Tetrahedron p. 11317 - 11346 (1997)
Update date:2022-07-29
Topics: Solid-phase synthesis Chemical modification Purification Fluorescent Labeling Hybridization Nucleotide Oligonucleotide
Grotli, Morten
Eritja, Ramon
Sproat, Brian
An effective method for synthesising branched oligonucleotides on solid phase in the 5' to 3' direction has been developed. Special branch-point monomers enable the synthesis of branched oligonucleotides which can have sequences of different length and base composition attached to the 2'- and 3'-hydroxyl groups of the branch point ribonucleoside. The branched oligonucleotides are assembled on commercial DNA synthesisers, the crude products are readily purified by reversed phase HPLC and the fully deprotected products are conveniently analysed by mass spectrometry.
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(2019)
