Synthesis and structure of 2-Amino-1-benzyl-4-methylthio-6-oxo-N-phenyl-5- cyano-1,6-dihydropyridin-3-ylcarboxamide

Article abstract of DOI:10.1134/S1070363212030164

Reaction of 3,3-bis(methylthio)-2-cyano-N-phenylacrylamide with N-benzyl-2-cyanoacetamide proceeds regiospecifically to give 2-amino-1-benzyl-4-methylthio-6-oxo-N-phenyl-5-cyano-1,6-dihydropyridin-3- ylcarboxamide. The structure of the latter was studied

Full text of DOI:10.1134/S1070363212030164

ISSN 1070-3632, Russian Journal of General Chemistry, 2012, Vol. 82, No. 3, pp. 461–464. © Pleiades Publishing, Ltd., 2012.
Original Russian Text © V.D. Dyachenko, O.S. Bityukova, 2012, published in Zhurnal Obshchei Khimii, 2012, Vol. 82, No. 3, pp. 468–471.
Synthesis and Structure of 2-Amino-1-benzyl-4-methylthio-6-oxo-
N-phenyl-5-cyano-1,6-dihydropyridin-3-ylcarboxamide
V. D. Dyachenko and O. S. Bityukova
Taras Shevchenko Lugansk National University, Oboronnaya ul. 2, Lugansk, 91011 Ukraine;
е-mail: dvd_lug@online.lg.ua
Received December 30, 2010
Abstract—Reaction of 3,3-bis(methylthio)-2-cyano-N-phenylacrylamide with N-benzyl-2-cyanoacetamide
proceeds regiospecifically to give 2-amino-1-benzyl-4-methylthio-6-oxo-N-phenyl-5-cyano-1,6-dihydropyridin-3-
ylcarboxamide. The structure of the latter was studied by means of XRD analysis.
DOI: 10.1134/S1070363212030164
We have previously shown that the reaction of
cyanoacetanilides with 3,3-bis(methylthio)-2-cyano-N-
arylacrylamides proceeds to form isomeric N,1-diaryl-
1,6-dihydropyridin-3-ylcarboxamides [1], the potential
biologically active compounds [2–7].
the regiospecific intramolecular heterocyclization pro-
ceeds regioselectively involving the nitrile group and
the nitrogen atom of N-benzylcarbamoyl moiety (a).
This can be attributed to the higher nucleophilicity of
the latter, since the lone electron pair of the nitrogen
atom in this case is not conjugated with the benzene
ring.
The present study shows that the reaction of N-
benzyl-2-cyanoacetamide I with 3,3-bis(methylthio)-2-
cyano-N-phenylacrylamide II leads to the formation of
a single reaction product, dihydropyridine III, rather
than a mixture of isomers III and IV, as we expected.
A preliminary evaluation of the possible biological
activity of the compound obtained was carried out
using the PASS Inet program (Prediction of Activity
Spectra for Substances) forecasting the possible types
of biological activity, using the structural formula of a
chemical compound to establish the structure–activity
dependence [8]. By the virtual screening, the
Probably, the first stage of this reaction is the
formation of intermediate A, in which the rotation
around the single bonds followed by the hetero-
cyclization via the a and b ways is possible. However,
b
O
MeS SMe
N
SMe O
O
Ph
N
KOH/DMSO
Ph
CN
H
MeS
N
H
а
Ph
N
H
O
NH
N
CN
Ph
I
II
А
SMe O
SMe O
_MeSH
HCl
NC
O
Ph
NC
O
N
N
Ph
H
III
H
N
NH2
N
NH2
Ph
IV
Ph
III
461

