Highly efficient synthesis of 2'-O-amino nucleosides and their incorporation in hammerhead ribozymes

Article abstract of DOI:10.1016/S0040-4039(97)10784-5

2'-O-Amino nucleosides were prepared from arabino nucleosides by triflate displacement with N-hydroxy phthalimide. The corresponding phosphoramidites suitable for automated oligonucleotide synthesis, were also prepared. Incorporation of 2'-O-amino uridine into position U7 of 2'-O-amino adenosine into position A9 of a hammerhead ribozyme resulted in a slight improvement of catalytic rates.