Site-specific incorporation of non-natural residues into peptides: Effect of residue structure on suppression and translation efficiencies

Article abstract of DOI:10.1016/S0040-4020(01)81776-2

A systematic survey of the structural requirements for biosynthetic incorporation of non-natural residues into a polypeptide is presented. Relative translation efficiencies for a series of 12 semi-synthetic acylated suppressor tRNAs ranged from 0 to 91% depending on the structure of the residue incorporated.