An Efficient Synthesis of Sialoglycoconjugates on a Peptidase-Sensitive Polymer Support

Article abstract of DOI:10.1016/0040-4039(95)02056-X

A novel method for the enzymatic synthesis of oligosaccharide derivatives on a α-chymotrypsin-sensitive polymer support is described.The primer polymer having N-acetyl-D-glucosamine (GlcNAc) residue through a phenylalanine-containing spacer moiety was successfully elongated with galactosyl and sialyltransferases to give a glycopolymer bearing sialyl α(2->) N-acetyllactosamine branches in high yield.Subsequent hydrolysis with α-chymotripsin proceeded smoothly and afforded a versatile sialotrisaccharide derivative having a terminal amino group which can be used for creating neoglycoconjugates.