Combined directed metalation-suzuki-miyaura cross coupling strategies. Synthesis of isomeric chromenopyridinones and related annulated analogues

Article abstract of DOI:10.3987/COM-12-S(N)131

A general strategy encompassing a Directed ortho Metalation (DoM) - Suzuki-Miyaura cross coupling and Directed remote Metalation (DreM) sequence for the synthesis of 5H-chromeno[4,3-c]pyridin-5-ones 5a-j, 5H-chromeno[3,4-b] pyridin-5-ones 6a-j, 5H-chromeno[3,4-c]pyridin-5-ones 13a-d, 12H-benzo[7,8]chromenopyridin-12-ones 16a-c, 17a-c, and pyrido[3',4':4,5] pyrano[2,3-e]indazol-5(1H)-one analogues 18, 28 is reported. Thus, using the powerful directed metalation group properties of aryl O-carbamates 9a-h, 14a-c metalation-boronation followed by Suzuki-Miyaura coupling with 3-bromopyridine affords a variety of azabiaryls 7a-i, 8a-b, 12a-c, 15a-c which, upon DreM reaction leads to several series of chromenopyridinones 5a-j, 6a-j, 13a-d and pyridonaphthopyrones 16a-c, 17a-c. The synthesis of an unusual pyridopyranoindazolone 18 is also described.

Full text of DOI:10.3987/COM-12-S(N)131

HETEROCYCLES, Vol. 86, No. 2, 2012, pp. -. © 2012 The Japan Institute of Heterocyclic Chemistry
Received, 8th September, 2012, Accepted, 8th November, 2012, Published online, 22nd November, 2012
DOI: 10.3987/COM-12-S(N)121
SYNTHESIS OF A LIBRARY OF 1,5,2-DITHIAZEPINE 1,1-DIOXIDES.
PART 1: A ONE-POT SULFONYLATION/THIA-MICHAEL PROTOCOL
Qin Zang,a Aihua Zhou,a Salim Javed,a,b Pradip K. Maity,a Chris A.
Knudtson,a Danse Bi,a Jared J. Hastings,a Fatima Z. Basha,b and Paul R.
Hansona,*
aDepartment of Chemistry, University of Kansas, 1251 Wescoe Hall Drive,
Lawrence, KS 66045-7582 and the University of Kansas Center for Chemical
Methodologies and Library Development (KU-CMLD), 2034 Becker Drive,
Delbert M. Shankel Structural Biology Center, Lawrence, Kansas 66047-3761,
U.S.A.
bH. E. J. Research Institute of Chemistry, International Center for Chemical and
Biological Sciences, University of Karachi, Pakistan
*Fax: (+1) 785-864-5396, e-mail: phanson@ku.edu
Abstract – A novel one-pot sulfonylation/intramolecular thia-Michael protocol is
reported for the synthesis of 1,5,2-dithiazepine 1,1-dioxides. Sulfonylation
between cysteine ethyl ester/cysteamine and 2-chloroethanesulfonyl chloride,
followed by in situ intramolecular thia-Michael addition, was achieved and
afforded the titled 1,5,2-dithiazepine-1,1-dioxide scaffolds. Diversification was
demonstrated for future library synthesis.
Heterocyles have a storied history in drug development due to their diverse pharmacological properties,
which when coupled with advances in high-throughput screening and the need for new pharmaceutical
leads, drive discovery efforts for their efficient synthesis.1 Hetero Michael reactions aspire to this goal,
and have been broadly used in the synthesis of azacycles,2 oxacycles,3,4 thiacycles,5 and bis-heterocycles,6
all of which can be characterized as versatile pathways that are mild, catalytic, and adhere to atom and
step economy. Vinyl sulfones are one particular class of Michael acceptors that have been well
documented in the literature,7 however, their vinyl sulfonamide counterparts have assumed a far less
prominent role. To this end, we have utilized the hetero-Michael addition on vinyl sulfonamides to
synthesize a series of sultams (cyclic sulfonamides, Scheme 1).8
These efforts include a "click, click,
Dedicated to Professor Ei-ichi Negishi on the occasion of his 77th birthday.

cyclize" strategy in the synthesis of 1,4,5-oxathiazepane 4,4-dioxides utilizing an intramolecular
oxa-Michael pathway,8a,b an automated synthesis of 1,2,5-thiadiazepane 1,1-dioxide via an aza-Michael
pathway,8c a double aza-Michael protocol in the synthesis of 1,2,5-thiadiazepane 1,1-dioxide,8d.e and a
Heck-aza-Michael strategy in the synthesis of isoindoline/tetrahydroisoquinoline-based tricyclic
sultams.8f,g Taken collectively, use of "click" sulfonylation,9 "click" sulfonamide alkylation and
subsequent Michael additions greatly simplify sultam construction. In this context, we herein report a
one-pot sulfonylation/intramolecular thia-Michael protocol, which combines the "click" sulfonylation and
the cyclization steps in one pot, to readily assemble the titled sultam scaffolds.
herein reported work
one-pot sulfonylation/intramolecular thia-Michael reaction
Bis-Electrophilic
O
O
Synthon
S
O
O
O
O
Cl
Cl
O
O
A
S
S
1
S
Cl
NH
sulfonylation
thia-Michael
N
R1
B
X
NH2
HS
NH2
R1
2a R1 = H
2b R1 = CO2Et
S
S
3a R1 = H, 32%
3b R1 = CO2Et, 41%
R1
Bis-Nucleophilic
Synthons
previous results related to "click, click, cyclize" strategy
O
O
O
R2
O
O
intramolecular
oxa-Michael
S
R2
S
N
N
N
ref 7a,b
TBSO
R1
R2
R1
O
O
O
O
intermolecular
aza-Michael
S
R2
R1
S
N
2."Click"
R2Br
R1
ref 7c
O
O
HO2C
O
N
S
O
R3
Cl
Cl
O
O
R2
O
double
S
R2
NH2
CO2Me
1. "Click"
S
aza-Michael
N
N
R1
CO2Me
ref 7d,e
CO2Me
N
R1
O
S
O
O
O
S
R2
N
H
Heck, aza-Michael
R2
N
R1
ref 7f,g
R1
N
NH2
Scheme 1. One-pot sulfonylation and intramolecular thia-Michael protocol
As a precursor of the bis-electrophilic synthon vinylsulfonyl chloride, 2-chloroethanesulfonyl chloride (1)
can react with a wide range of bis-nucleophilic synthons, such as substituted 2-amino alcohols, to
generate 7-membered sultams.8 Previous oxa-Michael studies from our laboratories has shown that the
use of a tertiary sulfonamide is necessary for the Michael addition to take place. However, when 1 was
treated with cysteine ethyl ester (2a) or cysteamine (2b), intramolecular thia-Michael and sulfonylation
occur simultaneously, leading to a one-pot synthesis of 1,5,2-dithiazepine 1,1-dioxide scaffolds 3 that

