Tetraethylene glycol-derived spacer for oligonucleotide synthesis

Article abstract of DOI:10.1016/S0040-4039(98)01348-3

3,6,9-Trioxaundecane-1,11-diisocyanate (6) was synthesised from tetraethylene glycol in 5 steps and 48 % overall yield. Spacer 6 was monofunctionalised with the fully protected adenosyl-3'-O-succinate derivative 7 and linked to aminomethyl polystyrene (50 % crosslinked with divinylbenzene) affording a solid support suitable for oligoribonucleotide synthesis (loading: ~20 μmol/g). The HPLC analysis of a crude oligoribonucleotide synthesis and the isolated yield of purified oligomer show that this spacer compares well to hexamethylene diamine.