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The strategy we describe evokes the work of Frederick
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ASSOCIATED CONTENT
■
S
* Supporting Information
1
Experimental details, H, 19F, 31P, and 13C NMR spectra, and
MALDI traces. This material is available free of charge via the
AUTHOR INFORMATION
Corresponding Author
■
Notes
The authors declare no competing financial interest.
(32) May, J. F.; Splain, R. A.; Brotschi, C.; Kiessling, L. L. Proc. Natl.
Acad. Sci. U.S.A. 2009, 106, 11851.
(33) Levengood, M. R.; Splain, R. A.; Kiessling, L. L. J. Am. Chem.
Soc. 2011, 133, 12758.
(34) Higher mass peaks also would arise if GlfT2 generates a
mistaken linkage on rare occasion when it first initiates polymerization
from the non-natural acceptor.
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ACKNOWLEDGMENTS
■
We thank Dr. R. A. Splain for the disaccharide precursors of the
hexasaccharide used in the polymerization assays. The NIH is
acknowledged for financial support (R01 AI063596). All
compounds were characterized at the UW-Madison Chemistry
Instrument Center, which is supported by the NSF (CHE-
9208463 and CHE-9974839) and the NIH (NCRR
1S10RR024601).
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