bear free sulfonamide N-H groups (Scheme 1). A survey of base, additive, solvent and temperature
(Entries 1 to 8, Table 1) showed 3.5 equivalent of Et3N, 0.1 equivalent of DMAP, 40 ºC in CH2Cl2 gives
the highest yield. Although the yield in this protocol is only moderate, the reaction is carried out under
mild conditions and the products were conveniently recrystallized and isolated as colorless needles. We
assume the moderate yield may be caused by the polymerization via intermolecular pathways,10 as some
residue that didn’t dissolve in any solvent was observed during recrystallization, as well as the fact that
slightly enhanced yield in more diluted concentration (Entry 9). Another possible reason accounting for
the difficulty in approving the yield could be the decomposition of cysteine ethyl ester. It was supported
by the detection of hydrogen sulfide smell, which is void in either starting cysteine ethyl ester or the
cyclized product. To check this possibility, the ratio of sulfonyl chloride and cysteine ethyl ester was
changed. In the case of excess sulfonyl chloride (Entry 10), the yield was similar to the original result
(Entry 3), while when cysteine ethyl ester was in excess the yield was increased to 42% (Entry 11).
Further increasing cysteine ethyl ester to 4 equivalents resulted in an inseparable byproduct along with the
product (not shown). Finally, the reaction was carried out up to 40-gram scale (cysteine ethyl ester)
using the condition in Entry 3, and roughly 20 grams of the desired product was obtained after
recrystallization (CHCl3) in a single reaction step (Entry 12). The structure of 3b was confirmed via
X-ray crystallography (Figure 1).
Table 1. The synthesis of core scaffolds 3
O
O
S
O
O
HS
NH2
Et3N, DMAP
NH
S
Cl
Cl
CO2Et
CH2Cl2, 40 ºC
CO2Et
S
2b
3b
1
1
2
Base
(equiv.)
En3N (3.5)
En3N (3.5)
En3N (3.5)
Na2CO3
Additive
(equiv.)
DMAP (0.1)
DMAP (0.1)
DMAP (0.1)
-
Solvent
(conc.)
Temp.
(ºC) a
40
40
40
Yield
(%) b
0
30
31
25
0
15
24
Entry
(equiv.) (equiv.)
1
2
3
4
5
6
7
8
9
1
1
1
1
1
1
1
1
1
2
1
1
1.1
1.1
1.1
1.1
1.1
1.1
1.1
1.1
1.1
1
MeOH (0.2 M)
DMF (0.2 M)
CH2Cl2 (0.2 M)
DCM/H2O (0.2 M)
Pyridine (0.2 M)
CH2Cl2 (0.2 M)
DMF (0.2 M)
CH2Cl2 (0.2 M)
CH2Cl2 (0.05 M)
CH2Cl2 (0.2 M)
CH2Cl2 (0.2 M)
CH2Cl2 (0.2 M)
40
40
rt
-
-
En3N (3.5)
En3N (3.5)
En3N (3.5)
En3N (3.5)
En3N (5)
En3N (5)
En3N (3.5)
DMAP (0.1)
DMAP (0.1)
DBU (0.1)
DMAP (0.1)
DMAP (0.1)
DMAP (0.1)
DMAP (0.1)
120 ºC c
40
19
35
26
40
40
40
40
10
11
12
2
1.1
42
41 d
a Reaction time is 14 h.
b Isolated yield after flash chromatography.
c Microwave for 10 minutes.
d Isolated yield for a 40 gram-scale reaction. The product was purified via recrystallization.

O
O
S
NH
CO2Et
S
Figure 1. ORTEP diagram of 3b
Notably, the only report of synthesis of 1,5,2-dithiazepane 1,1-dioxide (3a) involves the usage of
2,2'-dithiobis(ethylamine) dihydrochloride in a two-step sequence using two chromatographic separations
(Scheme 2).11
O
O
O
O
O
O
S
H
N
S
NaBH4
Cl
Cl
NH
S
NH2
S
S
H2N
S
N
S
S
H
O
O
S
1,5,2-dithiazepane 1,1-dioxide (3a)
Scheme 2. Reported synthesis of 1,5,2-dithiazepane 1,1-dioxide (3a)
With the convenient synthesis of sultam 3 in hand, various diversification pathways were explored as
outlined in Scheme 3. Benzylation of sultam scaffolds 3 using substituted benzyl bromides gave
products 4 in good yield. In addition, benzylation of 3a required Cs2CO3 in DMF, but milder conditions
(K2CO3 in MeCN) were sufficient for substrate 3b presumably due to the electron withdrawing ester
group in 3b. Treating of 3 with acyl chloride, in the presence of Et3N and DMAP resulted in
acyl-products 5. Alternatively, reduction of the ester group of 3b followed by isocyanate coupling with
the resulting alcohol provided carbamate 6. Finally, amides 7 were synthesized via a two-step sequence
of benzylation and direct amidation of the ester.