462
DYACHENKO, BITYUKOVA
This is expressed in almost orthogonal orientation of
the N3–C6–O1 amide fragment relative to the hetero-
cycle plane [torsion angle is C1C2C6O1 75.0(2)°]. This
leads to disrupting conjugation between these molecule
fragments resulting in a lengthening of the С2–С6 bond
to 1.506 (2) Å, compared with an average value
(1.46 Å) [9]. The methylthio group is deviated out of
the heterocycle plane and oriented to the cyano group
[torsion angle C13S1C3C4 is –18.95(17)°]. Between
these groups there are shortened intramolecular
contacts H13a···C14 2.56 Å and H13c···C14 2.79 Å (the
sum of van der Waals radii is 2.87 Å [10]), which are
repulsive, as evidenced by a significant increase in the
bond angles S1C3C4 to 126.30(11)° and C3C4C14 to
126.03(14)° compared to with the angles S1C3C2
114.31(11)° and C5C4C14 112.67(14)°, respectively.
The phenyl substituent C16···C21 is oriented perpen-
dicularly to the dihydropyridine ring [torsion angle is
C1N1C15C16 –95.93(16)°], giving rise to much shor-
tened intramolecular contact H2b···H15a 1.88 Å (the sum
of van der Waals radii is 2.32 Å [10]). In this case the
aromatic ring is somewhat turned relative to the
N1–C15 bond [torsion angle N1C15С16С17 is 45.6(2)°].
General view of the structure ІІІ by the X-ray diffraction data.
compound III has the potential anti-asthma and anti-
allergic activities.
The structure of III was confirmed by the X-ray
diffraction analysis (see the figure, Tables 1, 2).
A crystal of compound III is a solvate involving
DMF and ethanol. There is one solvent molecule per
one molecule of dihydropyridine III. The position of
the solvent occupies either DMF or ethanol, with a
0.75:0.25 probability.
A coplanarity of the amide N3–C6–O1 fragment and
phenyl ring C7···C12 [torsion angle C6N3C7C8 is 6.3(3)°]
causes the formation of an intramolecular hydrogen
bond С8–H8···O1 (H···O 2.34 Å, CHO 120°).
The presence of six substituents in the
dihydropyridine ring leads to a significant steric strain.
Table 1. Bond lengths (Å) in the structure ІІІ
In the crystal, the molecules form centrosymmetric
dimers connected by the intermolecular hydrogen
N3–H3···O2' bond [2 – x, –y, –z] (H···O 2.16 Å, NHO
157°) and stacking interactions between the dihydro-
pyridine fragments (the ring center is located at a
distance of 3.49 Å from the C4 atom of the neighboring
molecules in the crystal). These dimers are linked into
the chains along the crystallographic direction (100) by
the hydrogen bonds N2–H2b···O1' [x, 0.5 – y, –0.5 + z]
(H···O 2.16 Å, NHO 159°). Also, a solvent molecule is
bound with compound III molecule via the hydrogen
bond N2–H2a···O(3') [x, 0.5 –y, –0.5 + z] (H···O 1.97 Å,
NHO 158°) (DMF molecule), N2–H2a···O4' [x, 0.5 – y,
–0.5 + z] (H···O 2.05 Å, NHO 146°) (ethanol molecule).
Bond lenght,
(Å)
Bond lenght,
(Å)
Bond
Bond
S1–C3
S1–C13
O1–C6
O2–C5
N1–C1
N1–C5
N1–C15
N2–C1
N3–C6
N3–C7
N4–C14
C1–C2
C2–C3
C2–C6
C3–C4
C4–C14
C4–C5
C7–C8
1.7568(15)
1.777(2)
C7–C12
C8–C9
1.389(2)
1.387(2)
1.370(3)
1.370(3)
1.387(2)
1.508(2)
1.370(2)
1.378(2)
1.382(3)
1.361(3)
1.356(3)
1.388(3)
1.201(4)
1.315(3)
1.453(3)
1.479(3)
1.432(5)
1.536(5)
1.2196(17)
1.2319(18)
1.3728(18)
1.4080(19)
1.4780(18)
1.3293(19)
1.3474(18)
1.4113(18)
1.143(2)
C9–C10
C10–C11
C11–C12
C15–C16
C16–C17
C16–C21
C17–C18
C18–C19
C19–C20
C20–C21
O3–C22
N5–C22
N5–C23
N5–C24
O4–C25
C25–C26
1.401(2)
EXPERIMENTAL
1.398(2)
1.506(2)
The melting point of compound III was determined
1
1.390(2)
on a Koeffler block. The H NMR spectrum was
1.425(2)
recorded on
a Varian Mercury-500 instrument
1.430(2)
(499.9601 MHz) in DMSO-d6 solution (internal
reference TMS). The mass spectrum was registered on
1.382(2)
RUSSIAN JOURNAL OF GENERAL CHEMISTRY Vol. 82 No. 3 2012

