05 (XX´ part of AA´XX´system, 2H, H-2b, H-6b), 7.14 (m, 1H, H-4b), 7.20 (AA´
part of AA´XX´system, 2H, H-3b, H-5b), 7.40 (d, J = 8.6 Hz, 1H, H-7’), 7.46 (d, J = 2.3 Hz, 1H, H-
13
2’), 7.71 (dd, J = 8.6 Hz, 1.5 Hz, 1H, H-6’), 8.46 (s, 1H, H-4’), 11.28 (d, J = 1.8 Hz, 1H, H-1’). C
NMR (125 MHz, DMSO-d6, 25°C): δ 31.1 (C-1’’’), 34.9 (C-2’’’), 35.6 (C-1’’), 38.7 (C-2’’), 110.6 (C-
7’), 115.2 (C-3’), 118.4 (C-5), 120.2 (C-5’), 121.1 (C-3), 121.4 (C-6’), 121.8 (C-4’), 124.6 (C-2’),
124.7 (C-3a’), 125.2 (C-4b), 126.4 (C-4), 127.4 (C-3b, C-5b), 127.6 (C-2b, C-6b), 129.8 (C-6), 129.9
(C-1), 138.2 (C-7a’), 139.8 (C-1b), 150.0 (C-2), 167.7 (C-5a’).
3-[2-(2-Aminophenyl)-1-(phenethylthio)ethyl]-2,3-dihydro-1H,1'H-2,3'-biindole-5'-carboxylic
acid
(10). This compound was prepared according to Procedure A using indole, indole-5-carboxylic acid
(equimolar quantities) and 2-phenylethanethiol. Yield 1%; HRMS: M+H+ (C33H32N3O2S) 534.2212;
calculated 534.2215.
1H-NMR (500 MHz, DMSO-d6, 25°C): δ 2.23 (m, 2H, 2H-1’’’), 2.30, 2.37 (m, 2H, 2H-2’’’), 2.65 (m,
1H, Ha-1’’), 2.94 (m, 1H, Hb-1’’), 3.54 (m, 1H, H-2’’), 3.98 (m, 1H, H-3’), 5.24 (d, J = 7.6 Hz, 1H, H-
2’), 6.64 (d, J = 7.5 Hz, 1H, H-7’), 6.71 (m, 1H, H-5’), 6.72 (m, 1H, H-3), 6.78 (XX´ part of
AA´XX´system, 2H, H-2, H-6), 6.91(m, 1H, H-5), 6.93 (m, 1H, H-4), 7.05 (m, 1H, H-6), 7.06 (m, 1H,
H-6’), 7.11 (AA´ part of AA´XX´system, 2H, H-3, H-5), 7.14 (m, 1H, H-4), 7.31 (d, J = 7.3 Hz, 1H,
H-4’), 7.35 (m, 1H, H-2’), 7.45 (d, J = 8.6 Hz, 1H, H-7’), 7.74 (dd, J = 8.6 Hz, 1.5 Hz, 1H, H-6’), 8.26
13
(s, 1H, H-4’), 11.35 (m, 1H, H-1’), 12.36 (br, 1H, COOH); C-NMR (125 MHz, DMSO-d6, 25°C):
δ 32.5 (C-1’’’), 34.4 (C-1’’), 34.5 (C-2’’’), 47.1 (C-2’’), 53.1 (C-3’), 57.9 (C-2’), 108.5 (C-7’), 110.6
(C-7’), 117.2 (C-5’), 117.5 (C-5), 117.8 (C-3’), 120.3 (C-5’), 121.6 (C-4’), 121.8 (C-6’), 123.7 (C-4’),
124.4 (C-2’), 124.5 (C-3a’), 125.2 (C-4), 126.9 (C-6), 126.9 (C-6’), 127.3 (C-3, 5), 127.5 (C-2, 6),
130.5 (C-4), 138.6 (C-7a’), 139.6 (C-1), 167.5 (C-COOH).