Br
R1
R2
1)
R1
O
O
R2Br, Cs2CO3, DMF, 60 ºC
S
NH
K2CO3, CH3CN, 60 ºC
O
O
O
O
R1
or R2Br, K2CO3, CH3CN, 60 ºC
2) R2NH2, 2-OH Pyr, toluene, 110 ºC
S
S
HN R2
N
N
S
R1
3a R1 = H
3b R1 = CO2Et
O
S
S
O
4a R1 = H, R2 = 4-Br, 68%
1) NaBH4
7a R1 = H, R2 = n-C8H17, 79%
7b R1 = H, R2 = n-C6H13, 99%
7c R1 = H, R2 = i-C4H9, 66%
7d R1 = 4-OMe, R2 = n-C8H17, 59%
7e R1 = 4-OMe, R2 = n-C6H13, 50%
7f R1 = 4-OMe, R2 = i-C4H9, 45%
4b R1 = H, R2 = 3-Cl, 89%
4c R1 = H, R2 = 3-Me, 85%
4d R1 = CO2Me, R2 = H, 71%
4e R1 = CO2Me, R2 = 4-Me, 74%
4f R1 = CO2Me, R2 = 2-F, 81%
Cl
R2
Et3N, DMAP
EtOH-H2O reflux, 79%
2) RNCO
DMAP, CH2Cl2, rt
CH2Cl2, 0 ºC
R
O
O
O
O
O
NH
O
R2
5a R1 = H, R2 = Me, 88%
5b R1 = H, R2 = t-Bu, 87%
S
S
NH
O
N
R1
5c R1 = H, R2 = CH=CHPh, 85%
5d R1 = CO2Me, R2 = t-Bu, 81%
5e R1 = CO2Me, R2 = 3-OMePh, 76%
5f R1 = CO2Me, R2 = CH=CHPh, 79%
S
S
6a R = 4-Me-Ph, 93%
6b R = 4-F-Ph, 66%
Scheme 3. Diversification leading to 1,5,2-dithiazepane 1,1-dioxides
In summary, a one-pot sulfonylation/intramolecular thia-Michael protocol was developed for the
synthesis of 1,5,2-dithiazepine 1,1-dioxide scaffold on multi-gram scale, incorporating stereochemistry
and additional handles for further diversification. A demonstrative library was generated from the
scaffolds. The compounds produced are currently being broadly screened within the NIH Molecular
Library Screening Network (NIH-MLSCN) and additional biological collaborators.
EXPERIMENTAL
All reactions were carried out under argon atmosphere. Stirring was achieved with oven-dried magnetic
stir bars. Et2O, toluene, THF and CH2Cl2 were either purchased through Sigma-Aldrich or purified by
passage through the Solv-Tek purification system employing activated Al2O3 (R. H. Grubbs, R. K. Rosen,
and F. J. Timmers, Organometallics, 1996, 15, 1518–1520). Et3N was purified by passage over basic
alumina or distilled over CaH and stored over KOH. Flash column chromatography was performed with
Sorbent Technologies (30930M-25, Silica Gel 60A, 40-63 um). Thin layer chromatography was
performed on silica gel 60F254 plates (EM-5717, Merck). Deuterated solvents were purchased from
Cambridge Isotope laboratories. 1H, 13C NMR spectra were recorded on a Bruker DRX-400
spectrometer operating at 400 MHz, 100 MHz respectively as well as a Bruker DRX-500 spectrometer
operating at 500 MHz, 125 MHz respectively and a Avance AV-III 500 with a dual carbon/proton
(CPDUL) cryoprobe operating at 500 MHz, 125 MHz respectively. Observed rotations at 589 nm were
measured using AUTOPOL IV Model automatic polarimeter. Weights were taken on a Flexiweigh
Automatic Weigher; weight tolerance +/- 0.3mg. Samples were concentrated on a GeneVac EZ personal
evaporator and placed under high vacuum for ! 2 hours before final weights were taken.

General procedure for the one-pot sulfonylation/intramolecular thia-Michael protocol.
2-Chloroethanesulfonyl chloride (20.9 mL, 0.2 mol) was added drop wise to a stirred solution of cysteine
ethyl ester (40.9 g, 1.1 equiv.) or cysteamine hydrochloride (25.0 g, 1.1 equiv.), Et3N (111.3 mL, 4
equiv.) and DMAP (2.44 g, 0.1 equiv.) in CH2Cl2 (500 mL) at 0 ºC. After addition, the reaction was
allowed to warm to room temperature and stirred overnight. The mixture was washed with 1 M HCl,
brine, dried (Na2CO3), and concentrated under reduced pressure. The crude mixture was recrystallized
with CHCl3 to afford the product 3a or 3b as colorless needles.
1,5,2-Dithiazepane 1,1-dioxide (3a).12
Colorless needles, yield 32%. 1H NMR (500 MHz, CDCl3) " 4.95–4.77 (brs, 1H), 3.55–3.47 (m, 2H),
3.29 (t, J = 6.3 Hz, 2H), 3.10 (t, J = 6.4 Hz, 2H), 2.95–2.86 (m, 2H). 13C NMR (126 MHz, CDCl3) "
58.9, 41.8, 35.1, 28.1.
(R)-Ethyl 1,5,2-dithiazepane-3-carboxylate 1,1-dioxide (3b).
20
Colorless needles, yield 41%. mp 168–169 °C. [!]D -64.8 (c 0.75, CH2Cl2). FTIR: 2947, 1491,
1339, 1140, 1109, 758 cm-1. 1H NMR (500 MHz, CDCl3) " 5.63 (s, 1H), 4.33–4.24 (m, 3H), 3.72 (ddd,
J = 14.4, 3.7 Hz, 3.7 Hz, 1H), 3.53 (dd, J = 15.8, 5.8 Hz, 1H), 3.44 (dd, J = 15.8, 2.9 Hz, 1H), 3.41–3.34
(m, 1H), 2.92–2.88 (m, 2H), 1.31 (t, J = 7.1 Hz, 3H). 13C NMR (126 MHz, CDCl3) " 169.6, 62.9, 59.3,
54.1, 37.3, 28.1, 14.1. HRMS (ESI) m/z calculated for C7H13NNaO4S2 262.0184 (M+Na)+, found
262.0184.
General procedure for the synthesis of 4a-c from scaffolds 3a.
To a stirred solution of 3a (0.2 mmol, 1 equiv.) and benzyl bromide (0.22 mmol, 1.1 equiv.) in DMF
(1 mL) was added Cs2CO3 (0.3 mmol, 1.5 equiv.). The mixture was stirred at 60 ºC overnight, before
quenching with addition of H2O. The mixture was diluted with Et2O, washed with H2O and brine, dried
(Na2CO3), and concentrated under reduced pressure. The crude product was purified via flash
chromatography.
2-(4-Bromobenzyl)-1,5,2-dithiazepane 1,1-dioxide (4a).
White solid, yield 68%. mp 164–165 °C. FTIR: 2924, 2887, 1331, 1142, 914 cm-1. 1H NMR (400
MHz, CDCl3) " 7.52–7.46 (m, 2H), 7.28 (d, J = 8.4 Hz, 2H), 4.48 (s, 2H), 3.52–3.45 (m, 2H), 3.19 (dd, J
= 9.8, 3.5 Hz, 2H), 3.10 (dd, J = 9.7, 3.6 Hz, 2H), 2.94–2.88 (m, 2H). 13C NMR (126 MHz, CDCl3) "
135.1, 131.8, 129.9, 121.9, 55.9, 54.1, 45.5, 34.4, 27.5. HRMS (ESI) m/z calculated for C11H15BrNO2S2
335.9728 (M+H)+, found 335.9733.

