GLUCOSYLATION OF ISOMERIC HYDROXYBENZOIC ACIDS BY CELL SUSPENSION CULTURES OF MALLOTUS JAPONICUS

Article abstract of DOI:10.1016/0031-9422(90)80120-6

Cultured cells of Mallotus japonicus converted exogenous o-hydroxybenzoic acid into its O-glucoside after a lag period of 8 hr during which time the aglycone was taken up rapidly by the cells, partly excreted and then re-absorbed.The glucosylation of the aglycone into o-O-β-D-glucosylbenzoic acid began almost simultaneously with the induction of glucosyltransferase activity, and ca 78percent of the aglycone administered was transformed into the glucoside in 12 hr.On the other hand, m- and p-hydroxybenzoic acids were glucosylated immediately after administration, the latter yielding both its O-glucoside and glucose ester.Inhibitor experiments suggested the possible participation of either 70S or 80S ribosomes in the glucosylation of isomeric hydroxybenzoic acids.