Tetrahedron p. 13547 - 13556 (1998)
Update date:2022-07-31
Topics:
Beaussire, Jean-Jacques
Pochet, Sylvie
The hybridization properties of 5-methyl-isocytosine ((5Me)isoC) and isohypoxanthine (isoI) have been investigated by using ultraviolet melting techniques. These two bases were incorporated into heptadecanucleotides using the phosphoramidite chemistry. The base pair (5Me)isoC/isoI was found to be isoenergetic with the A/T base pair. A small destabilization was observed when isoI was located opposite T or G and can be interpreted by a pairing mode between these bases and isol in its lactam form.
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