3-[2-(2-Aminophenyl)-1-(phenethylthio)ethyl]-1H,1'H-2,3'-biindole-5'-carboxylic
acid
(11).
Compound 11 was synthesized from 10 (5 mg) when the latter was dissolved in DMSO-d6 (0.7 mL)
and the solution was allowed to stand in a NMR tube at room temperature for one week.

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1H-NMR (500 MHz, DMSO-d6, 25°C): δ 2.21, 2.30 (m, 4H, 2H-1’’’, 2H-2’’’), 3.39 (m, 1H, Ha-1’’),
3.45 (m, 1H, Hb-1’’), 4.64 (dd, J = 8.9 Hz, 5.9 Hz, 1H, H-2’’), 6.55 (XX´ part of AA´XX´system, 2H,
H-2, H-6), 6.70 (d, J = 7.4 Hz, 1H, H-6), 6.80 (m, 1H, H-2’), 6.94 (m, 1H, H-4), 6.96 (AA´ part of
AA´XX´system, 2H, H-3, H-5), 7.03 (m, 1H, H-5), 7.03 (m, 1H, H-4), 7.07 (m, 1H, H-5’), 7.13 (m,
1H, H-6’), 7.42 (d, J = 8.0 Hz, 1H, H-7’), 7.48 (d, J = 8.6 Hz, 1H, H-7’), 7.79 (dd, J = 8.6 Hz, 1.5 Hz,
1H, H-6’), 8.02 (d, J = 7.9 Hz, 1H, H-4’), 8.36 (s, 1H, H-4’), 11.20 (m, 1H, H-1’), 11.74 (m, 1H, H-
1’), 12.50 (br, 1H, COOH); 13C-NMR (125 MHz, DMSO-d6, 25°C): δ 31.5 (C-1’’’), 35.1 (C-1’’, 2’’’),
39.9 (C-2’’), 107.9 (C-3’), 109.6 (C-3’), 110.7 (C-7’, 7’), 117.7 (C-5’), 119.4 (C-4’), 120.2 (C-6’),
121.4 (C-5’), 121.9 (C-4’), 122.3(C-6’), 125.0 (C-4), 125.2 (C-3a’), 125.3 (C-2’), 125.5 (C-3a’), 126.8
(C-4, 5), 127.2 (C-3, 5), 127.3 (C-2, 6), 129.6 (C-2), 129.7 (C-6), 130.6 (C-1, 2’), 136.5 (C-7a’), 137.9
(C-7a’), 139.4 (C-1), 167.5 (C-COOH).
Procedure C. Preparation of 3,3'-{1,1'-[ethane-1,2-diylbis(sulfanediyl)]bis[2-(2-acetamido-5-
carboxyphenyl)ethane-1,1-diyl]}bis(1H-indole-5-carboxylic acid) (6b). Compound 6a (6.6 mg, 6.24
μmol) was dissolved in DMF (180 μL) and acetic anhydride (30 μL, 318 μmol) was added. The
mixture was allowed to stand for 40 h, diluted with water to 1 mL volume, centrifuged and the clear
solution in several portions applied onto an HPLC semipreparative column (10 x 250 mm, Vydac RP
C18 90Å Pharmaceutical 201HS1010), eluent - 22 % MeCN in water + 0.1% TFA, flow 5 mL/min,
detection at 280 nm. Eluate fractions containing pure putative 6b were pooled and freeze-dried. Yield
ofoff-white powder was 3.4 mg (57%); HRMS: M+H+ (C42H39N4O10S2) 823.2129; calculated
823.2107. M-H- (C42H37N4O10S2) 821.1979; calculated 821.1951; Elemental analysis: found, %: N 6.1,
C 54.3, H 4.6; calculated for 3C42H38N4O10S2·2CF3COOH·9H2O, %: N 5.88. C 54.62, H 4.72.
