The β oxidative cleavage of long chain fatty acids in rat liver cytoplasm

Article abstract of DOI:10.1111/j.1432-1033.1973.tb03087.x

A β oxidative cleavage of oleyl and stearyl CoA has been obtained with soluble enzymes of rat liver homogenate. The transformation requires NAD⁺ as an oxido reductive cofactor and is enhanced by ATP and CoA. Under the reported conditions 2,9 octadecadienoic, 3,9 octadecadienoic, 3 hydroxy 9 octadecenoic, 7 hexadecenoic, 3 hydroxy 7 hexadecenoic and 5 tetradecenoic acids are present after incubation of oleyl CoA, while no detectable amounts of shorter fatty acids are formed. A similar oxidation pattern is obtained with stearyl CoA when Mg²⁺ ions are also added to the system. The cytoplasmic origin of these enzymes is established by comparing their activity with that of the mitochondrial β oxidation enzymes.