A genetically encoded 19F NMR probe for tyrosine phosphorylation

Article abstract of DOI:10.1002/anie.201300463

Simple and selective: Tyrosine phosphorylation is a pivotal post-translational modification which regulates the enzymatic activity, protein conformation, and protein-protein interactions. The highly efficient genetic incorporation of 3,5-difluorotyrosine (F2Y) in E. coli and the use of F2Y as a ¹⁹F NMR probe for the tyrosine phosphorylation are reported (see picture).