1H-NMR (500 MHz, DMSO-d6, 25°C): δ 1.88, 1.90 (2s, 6H, H-2c, H-2c), 2.3 – 2.5 (m, 4H, 2H-1’’’,
2H-1’’’), 3.25 - 3.40 (m, 4H, 2H-1’’, 2H-1’’), 4.42, 4.44 (2m, 2H, H-2’’, H-2’’), 7.19 (m, 2H, H-2’, H-
2’), 7.33 (dd, J = 8.5 Hz, 2.7 Hz, 2H, H-7’, H-7’), 7.45 (d, J = 8.6 Hz, 2H, H-3, H-3), 7.64 (m, 2H, H-

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4, H-4), 7.66 (m, 2H, H-6, H-6), 7.68 (m, 2H, H-6’, H-6’), 8.38, 8.39 (2m, 2H, H-4’, H-4’), 9.35, 9.36
(2s, 2H, H-2a, H-2a), 11.14 (m, 2H, H-1’, H-1’), 12.5 (br, 2H, H-5a, H-5a); 13C NMR (125 MHz,
DMSO-d6, 25°C): δ 22.4(C-2c, C-2c), 30.1 (C-1’’’, C-1’’’), 36.3 (C-1’’, C-1’’), 40.5 (C-2’’, C-2’’),
110.6 (C-7’, C-7’), 114.5 (C-3’, C-3’), 120.4 (C-5’, C-5’), 121.6 (C-4’, C-4’), 126.1 (C-5, C-5), 121.8
(C-6’, C-6’), 124.3 (C-3a’, C-3a’), 124.6 (C-2’, C-2’), 126.9 (C-4, C-4), 131.0 (C-6, C-6), 132.3 (C-1,
C-1), 138.6 (C-7a’, C-7a’), 140.0 (C-2, C-2), 166.2 (C-5a, C-5a), 167.8 (C-5a’, C-5a’), 167.8 (C-2b,
C-2b).
The following compounds were similarly prepared by Procedure C:
1-Acetyl-2,3-dihydro-1H,1'H-2,3'-biindole-5,5'-dicarboxylic acid (2b). From 2a. HRMS: M+H+
(C20H17N2O5) 365.1138; calculated 365.1137; M-H- (C20H15N2O5) 363.0961; calculated 363.0981;
Elemental analysis: found, %: N 6.3, C 56.1, H 4.2; calculated for 2C20H16N2O5·CF3COOH·3H2O, %:
N 6.25. C 56.25, H 4.38.
1H-NMR (400 MHz, DMSO-d6, 25°C): δ 2.07 (s, 3H, CH3), 3.01 (d, J = 16.0 Hz, 1H, H-3), 3.79 (dd, J
= 16.0, 10.4 Hz, 1H, H-3), 6.04 (dd, J = 10.0, 2.0 Hz, 1H, H-2), 7.17 (d, J = 2.0 Hz, 1H, H-2’), 7.40 (d,
J = 8.8 Hz, 1H, H-7), 7.69 (dd, J = 8.8, 1.6 Hz, 1H, H-6), 7.77 (d, J = 1.6 Hz, 1H, H-4), 7.88 (dd, J =
8.4, 2.0 Hz, 1H, H-6’), 7.98 (s, 1H, H-4’), 8.18 (m, 1H, H-7’), 11.34 (br s, 1H, H-1’).
3,3'-[2-(2-Acetamido-5-carboxyphenyl)ethane-1,1-diyl]bis(1H-indole-5-carboxylic acid) (3b). From
3a. HRMS: M+H+ (C29H24N3O7) 526.162, calculated 526.1614. M-H- (C29H22N3O7) 524.1467,
calculated 524.1458; Elemental analysis: found, %: N 6.2, C 53.4, H 4.5; calculated for
C29H23N3O4·CF3COOH·3H2O, %: N 6.06. C 53.68, H 4.36.