2-(3-Chlorobenzyl)-1,5,2-dithiazepane 1,1-dioxide (4b).
White solid, yield 89%. mp 125–126 °C. FTIR: 2924, 1327, 1140, 1121, 854, 735, 494 cm-1. 1H
NMR (500 MHz, CDCl3) " 7.42–7.39 (m, 1H), 7.30 (t, J = 1.4 Hz, 3H), 4.52 (s, 2H), 3.54–3.47 (m, 2H),
3.22 (t, J = 6.3 Hz, 2H), 3.12 (dd, J = 9.6, 3.5 Hz, 2H), 2.95–2.89 (m, 2H). 13C NMR (126 MHz,
CDCl3) " 138.2, 134.7, 130.0, 128.2, 128.2, 126.3, 56.0, 54.2, 45.6, 34.5, 27.5. HRMS (ESI) m/z
calculated for C11H15NO2S2Cl 292.0233 (M+H)+, found 292.0222.
2-(3-Methylbenzyl)-1,5,2-dithiazepane 1,1-dioxide (4c).
White solid, yield 85%. mp 88–89 °C. FTIR: 2919, 1335, 1146, 1124, 854, 729 cm-1. 1H NMR (500
MHz, CDCl3) " 7.26–7.21 (m, 2H), 7.19 (d, J = 7.6 Hz, 1H), 7.13 (d, J = 7.5 Hz, 1H), 4.51 (s, 2H),
3.54–3.47 (m, 2H), 3.22 (t, J = 6.4 Hz, 2H), 3.11 (dd, J = 9.7, 3.4 Hz, 2H), 2.94–2.89 (m, 2H), 2.37 (s,
3H). 13C NMR (126 MHz, CDCl3) " 138.5, 135.9, 128.9, 128.7, 128.5, 125.3, 56.0, 54.5, 45.2, 34.5,
27.5, 21.4. HRMS (ESI) m/z calculated for C12H17NO2S2Na 294.0598 (M+H)+, found 294.0596.
General procedure for the synthesis of 4d–f from scaffolds 3b.
To a stirred solution of 3b (0.20 mmol, 1 equiv.) and benzyl bromide (0.22 mmol, 1.1 equiv.) in CH3CN
(1 mL) was added K2CO3 (0.30 mmol, 1.5 equiv.). The mixture was stirred at 60 ºC overnight, before
quenching with addition of H2O. The mixture was diluted with Et2O, washed with H2O and brine, dried
(Na2CO3), and concentrated under reduced pressure. The crude product was purified via flash
chromatography.
(R)-Ethyl 2-benzyl-1,5,2-dithiazepane-3-carboxylate 1,1-dioxide (4d).
White solid, yield 71%. [!]D20 -31.7 (c 0.70, CH2Cl2). FTIR: 2982, 2926, 1732, 1339, 1148, 854, 727,
698 cm-1. 1H NMR (500 MHz, CDCl3) " 7.53–7.46 (m, 2H), 7.40–7.30 (m, 3H), 4.78 (d, J = 14.6 Hz,
1H), 4.49 (d, J = 14.6 Hz, 1H), 4.09–4.02 (m, 2H), 3.72 (dd, J = 11.0, 7.0 Hz, 1H), 3.59 (ddd, J = 14.5,
3.1, 3.1 Hz, 1H), 3.53 (dd, J = 15.6, 11.0 Hz, 1H), 3.43 (dd, J = 15.6, 7.0 Hz, 1H), 3.29 (ddd, J = 14.5,
12.4, 4.2 Hz, 1H), 2.98 (ddd, J = 15.2, 12.4, 2.8 Hz, 1H), 2.90 (ddd, J = 15.3, 3.6, 3.6 Hz, 1H), 1.17 (t, J =
7.1 Hz, 3H). 13C NMR (126 MHz, CDCl3) " 169.7, 135.1, 129.6, 128.5, 128.2, 61.6, 59.6, 56.2, 55.3,
37.5, 28.4, 13.9. HRMS (ESI) m/z calculated for C14H19NO4S2K 368.0393 (M+K)+, found 303.0392.
(R)-Ethyl 2-(4-methylbenzyl)-1,5,2-dithiazepane-3-carboxylate 1,1-dioxide (4e).
20
Yellow solid, yield 74%. mp 85–86 °C. [!]D -19.5 (c 1.00, CH2Cl2). FTIR: 2980, 2924, 1728,
1340, 1146, 854 cm-1. 1H NMR (400 MHz, CDCl3) " 7.37 (d, J = 8.0 Hz, 2H), 7.16 (d, J = 7.8 Hz, 2H),
4.73 (d, J = 14.4 Hz, 1H), 4.47 (d, J = 14.4 Hz, 1H), 4.06 (q, J = 7.1 Hz, 2H), 3.71 (dd, J = 10.9, 7.1 Hz,
1H), 3.58 (dt, J = 14.5, 3.1 Hz, 1H), 3.52 (dd, J = 15.6, 11.0 Hz, 1H), 3.41 (dd, J = 15.6, 7.1 Hz, 1H),

