Journal of the American Chemical Society p. 156 - 170 (2017)
Update date:2022-08-02
Topics:
Zhang, Kehui
Sun, Wei
Huang, Linong
Zhu, Kaiyuan
Pei, Fen
Zhu, Longchao
Wang, Qian
Lu, Yingying
Zhang, Hongmin
Jin, Hongwei
Zhang, Li-He
Zhang, Liangren
Yue, Jianbo
Cyclic adenosine diphosphoribose (cADPR), an endogenous nucleotide derived from nicotinamide adenine dinucleotide (NAD+), mobilizes Ca2+ release from endoplasmic reticulum (ER) via ryanodine receptors (RyRs), yet the bridging protein(s) between cADPR and RyRs remain(s) unknown. Here we synthesized a novel photoaffinity labeling (PAL) cADPR agonist, PAL-cIDPRE, and subsequently applied it to purify its binding proteins in human Jurkat T cells. We identified glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as one of the cADPR binding protein(s), characterized the binding affinity between cADPR and GAPDH in vitro by surface plasmon resonance (SPR) assay, and mapped cADPR's binding sites in GAPDH. We further demonstrated that cADPR induces the transient interaction between GAPDH and RyRs in vivo and that GAPDH knockdown abolished cADPR-induced Ca2+ release. However, GAPDH did not catalyze cADPR into any other known or novel compound(s). In summary, our data clearly indicate that GAPDH is the long-sought-after cADPR binding protein and is required for cADPR-mediated Ca2+ mobilization from ER via RyRs.
View MoreTAIXING BEST NEW MATERIALS CO., LTD
Contact:0523-87998158;
Address:No.18 Zhonggang Road,Taixing City ,Jiangsu , China
shijiazhuang baisheng chem co.; ltd
Contact:86-0311-80790826
Address:shijiazhuang hebei
Shanghai Pengkai Chemical Co.,Ltd
Contact:+86-21-60526671
Address:No.4226 Duzhuang Rd Minhang District Shanghai China
HANGZHOU TOYOND BIOTECH CO., LTD
Contact:+86-571-86965177
Address:No. 189, Fengqi East Road, Hangzhou, China
Nanjing Qirui Material Co., Ltd.
Contact:+86-25-52320053
Address:F4-5, #17 Building, Chuang Yi Yuan, No.6 Guanghua East Street, Nanjing, 210007 P.R.China
Doi:10.1021/jm00098a014
(1992)Doi:10.1080/00032719808006493
(1998)Doi:10.1007/BF00905191
()Doi:10.1002/adsc.201200286
(2012)Doi:10.1021/acs.orglett.5b02703
(2015)Doi:10.1039/c3cc41176a
(2013)