Enzymatic synthesis of dipeptides in ionic liquids

Article abstract of DOI:10.2174/157017810790796318

Immobilized protease-catalyzed synthesis of peptides from natural amino acids has been achieved in ionic liquids (ILs). Three ionic liquids BMIM.PF ₆, BMIM.BF₄ and EtPy.BF₄ have been tested; an effective yet simple and practical method has been devised. The product yields are comparable with those seen in molecular solvents. A max yield of 49 % for Boc-Leu-TrpOEt has been obtained. This study shows that the advantages seen in organic solvents can also be achieved in ILs, and use of such medium could provide a viable alternative to organic and aqueous media for bio- catalysis.