SYNTHESIS AND STRUCTURE OF 2-AMINO-1-BENZYL-4-METHYLTHIO-6-OXO-...
463
geometrically and refined in a rider model with Uiso
Table 2. Bond angles (deg) in the structure ІІІ
=
nUeq of the carrier atom (n = 1.5 for the CH3- and OH-
groups and n = 1.2 for the other hydrogen atoms). The
structure was refined with respect to F2 by a full-
matrix least-square method using anisotropic approxi-
mation for non-hydrogen atoms to wR2 0.137 for 7367
reflections [R1 0.053 for 4083 reflections with F >
4σ (F), S 0.97]. In refining, on the bond lengths and
1,3-distances in the disordered solvent molecules the
limits were imposed in accordance with the average
values [10] with an accuracy of 0.005 Å. The bond
lengths and angles are given in Tables 1 and 2,
respectively.
Bond angle
C3S1C13
C1N1C5
C1N1C15
C5N1C15
C6N3C7
N2C1N1
N2C1C2
N1C1C2
C3C2C1
C3C2C6
C1C2C6
C4C3C2
C4C3S1
C2C3S1
C3C4C14
C3C4C5
C14C4C5
O2C5N1
O2C5C4
N1C5C4
ω
Bond angle
C8C7C12
C8C7N3
C12C7N3
C7C8C9
ω
109.67(9)
122.30(12)
122.29(12)
115.40(12)
129.50(13)
118.60(13)
121.79(13)
119.61(13)
120.53(13)
120.74(13)
118.68(12)
119.37(13)
126.30(11)
114.31(11)
126.03(14)
121.24(13)
112.67(14)
118.72(14)
124.37(14)
116.91(13)
119.53(14)
124.44(14)
116.03(14)
119.17(16)
121.34(17)
119.52(16)
120.24(17)
120.18(16)
175.3(2)
C10C9C8
C11C10C9
C10C11C12
C11C12C7
N4C14C4
N1C15C16
C17C16C21
C17C16C15
C21C16C15
C16C17C18
C19C18C17
C20C19C18
C19C20C21
C16C21C20
C22N5C23
C22N5C24
113.12(12)
118.15(16)
122.46(14)
119.37(15)
121.18(18)
120.1(2)
2-Amino-1-benzyl-4-methylthio-6-oxo-N-phenyl-
5-cyano-1,6-dihydropyridin-3-ylcarboxamide (ІІІ).
To a suspension of 0.56 g (10 mmol) of KOH in 10 ml
of DMSO was added 10 mmol of N-benzyl-2-
cyanoacetamide I. The mixture was stirred for 15 min.
After adding 10 mmol of 3,3-bis(methylthio)-2-cyano-
N-phenylacrylamide II, the mixture was stirred for 1 h,
heated at 80°C for 15 min, left standing for 2 h, poured
into cold water, and acidified with an equimolar
amount of 30% aqueous HCl. The resulting precipitate
was filtered off and washed with water. The crystals of
compound III were grown from a DMF–EtOH
mixture. Yield 2.88 g (74%), mp 235–240°С. IR
spectrum, ν, cm–1: 3343, 3450 (NH2), 2209 (CN),
119.71(18)
120.5(2)
120.3(2)
126.7(3)
116.2(3)
O1C6N3
O1C6C2
N3C6C2
124.37(14)
122.34(13)
113.27(12)
C23N5C24
O3C22N5
O4C25C26
116.5(3)
123.6(4)
109.9(5)
1
1650, 1673 (CO). Н NMR spectrum, δ, ppm: 2.55 s
(3Н, SCH3), 5.53 s (2Н, CH2), 7.11 t (1H, НAr, J 7.2 Hz),
7.23 d (2H, НAr, J 8.0 Hz), 7.31–7.39 m (5H, НAr),
7.67 br.s (2H, NH2), 7.69 d (2H, НAr, J 8.5 Hz), 10.45
br.s (1H, NH). Mass spectrum (EI, 70 eV), m/z (Irel,
%): 390 (0.6) [M]+, 298 (0.9) [M – PhNH2]+, 271
(18.5) [M – PhNHCO]+, 167 (1.7), 119 (20.2) [Ph-
N=C=O]+, 91 (100) [PhCH2]+. Found, %: С 64.81; H
4.77; N 14.15. C21H18N4O2S. Calculated, %: C 64.60;
H 4.65; N 14.35.
a MX-1321 instrument (70 eV) with the direct
injection of the sample into the ion source. The
reaction progress and the compound individuality were
monitored by TLC using Silufol UV-254 plates,
eluting with an acetone–hexane mixture (3:5) and
detecting with iodine vapors and UV irradiation.
The crystals of compound III are monoclinic,
C21H18N4O2S·0.75C3H7NO·0.25C2H5OН, at 298 K: a
12.8036(3), b 16.9563(4), c 10.5658(3) Å; β 101.387(3)°,
V 2248.70(10) Å3, M 456.54, Z 4, space group P21/c,
dcalc 1.349 g cm–3, μ(MoKα) 0.18 mm–1, F(000) 962.
The unit cell parameters and intensities of 14186 ref-
lections (7367 independent, Rint 0.019) were measured
on a Xcalibur 3 automatic four-circle diffractometer
(MoKα, graphite monochromator, CCD detector, ω-
scanning, 2θmax 65.06°).
REFERENCES
1. Dyachenko, V.D., Bityukova, O.S., Dyachenko, O.D.,
and Shishkin, O.V., Zh. Obshch. Khim., 2011, vol. 81,
no. 5, p. 857.
2. Hirokawa, Y., Fujiwara, I., Suzuki, K., Harada, H.,
Yoshikawa, T., Yoshida, N., and Kato, S., J. Med.
Chem., 2003, vol. 46, no. 5, p. 702.
3. Onnis, V., Cocco, M.T., Lilliu, V., and Congiu, C.,
Bioorg. Med. Chem., 2008, vol. 16, no. 5, p. 2367.
The structure was solved by the direct method
using a SHELX-97 software package [11]. The
positions of the hydrogen atoms were calculated
4. Mitchell, W.L., Giblin, G.M.P., Naylor, A., Eather-
ton, A.J., Slingsby, B.P., Rawlings, A.D., Jandu, K.S.,
RUSSIAN JOURNAL OF GENERAL CHEMISTRY Vol. 82 No. 3 2012