3.28 (ddd, J = 14.5, 12.2, 4.3 Hz, 1H), 2.97 (ddd, J = 15.0, 12.2, 2.8 Hz, 1H), 2.88 (dt, J = 15.3, 3.9 Hz,
1H), 2.35 (s, 3H), 1.18 (t, J = 7.1 Hz, 3H). 13C NMR (126 MHz, CDCl3) " 169.8, 138.0, 131.9, 129.5,
129.1, 61.6, 59.3, 55.8, 55.3, 37.5, 28.3, 21.1, 13.9. HRMS (ESI) m/z calculated for C15H21NO4S2Na
366.0810 (M+Na)+, found 366.0813.
(R)-Ethyl 2-(2-fluorobenzyl)-1,5,2-dithiazepane-3-carboxylate 1,1-dioxide (4f).
Yellow oil, yield 81%. [!]D20 -6.4 (c 1.00, CH2Cl2). FTIR: 2982, 2926, 1732, 1493, 1342, 1229, 1148,
854, 760 cm-1. 1H NMR (500 MHz, CDCl3) " 7.60 (td, J = 7.6, 1.7 Hz, 1H), 7.35–7.29 (m, 1H), 7.16 (td,
J = 7.5, 1.1 Hz, 1H), 7.11–7.04 (m, 1H), 4.85 (d, J = 15.1 Hz, 1H), 4.55 (d, J = 15.2 Hz, 1H), 4.10 (qd, J
= 7.1, 2.3 Hz, 2H), 3.81 (dd, J = 10.6, 7.4 Hz, 1H), 3.54 (ddd, J = 14.4, 3.0, 3.0 Hz, 1H), 3.50–3.36 (m,
2H), 3.20 (ddd, J = 14.4, 12.5, 4.1 Hz, 1H), 2.96–2.88 (m, 1H), 2.82 (ddd, J = 15.3, 3.7, 3.7 Hz, 1H), 1.18
1
3
(t, J = 7.1 Hz, 3H). 13C NMR (126 MHz, CDCl3) " 169.8, 161.2 (d, JCF = 246.7 Hz), 132.3 (d, JCF
=
3.8 Hz), 130.1 (d, 3JCF = 8.3 Hz), 124.2 (d, 4JCF = 3.6 Hz), 122.5 (d, 2JCF = 14.6 Hz), 115.4 (d, 2JCF = 21.9
5
3
Hz), 61.6, 60.1 (d, JCF = 1.2 Hz), 55.6, 49.1 (d, JCF = 2.9 Hz), 37.1, 28.2, 13.9. HRMS (ESI) m/z
calculated for C14H18FNO4S2Na 370.0559 (M+Na)+, found 370.0555.
General procedure for the synthesis of 5 from scaffolds 3.
To a stirred solution of 3 (0.20 mmol, 1 equiv.), Et3N (0.60 mmol, 3 equiv.), DMAP (0.02 mmol, 0.1
equiv.) in CH2Cl2 (1 mL) was slowly added acyl chloride (0.22 mmol, 1.1 equiv.) at 0 ºC. The mixture
was kept stirring at 0 ºC for 1 h, before quenching with addition of H2O. The mixture was diluted with
Et2O, washed with H2O and brine, dried (Na2CO3), and concentrated under reduced pressure. The crude
product was purified via flash chromatography.
1-(1,1-Dioxido-1,5,2-dithiazepan-2-yl)ethanone (5a).
Yellow solid, yield 88%. mp 104–105 °C. FTIR: 2984, 2926, 1697, 1352, 1157, 849 cm-1. 1H NMR
(400 MHz, CDCl3) " 3.99 (d, J = 6.7 Hz, 2H), 3.68–3.62 (m, 2H), 3.10 (d, J = 6.6 Hz, 2H), 2.92–2.87 (m,
2H), 2.46 (s, 3H). 13C NMR (126 MHz, CDCl3) " 171.0, 55.4, 42.9, 33.5, 27.4, 25.1. HRMS (ESI) m/z
calculated for C6H11NO3S2Na 232.0078 (M+Na)+, found 232.0078.
1-(1,1-Dioxido-1,5,2-dithiazepan-2-yl)-2,2-dimethylpropan-1-one (5b).
1
White solid, yield 87%. mp 125–126 °C. FTIR: 2976, 2935, 1688, 1352, 1157, 1095 cm-1. H NMR
(400 MHz, CDCl3) " 3.93 (t, J = 6.5 Hz, 2H), 3.90–2.83 (m, 2H), 3.16 (t, J = 6.5 Hz, 2H), 2.91–2.86 (m,
2H), 1.35 (s, 9H). 13C NMR (126 MHz, CDCl3) " 181.2, 54.2, 44.5, 41.7, 35.9, 28.8, 28.0. HRMS
(ESI) m/z calculated for C9H17NO3S2Na 274.0548 (M+Na)+, found 274.0530.

