Angewandte Chemie - International Edition p. 9108 - 9111 (2011)
Update date:2022-08-05
Topics:
Lorenzi, Magali
Puppo, Carine
Lebrun, Regine
Lignon, Sabrina
Roubaud, Valerie
Martinho, Marlene
Mileo, Elisabetta
Tordo, Paul
Marque, Sylvain R. A.
Gontero, Brigitte
Guigliarelli, Bruno
Belle, Valerie
Keeping tabs on tyrosine: A three-component Mannich-type reaction extends the scope of site-directed spin labeling by selectively labeling the unique tyrosine residue of CP12 protein (see picture), as was confirmed by mass spectrometry. EPR spectroscopy of the labeled protein showed a very high mobility of the probe, which remained very mobile after complex formation with GAPDH. Copyright
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