464
DYACHENKO, BITYUKOVA
Haslam, C.P., Brown, A.J., Goldsmith, P., Clayton, N.M.,
and Sadeghi-Aliabadi, H., Eur. J. Med. Chem., 2009,
vol. 44, no. 8, p. 3253.
7. Pietrangelo, T., Giampietro, L., De Filippis, B., La
Rovere, R., Fulle, S., and Amoroso, R., Europ. J. Med.
Chem., 2010, vol. 45, no. 11, p. 4928.
8. http://195.178.207.233/PASS.
9. Burgi, H.-B. and Dunitz, J.D., Structure Correlation,
Weinheim: VCH, 1994, vol. 2, p. 741.
Wilson, A.W., Chessell, I.P., Green, R.H., Whitting-
ton, A.R., and Wall, I.D., Bioorg. Med. Chem. Lett.,
2009, vol. 19, no. 1, p. 259.
5. Huang, C.Q., Baker, T., Schwarz, D., Fan, J., Heise, C.E.,
Zhang, M., Goodfellow, V.S., Markison, S., Gogas, K.R.,
Chen, T., Wang, X.-C., and Zhu, Y.-F., Bioorg. Med.
Chem. Lett., 2005, vol. 15, nos. 15–16, p. 3701.
10. Zefirov, Yu.V. and Zorkii, P.M., Usp. Khim., 1989,
vol. 58, no. 5, p. 713.
6. Fassihi, A., Azadpour, Z., Delbari, N., Saghaie, L.,
Memarian, H.R., Sabet, R., Alborzi, A., Miri, R.,
Pourabbas, B., Mardaneh, J., Mousavi, P., Moeinifard, B.,
11. Sheldrick, G., Acta Cryst. (A), 2008, vol. 64, p. 112.
RUSSIAN JOURNAL OF GENERAL CHEMISTRY Vol. 82 No. 3 2012