(E)-1-(1,1-Dioxido-1,5,2-dithiazepan-2-yl)-3-phenylprop-2-en-1-one (5c).
1
White solid, yield 85%. mp 150–151 °C. FTIR: 2926, 1676, 1616, 1356, 1155, 1122 cm-1. H NMR
(400 MHz, CDCl3) " 7.84 (d, J = 15.4 Hz, 1H), 7.60 (dd, J = 6.8, 2.8 Hz, 2H), 7.48–7.41 (m, 2H), 4.15 (t,
J = 6.6 Hz, 2H), 3.71–3.65 (m, 2H), 3.15 (t, J = 6.6 Hz, 2H), 2.93–2.87 (m, 2H). 13C NMR (126 MHz,
CDCl3) " 166.7, 147.3, 134.3, 130.8, 129.0, 128.5, 117.3, 56.8, 42.6, 33.9, 27.7. HRMS (ESI) m/z
calculated for C13H15NO3S2Na 320.0391 (M+Na)+, found 320.0393.
(R)-Ethyl 2-pivaloyl-1,5,2-dithiazepane-3-carboxylate 1,1-dioxide (5d).
20
White solid, yield 81%. mp 152–153 °C. [!]D -102.2 (c 0.92, CH2Cl2). FTIR: 2954, 1738, 1690,
1
1358, 1159, 1111 cm-1. H NMR (500 MHz, CDCl3) " 4.85 (dd, J = 9.2, 8.1 Hz, 1H), 4.29 (qd, J = 7.1,
1.6 Hz, 2H), 4.21 (ddd, J = 14.8, 9.2, 7.9 Hz, 1H), 3.60 (dd, J = 15.7, 9.4 Hz, 1H), 3.55–3.47 (m, 2H),
2.91 (dd, J = 2.9, 1.5 Hz, 1H), 2.90 (d, J = 3.0 Hz, 1H), 1.37 (s, 9H), 1.32 (t, J = 7.1 Hz, 3H). 13C NMR
(126 MHz, CDCl3) " 179.8, 168.9, 62.3, 57.4, 53.3, 41.6, 38.4, 28.8, 28.2, 14.0. HRMS (ESI) m/z
calculated for C12H21NO5S2Na 346.0759 (M+Na)+, found 346.0741.
(R)-Ethyl 2-(3-methoxybenzoyl)-1,5,2-dithiazepane-3-carboxylate 1,1-dioxide (5e).
20
White solid, yield 76%. mp 112–113 °C. [!]D -83.2 (c 1.00, CH2Cl2). FTIR: 2981, 1740, 1690,
1364, 1231, 1161, 841, 746 cm-1. 1H NMR (500 MHz, CDCl3) " 7.37 (t, J = 7.9 Hz, 1H), 7.29 (dd, J =
7.6, 1.0 Hz, 1H), 7.27–7.25 (m, 1H), 7.07 (ddd, J = 8.3, 2.6, 0.9 Hz, 1H), 4.60 (dd, J = 10.0, 7.7 Hz, 1H),
4.41–4.30 (m, 3H), 3.83 (s, 3H), 3.64 (dt, J = 14.9, 3.0 Hz, 1H), 3.50 (dd, J = 15.6, 10.0 Hz, 1H), 3.36 (dd,
J = 15.6, 7.7 Hz, 1H), 3.04–2.91 (m, 2H), 1.37 (t, J = 7.1 Hz, 3H). 13C NMR (126 MHz, CDCl3) " 173.0,
168.6, 159.8, 135.1, 130.2, 119.8, 118.4, 112.5, 62.5, 60.3, 55.4, 53.6, 38.2, 29.0, 14.1. HRMS (ESI)
m/z calculated for C15H19NO6S2Na 396.0551 (M+Na)+, found 396.0567.
(R)-Ethyl 2-cinnamoyl-1,5,2-dithiazepane-3-carboxylate 1,1-dioxide (5f).
20
Yellow solid, yield 79%. mp 123–124 °C. [!]D -83.2 (c 1.00, CH2Cl2). FTIR: 2981, 1740, 1690,
1364, 1231, 1161, 841, 746 cm-1. 1H NMR (400 MHz, CDCl3) " 7.90 (d, J = 15.4 Hz, 1H), 7.64–7.58
(m, 2H), 7.53 (d, J = 15.4 Hz, 1H), 7.43 (dd, J = 5.1, 1.9 Hz, 3H), 5.56 (dd, J = 11.4, 6.4 Hz, 1H), 4.32
(dq, J = 10.7, 7.1 Hz, 1H), 4.23 (dq, J = 10.7, 7.1 Hz, 1H), 3.72 (dt, J = 14.6, 3.4 Hz, 1H), 3.66–3.56 (m,
1H), 3.52 (d, J = 9.0 Hz, 2H), 3.04–2.88 (m, 2H), 1.31 (t, J = 7.1 Hz, 3H). 13C NMR (126 MHz, CDCl3)
" 169.1, 166.2, 148.1, 134.2, 131.0, 129.0, 128.6, 116.6, 62.1, 56.9, 55.1, 36.1, 28.0, 14.1. HRMS (ESI)
m/z calculated for C16H19NO5S2Na 392.0602 (M+Na)+, found 392.0605.
General procedure for the synthesis of 6 from scaffolds 3b.

