Angewandte Chemie - International Edition p. 467 - 471 (2011)
Update date:2022-07-31
Topics:
Avonto, Cristina
Taglialatela-Scafati, Orazio
Pollastro, Federica
Minassi, Alberto
Di-Marzo, Vincenzo
De-Petrocellis, Luciano
Appendino, Giovanni
Speed trap: Cysteamine reacts in DMSO with electrophilic double bonds to afford Michael adducts (see scheme), whereas no trapping reaction takes place in apolar solvents. A simple and quick NMR spectroscopic method to identify Michael acceptors and sort them into reversible and irreversible thiol sinks is validated biologically in a cellular assay sensitive to thiol-trapping agents. DMSO=dimethyl sulfoxide.
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