593-84-0 Usage
Uses
Used in Molecular Biology:
Guanidine thiocyanate is used as a potent protein denaturant for the isolation of intact ribonucleic acid (RNA) and deoxyribonucleic acid (DNA) to eliminate RNase activity. It is used to inactivate RNase when isolating RNA from tissues that are rich in RNase, such as liver. Total nuclear and cytoplasmic RNA may be isolated this way.
Used in Virology:
Guanidine thiocyanate is utilized to deactivate the influenza virus, making it a useful tool in virology research and applications.
Used in Diagnostics:
It acts as a medium for blood sample lysates in order to maintain the integrity of nucleic acids, which is important for accurate diagnostics and analysis.
Used in Quantification of mRNA:
Guanidine thiocyanate is involved in the quantification of messenger RNA (mRNA) from hepatocytes, aiding in research and understanding of gene expression.
Used in Stabilization Buffers:
Guanidine thiocyanate has been used as a component of the stabilization buffer for RNA lysis, helping to denature nucleoprotein complexes and deactivate endogenous RNases.
Used in Denaturation of Macromolecules:
It is suitable for denaturation of macromolecules such as nucleic acids and proteins, which is essential in various molecular biology techniques and applications.
Biochem/physiol Actions
Guanidine thiocyanate is a denaturing agent and is used routinely in RNA isolation. It is used as a storage buffer for whole blood samples. Guanidine thiocyanate inactivates nucleases and is ideal for storing and freezing fecal samples for DNA studies. It is used in combination with phenol?chloroform in RNA extraction.
Biochem/physiol Actions
Guanidine thiocyanate (GTC) plays a role in protein denaturation. It is involved in separation of ribosomal ribonucleic acid (rRNA) from ribosomes and denatures ribonuclease (RNase). GTC has been studied in the unfolding and refolding of fluorescent proteins (FP). It can be used along with phenol and chloroform for extraction of RNA.
Toxicity evaluation
Guanidine thiocyanate is used commercially as a disinfectant and a general protein denaturant but its more common use is in the extraction of DNA and RNA in molecular biology. Нere are no reports of guanidine thiocyanate toxicity from oral ingestion. Toxicity of di??erent thiocyanate compounds other than guanidine thiocyanate has been described. Potassium thiocyanate was used over 50 years ago for the treatment of hypertension and its toxicity appears to cause a neurological disorder consisting of generalized weakness, delirium and a decrease level of consciousness. Reports of thiocyanate toxicity from nitroprusside use for hypertension in the setting of renal impairment/failure have also been reported as thiocyanate is renally cleared. In a fatal deliberate ingestion of a herbicide which contained ammonium thiocyanate and aminotriazole, a 54 year old man presented with coma and cardiovascular collapse although the relative contributions of the two herbicides to the clinical toxicity is unknown.
Hazard
Irritant.
Flammability and Explosibility
Notclassified
Check Digit Verification of cas no
The CAS Registry Mumber 593-84-0 includes 6 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 3 digits, 5,9 and 3 respectively; the second part has 2 digits, 8 and 4 respectively.
Calculate Digit Verification of CAS Registry Number 593-84:
(5*5)+(4*9)+(3*3)+(2*8)+(1*4)=90
90 % 10 = 0
So 593-84-0 is a valid CAS Registry Number.
InChI:InChI=1/C2H4N4S/c3-1-7-6-2(4)5/h(H4,4,5,6)
593-84-0Relevant articles and documents
Kinetics of thermal decomposition of thiourea
Timchenko,Novozhilov,Slepysheva
, p. 1046 - 1050 (2007/10/03)
Thermal decomposition of thiourea in a melt was studied by isothermal and nonisothermal kinetic methods. The influence of the gas flow rate over the melt, layer thickness, and other factors on the process was elucidated. The kinetic parameters of the steps of formation of guanidinium thiocyanate and solid cyclic compounds, products of thiourea thermolysis, were determined.