A solution of NaBH4 (24 mmol, 4 equiv.) in 50% ethanol/H2O (10 mL) was added to a suspension of 3b
(6 mmol, 1 equiv.) in 50% EtOH/H2O (10 mL). After the reaction was stirred at 80 ºC for 4 h, solvent
was evaporated to afford the crude alcohol, which was purified via flash chromatography. The resulting
alcohol (0.2 mmol, 1 equiv.), DMAP (0.02 mmol, 0.1 equiv.) was dissolved in CH2Cl2 (1 mL), then
isocyanate (0.21 mmol, 1.05 equiv.) was added at rt. The reaction was stirred at rt for 2 h and the
solvent was removed under reduced pressure. The resulted residue was dissolved back into EtOAc,
washed with 10% HCl and brine, and dried (Na2SO4). The crude product was purified via flash
chromatography.
(R)-(1,1-Dioxido-1,5,2-dithiazepan-3-yl)methyl p-tolylcarbamate (6a).
20
White solid, yield 93%. mp 192–193 °C. [!]D 16.3 (c 0.40, MeOH). FTIR: 3304, 2918, 1728,
1531, 1315, 1294, 1223, 1207, 1128, 1063, 816 cm-1. 1H NMR (500 MHz, DMSO) " 9.62 (s, 1H), 7.85 (d,
J = 6.4 Hz, 1H), 7.34 (d, J = 7.9 Hz, 2H), 7.08 (d, J = 8.3 Hz, 2H), 4.03 (ddd, J = 17.3, 10.9, 7.0 Hz, 2H),
3.56–3.35 (m, 3H), 3.10 (dd, J = 15.1, 4.9 Hz, 1H), 2.91 (dd, J = 15.1, 8.7 Hz, 1H), 2.86 (dd, J = 8.2, 3.8
Hz, 2H), 2.23 (s, 3H). 13C NMR (126 MHz, DMSO) " 153.1, 136.4, 131.3, 129.1, 118.2, 65.6, 59.1,
52.4, 35.9, 27.3, 20.3. HRMS (ESI) m/z calculated for C13H18N2O4S2Na 353.0606 (M+Na)+, found
353.0611.
(R)-(1,1-Dioxido-1,5,2-dithiazepan-3-yl)methyl (4-fluorophenyl)carbamate (6b).
20
White solid, yield 66%. mp 163–164 °C. [!]D 16.0 (c 0.40, MeOH). FTIR: 3308, 2918, 1717,
1508, 1325, 1213, 1128, 1063, 816 cm-1. 1H NMR (500 MHz, Acetone) " 8.81 (s, 1H), 7.57 (dd, J = 8.8,
4.8 Hz, 2H), 7.12–7.03 (m, 2H), 6.79 (d, J = 5.4 Hz, 1H), 4.18 (qd, J = 10.9, 6.9 Hz, 2H), 3.66–3.58 (m,
1H), 3.56–3.49 (m, 1H), 3.44 (ddd, J = 14.4, 7.2, 5.3 Hz, 1H), 3.21 (dd, J = 15.3, 5.2 Hz, 1H), 3.04 (dd, J
1
= 15.3, 8.6 Hz, 1H), 2.96–2.91 (m, 2H). 13C NMR (126 MHz, Acetone) " 159.2 (d, JCF = 239.5 Hz),
154.0, 136.2, 120.7, 115.9 (d, 2JCF = 22.5 Hz), 79.0, 66.9, 59.9, 36.7, 28.5. HRMS (ESI) m/z calculated
for C12H15FN2O4S2Na 357.0355 (M+Na)+, found 357.0353.
General procedure for the synthesis of 7 from scaffolds 4.
A solution of scaffold 4 (0.2 mmol, 1 equiv.), amine (2 mmol, 10 equiv.) and 2-hydroxypyridine (0.04
mmol, 0.2 equiv.) in toluene (1 mL) was refluxed for 14 h. The solvent was evaporated under reduced
pressure and the crude product was purified via flash chromatography.
(R)-2-Benzyl-N-octyl-1,5,2-dithiazepane-3-carboxamide 1,1-dioxide (7a).
20
White solid, yield 79%. mp 136–137 °C. [!]D -0.8 (c 1.00, CH2Cl2). FTIR: 3429, 2922, 2851,

1668, 1327, 1140, 1051, 854, 733, 706 cm-1. 1H NMR (500 MHz, CDCl3) " 7.48–7.43 (m, 2H), 7.41–7.32
(m, 3H), 6.33 (s, 1H), 4.86 (d, J = 14.1 Hz, 1H), 4.22 (d, J = 14.1 Hz, 1H), 3.77–3.70 (m, 2H), 3.57 (dd, J
= 15.8, 5.8 Hz, 1H), 3.47–3.35 (m, 2H), 3.01 –2.93 (m, 3H), 2.88 (dt, J = 15.3, 3.5 Hz, 1H), 1.36–1.09 (m,
12H), 0.90 (t, J = 7.1 Hz, 3H). 13C NMR (126 MHz, CDCl3) " 169.0, 135.1, 129.3, 129.1, 128.8, 64.6,
57.5, 56.1, 39.5, 37.7, 31.8, 29.1, 29.1, 29.1, 27.8, 26.6, 22.6, 14.1. HRMS (ESI) m/z calculated for
C20H32N2O3S2Na 435.1752 (M+Na)+, found 435.1756.
(R)-2-Benzyl-N-hexyl-1,5,2-dithiazepane-3-carboxamide 1,1-dioxide (7b).
20
White solid, yield 99%. mp 138–140 °C. [!]D -1.6 (c 0.75, CH2Cl2). FTIR: 3431, 2951, 2926,
1
2856, 1668, 1327, 1140, 1051, 854, 733, 706 cm-1. H NMR (500 MHz, CDCl3) " 7.45 (dd, J = 7.9, 1.4
Hz, 2H), 7.42–7.35 (m, 3H), 6.33 (s, 1H), 4.86 (d, J = 14.1 Hz, 1H), 4.22 (d, J = 14.1 Hz, 1H), 3.73 (ddd,
J = 14.6, 8.9, 4.4 Hz, 2H), 3.57 (dd, J = 15.8, 5.8 Hz, 1H), 3.47–3.35 (m, 2H), 3.04–2.93 (m, 3H), 2.88 (dt,
J = 15.4, 3.4 Hz, 1H), 1.34–1.11 (m, 8H), 0.89 (t, J = 7.2 Hz, 3H). 13C NMR (126 MHz, CDCl3) " 169.0,
135.1, 129.3, 129.1, 128.8, 64.6, 57.5, 56.1, 39.4, 37.7, 31.4, 29.0, 27.8, 26.3, 22.5, 14.0. HRMS (ESI)
m/z calculated for C18H28N2O3S2Na 407.1439 (M+Na)+, found 407.1440.
(R)-2-Benzyl-N-isobutyl-1,5,2-dithiazepane-3-carboxamide 1,1-dioxide (7c).
20
White solid, yield 66%. mp 196–197 °C. [!]D -0.9 (c 1.00, CH2Cl2). FTIR: 3427, 2957, 2918,
2870, 1668, 1522, 1329, 1138, 1049, 854, 735 cm-1. 1H NMR (500 MHz, CDCl3) " 7.48–7.43 (m, 2H),
7.42–7.33 (m, 3H), 6.42 (s, 1H), 4.85 (d, J = 14.1 Hz, 1H), 4.24 (d, J = 14.1 Hz, 1H), 3.80–3.69 (m, 2H),
3.58 (dd, J = 15.7, 5.8 Hz, 1H), 3.47–3.36 (m, 2H), 3.00 (ddd, J = 15.3, 12.6, 2.7 Hz, 1H), 2.92–2.85 (m,
2H), 2.77–2.69 (m, 1H), 1.53 (td, J = 13.4, 6.7 Hz, 1H), 0.79 (d, J = 6.7 Hz, 2H), 0.76 (d, J = 6.7 Hz, 2H).
13C NMR (126 MHz, CDCl3) " 169.2, 135.1, 129.3, 129.2, 128.8, 64.5, 57.4, 56.0, 46.7, 37.8, 28.2, 27.9,
19.9, 19.9. HRMS (ESI) m/z calculated for C16H24N2O3S2Na 379.1126 (M+Na)+, found 379.1128.
(R)-2-(4-Methoxybenzyl)-N-octyl-1,5,2-dithiazepane-3-carboxamide 1,1-dioxide (7d).
20
White solid, yield 59%. mp 159–160 °C. [!]D -0.8 (c 0.75, CH2Cl2). FTIR: 3431, 2953, 2922,
1
2851, 1664, 1512, 1327, 1151, 1140, 1057, 847 cm-1. H NMR (500 MHz, CDCl3) " 7.39–7.34 (m, 2H),
6.92–6.86 (m, 2H), 6.32 (s, 1H), 4.80 (d, J = 14.1 Hz, 1H), 4.17 (d, J = 14.0 Hz, 1H), 3.81 (s, 3H),
3.78–3.68 (m, 2H), 3.56 (dd, J = 15.7, 5.8 Hz, 1H), 3.45–3.32 (m, 2H), 2.99 (tdd, J = 12.1, 7.4, 4.7 Hz,
3H), 2.86 (dt, J = 15.3, 3.5 Hz, 1H), 1.34–1.10 (m, 12H), 0.89 (t, J = 7.1 Hz, 3H). 13C NMR (126 MHz,
CDCl3) " 169.1, 159.9, 130.6, 127.0, 114.4, 64.4, 56.9, 56.1, 55.2, 39.5, 37.6, 31.8, 29.2, 29.1, 27.8, 26.7,
22.6, 14.1. HRMS (ESI) m/z calculated for C21H35N2O4S2 443.2038 (M+H)+, found 443.2046.