1H-NMR (400 MHz, DMSO-d6, 25°C): δ 1.88 (s, 3H, CH3), 3.54 (d, J = 7.6 Hz, 2H, 2H-1’’), 4.83 (dd,
J = 7.6, 7.6 Hz, 1H, 1H-2’’), 7.27 (d, J = 2.4 Hz, 2H, H-1’, H-1’), 7.31 (d, J = 8.8 Hz, 2H, H-7’, H-7’),
7.48 (d, J = 8.4 Hz, 1H, H-3), 7.59 (dd, J = 8.8, 1.6 Hz, 2H, H-6’, H-6’), 7.61 (dd, J = 8.4, 2.0 Hz, 1H,
H-4), 7.69 (d, J = 2.0 Hz, 1H, H-6), 8.11 (d, J = 1.6 Hz, 2H, H-4’, H-4’), 9.31 (s, 1H, H-2a), 11.13 (d,
J = 2.4 Hz, 2H, H-1’, H-1’).

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3-[2-(2-Acetamido-5-carboxyphenyl)-1-(2-mercaptoethylthio)ethyl]-1H-indole-5-carboxylic acid (5b).
From 5a. HRMS: M+H+ (C22H23N2O5S2) 459.1037; calculated 459.1048; Elemental analysis: found,
%: N 5.70, C 56.39, H 4.81; calculated for 8C22H22N2O5S2·CF3COOH, %: N 5.92. C 56.52, H 4.72.
1H-NMR (400 MHz, DMSO-d6, 25°C): δ 1.95 (s, 3H, CH3), 2.34(m, 1H, SH), 2.46 and 2.53 (2m, 4H,
2H-1’’’ and 2H-2’’’), 3.34 (m, 1H, 1H-1’’), 3.42 (dd, J = 14.4, 8.4 Hz, 1H, 1H-1’’), 4.49 (m, 1H, 1H-
2’’), 7.31 (d, J = 2.4 Hz, 1H, H-2’), 7.36 (d, J = 8.8 Hz, 1H, H-7’), 7.45 (d, J = 8.4 Hz, 1H, H-3), 7.67
(m, 1H, H-4), 7.69 (dd, J = 8.4, 1.6 Hz, 1H, H-6’), 7.73 (d, J = 1.6 Hz, 1H, H-6), 8.41 (d, J = 1.6 Hz,
1H, H-4’), 9.44 (s, 1H, H-2a), 11.23 (d, J = 2.0 Hz, 1H, H-1’).
3-[2-(2-Acetamido-5-carboxyphenyl)-1-(phenethylthio)ethyl]-1H-indole-5-carboxylic acid (8a). From
7a. HRMS: M+H+ (C28H27N2O5S) 503.1636; calculated 503.1640; Elemental analysis: found, %: N
5.17, C 66.28, H 5.44; calculated for 3C28H26N2O5S·H2O, %: N 5.51. C 66.12, H 5.28.
1H-NMR (270 MHz, DMSO-d6, 25°C): δ 1.92 (s, 3H, CH3), 2.55 and 2.60 (2m, 4H, 2H-1’’’, 2H-2’’’),
3.30-3.49 (m, 2H, 2H-1’’), 4.48 (m, 1H, H-2’’), 7.03 (XX´ part of AA´XX´system, 2H, H-2b, H-6b),
7.08-7.22 (m, 1H, H-4b), 7.08-7.22 (AA´ part of AA´XX´system, 2H, H-3b, H-5b), 7.32 (d, J = 2.3
Hz, 1H, H-2’), 7.37 (d, J = 8.6 Hz, 1H, H-7’), 7.47 (d, J = 8.3 Hz, 1H, H-3), 7.69 (m, 2H, H-4, H-6’),
7.75 (d, J = 1.3 Hz, 1H, H-6), 8.43 (d, J = 1.3 Hz, 1H, H-4’), 9.46 (br s, 1H, H-2a), 11.25 (d, J = 2.3
Hz, 1H, H-1’).
3-[2-(2-Acetamido-4-carboxyphenyl)-1-(phenethylthio)ethyl]-1H-indole-6-carboxylic acid (8b). From
7b. HRMS: M+H+ (C28H27N2O5S) 503.1656; calculated 503.1640; Elemental analysis: found, %: N
5.30, C 65.24, H 5.17; calculated for 4C28H26N2O5S·3H2O, %: N 5.43. C 65.16, H 5.37.

