Introduction of 6-formylcytidine into a MYB binding sequence

Article abstract of DOI:10.1080/07328319908044640

Single 6-formylcytidine was introduced into a oligonucleotide duplex (23 mers) as a substitute for thymidine in the Myb binding sequence of 3'-TTGAC- 5'. The modified duplex showed Tm of 67 °C, which was six degrees lower than the Tm of the native duplex. Binding affinity of the 23-mers to the Myb protein was estimated by electrophoretic mobility shift assays, and the binding was almost completely abolished.

Full text of DOI:10.1080/07328319908044640

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Nucleosides and Nucleotides
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Introduction of 6-Formylcytidine into a
Myb Binding Sequence
Atushi Kittaka a , Tesuya Kuze a , Miori Amano a , Hiomichi Tanaka a ,
Taashi Miyasaka a , Kuihiko Hirose b , Taao Yoshida b , Aknori Sarai c ,
Taashi Yasukawa c & Shnsuke Ishii c
a School of Pharmaceutical Sciences , Showa University , 1-5-8
Hatanodai, Shinagawa-ku, Tokyo, 142-8555, Japan
b Toagosei Co., Ltd. , 2 Ohkubo, Tsukuba, Ibaraki, 300-2611, Japan
c Tsukuba Life Science Center, The Institute of Physical and Chemical
Research , Tsukuba, Ibaraki, 305-0074, Japan
Published online: 04 Oct 2006.
To cite this article: Atushi Kittaka , Tesuya Kuze , Miori Amano , Hiomichi Tanaka , Taashi Miyasaka ,
Kuihiko Hirose , Taao Yoshida , Aknori Sarai , Taashi Yasukawa & Shnsuke Ishii (1999) Introduction of 6-
Formylcytidine into a Myb Binding Sequence, Nucleosides and Nucleotides, 18:11-12, 2769-2783, DOI:
10.1080/07328319908044640
To link to this article: http://dx.doi.org/10.1080/07328319908044640
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