(R)-N-Hexyl-2-(4-methoxybenzyl)-1,5,2-dithiazepane-3-carboxamide 1,1-dioxide (7e).
20
White solid, yield 50%. mp 165–166 °C. [!]D -1.2 (c 0.85, CH2Cl2). FTIR: 3431, 2955, 2924,
2854, 1664, 1512, 1327, 1151, 1140, 1057, 849 cm-1. 1H NMR (500 MHz, CDCl3) " 7.38–7.34 (m, 2H),
6.92–6.87 (m, 2H), 6.32 (s, 1H), 4.80 (d, J = 14.0 Hz, 1H), 4.16 (d, J = 14.0 Hz, 1H), 3.81 (s, 3H),
3.77–3.67 (m, 2H), 3.57 (dd, J = 15.7, 5.8 Hz, 1H), 3.48–3.32 (m, 2H), 3.07–2.92 (m, 3H), 2.86 (dt, J =
15.4, 3.5 Hz, 1H), 1.36–1.10 (m, 8H), 0.88 (t, J = 7.1 Hz, 3H). 13C NMR (126 MHz, CDCl3) " 169.1,
159.9, 130.6, 127.0, 114.4, 64.4, 56.9, 56.1, 55.2, 39.4, 37.6, 31.4, 29.1, 27.8, 26.3, 22.5, 14.0. HRMS
(ESI) m/z calculated for C19H30N2O4S2Na 437.1545 (M+Na)+, found 437.1552.
(R)-N-Isobutyl-2-(4-methoxybenzyl)-1,5,2-dithiazepane-3-carboxamide 1,1-dioxide (7f).
20
White solid, yield 45%. mp 175–176 °C. [!]D -0.7 (c 0.90, CH2Cl2). FTIR: 3427, 2955, 2926,
1
1664, 1514, 1327, 1153, 1140, 1126, 1057, 855, 739 cm-1. H NMR (500 MHz, CDCl3) " 7.39–7.33 (m,
2H), 6.94–6.85 (m, 2H), 6.42 (s, 1H), 4.79 (d, J = 14.0 Hz, 1H), 4.19 (d, J = 14.0 Hz, 1H), 3.81 (s, 3H),
3.76 (dd, J = 11.9, 5.9 Hz, 1H), 3.70 (dt, J = 14.7, 3.0 Hz, 1H), 3.57 (dd, J = 15.7, 5.8 Hz, 1H), 3.43 (dd, J
= 15.7, 12.0 Hz, 1H), 3.37 (ddd, J = 14.6, 12.7, 3.9 Hz, 1H), 2.99 (ddd, J = 15.3, 12.7, 2.7 Hz, 1H),
2.93–2.84 (m, 2H), 2.81–2.74 (m, 1H), 1.57–1.51 (m, 1H), 0.79 (d, J = 6.7 Hz, 3H), 0.76 (d, J = 6.7 Hz,
3H). 13C NMR (126 MHz, CDCl3) " 169.3, 159.9, 130.6, 127.0, 114.5, 64.3, 56.9, 56.0, 55.3, 46.8, 37.8,
28.2, 27.9, 19.9, 19.8. HRMS (ESI) m/z calculated for C17H26N2O4S2Na 409.1232 (M+Na)+, found
409.1221.
ACKNOWLEDGEMENTS
This work was generously supported by funds provided by the Institute of General Medical Sciences
(P50-GM069663 and P41-GM076302), NIH K-INBRE (D.B., P20 RR016475), and the University of
Kansas for an Undergraduate Research Award (D.B.). All are gratefully acknowledged.
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12. A two-step route for the synthesis of compound 3a has been previously reported, see ref 11.