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1H-NMR (270 MHz, DMSO-d6, 25°C): δ 1.97 (s, 3H, CH3), 2.54 and 2.61 (2m, 4H, 2H-1’’’, 2H-2’’’),
3.31-3.51 (m, 2H, 2H-1’’), 4.50 (m, 1H, H-2’’), 7.04 (XX´ part of AA´XX´system, 2H, H-2b, H-6b),
7.09-7.24 (m, 1H, 4b), 7.09-7.24 (AA´ part of AA´XX´system, 2H, H-3b, H-5b), 7.09-7.24 (m, 1H, H-
6), 7.42 (d, J = 2.6Hz, 1H, H-2’), 7.51 (dd, J = 7.9, 2.0 Hz, 1H, H-5), 7.57(dd, J = 8.3, 1.6 Hz, 1H, H-
5’), 7.73 (d, J = 8.3 Hz, 1H, H-4’), 7.84 (d, J = 2.0 Hz, 1H, H-3), 7.96 (d, J = 1.6 Hz, 1H, H-7’), 9.48
(br s, 1H, H-2a), 11.27 (d, J = 2.3 Hz, 1H, H-1’).
3-{2-[2-Acetamido-5-(diethylcarbamoyl)phenyl]-1-(phenethylthio)ethyl}-N,N-diethyl-1H-indole-5-
carboxamide (8c). From 7c. HRMS: M+H+ (C36H45N5O3S) 613.3205; calculated 613.3212; Elemental
analysis: found, %: N 8.63, C 69.01, H 7.03; calculated for 6C36H44N5O3S·CF3COOH H2O, %: N 8.83.
C 68.74, H 7.07.
1H-NMR (400 MHz, DMSO-d6, 25°C): δ 0.74 and 1.08 (2m, 12H, 4CH3), 1.99 (s, 3H, COCH3), 2.51-
2.66 (m, 4H, 2H-1’’’, 2H-2’’’), 3.24-3.44 (m, 10H, 4CH2 NEt, 2H-1’’), 4.46 (dd, J = 8.8, 6.4 Hz, 1H,
H-2’’), 6.89 (d, J = 2.0 Hz, 1H, H-6), 7.02-7.06 (XX´ part of AA´XX´system, 2H, H-2b, H-6b), 7.02-
7.06 (m, 2H, H-4, H-6’), 7.09-7.13 (m, 1H, H-4b), 7.16-7.20 (AA´ part of AA´XX´system, 2H, H-3b,
H-5b), 7.23 (d, J = 2.4 Hz, 1H, H-2’), 7.31 (d, J = 8.0 Hz, 1H, H-3), 7.32 (d, J = 8.4 Hz, 1H, H-7’),
7.68 (m, 1H, H-4’), 9.45 (br s, 1H, H-2a), 11.06 (d, J = 2.4 Hz, 1H, H-1’).
References and Notes
1. Fruechtel, J. S.; Jung, G. Organic chemistry on solid supports. Angew. Chem. Int. Ed. Engl. 1996,
35, 17-42.
2. Brown, R. C. D. Recent developments in solid-phase organic synthesis. J. Chem. Soc., Perkin
Trans. 1 1998, 3293–3320.
3. Crowley, J. I.; Rapoport, H. Solid- Phase Organic Synthesis: Novelty or Fundamental Concept?
Acc. Chem. Res. 1976, 9, 135-144.

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4. Mutulis, F. unpublished data.
5. Keller, K., Polymeric Indoles. Chem. Ber. 1913, 46, 726-733.
6. Wu, Y-H.; Lobeck, W. G., Jr.; Ryan, R. P. Diuretics. 1. 1-Imidoyl-2-(2- and 3-indolyl)indolines.
J. Med. Chem. 1972, 15, 529-534.
7. Bergman, J.; Egestad, B.; Eklund, N. The reaction of indigo with hydrazine. Tetrahedron Lett.
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Sample Availability: Samples of the compounds are available from the authors upon request.
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