Phytochemical and Analytical Characterization of Novel Sulfated Coumarins in the Marine Green Macroalga Dasycladus vermicularis (Scopoli) Krasser

Article abstract of DOI:10.3390/molecules23112735

The siphonous green algae form a morphologically diverse group of marine macroalgae which include two sister orders (Bryopsidales and Dasycladales) which share a unique feature among other green algae as they are able to form large, differentiated thalli

Full text of DOI:10.3390/molecules23112735

molecules
Article
Phytochemical and Analytical Characterization of
Novel Sulfated Coumarins in the Marine Green
Macroalga Dasycladus vermicularis (Scopoli) Krasser
Anja Hartmann 1,*, Markus Ganzera 1 , Ulf Karsten 2 , Alexsander Skhirtladze 3 and
Hermann Stuppner 1
1
Institute of Pharmacy, Pharmacognosy, CMBI, University of Innsbruck, Innrain 80-82,
6020 Innsbruck, Austria; markus.ganzera@uibk.ac.at (M.G.); hermann.stuppner@uibk.ac.at (H.S.)
Institute of Biological Sciences, Applied Ecology & Phycology, University of Rostock, Albert-Einstein-Str.
2
3, 18059 Rostock, Germany; ulf.karsten@uni-rostock.de
Department of Phytochemistry, Iovel Kutateladze Institute of Pharmacochemistry, Tbilisi State Medical
3
University, 0159 Tbilisi, Georgia; aleksandre.skhirtladze@yahoo.com
*
Correspondence: Anja.Hartmann@uibk.ac.at; Tel.: +43-512-507-58430
ꢀꢁꢂꢀꢃꢄꢅꢆꢇ
Academic Editor: Pinarosa Avato
Received: 11 September 2018; Accepted: 17 October 2018; Published: 23 October 2018
ꢀꢁꢂꢃꢄꢅꢆ
Abstract: The siphonous green algae form a morphologically diverse group of marine macroalgae
which include two sister orders (Bryopsidales and Dasycladales) which share a unique feature
among other green algae as they are able to form large, differentiated thalli comprising of a
single, giant tubular cell. Upon cell damage a cascade of protective mechanisms have evolved
including the extrusion of sulfated metabolites which are involved in the formation of a rapid
wound plug. In this study, we investigated the composition of sulfated metabolites in Dasycladus
vermicularis (Dasycladales) which resulted in the isolation of two phenolic acids and four coumarins
including two novel structures elucidated by nuclear magnetic resonance spectroscopy (NMR) as
5,80-di-(6(60),7(70)-tetrahydroxy-3-sulfoxy-30-sulfoxycoumarin), a novel coumarin called dasycladin
A and 7-hydroxycoumarin-3,6-disulfate, which was named dasycladin B. In addition, an analytical
assay for the chromatographic quantification of those compounds was developed and performed on
a reversed phase C-18 column. Method validation confirmed that the new assay shows good linearity
(R2
≥
0.9986), precision (intra-day R.S.D
≤
3.71%, inter-day R.S.D
≤
7.49%), and accuracy (recovery
rates ranged from 104.06 to 97.45%). The analysis of several samples of Dasycladus vermicularis from
different collection sites, water depths and seasons revealed differences in the coumarin contents,
ranging between 0.26 to 1.61%.
Keywords: siphonous green algae; sulfated coumarins; Dasycladus vermicularis; isolation
and quantification
1. Introduction
Dasycladus vermicularis (Scopoli) Krasser is an evolutionarily ancient, small siphonous green alga,
which is widely distributed throughout tropical to temperate regions such as several Atlantic islands
(Canary islands, Madeira, Antilles), many regions in the Mediterranean Sea, Central America (Belize),
Caribbean islands, South America (Brazil), and Asia (Japan, South China Sea, Philippines) [
chlorophyte inhabits well-illuminated shallow waters (0.3–20 m) with high light exposures in the
upper littoral zone on rocky substrates and are often covered by a thin layer of sediment [ ]. To thrive
under enhanced doses of UV radiation, photo-protective mechanisms are needed. In 1983, Menzel
et al. [ ] isolated and identified 3,6,7-trihydroxycoumarin (thyc) as a UV absorbing compound from
1]. This
2
3
Molecules 2018, 23, 2735; doi:10.3390/molecules23112735
www.mdpi.com/journal/molecules

Molecules 2018, 23, 2735
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D. vermicularis for the first time, which was also the first report of coumarins in algae. Subsequently,
several studies have been carried out to investigate the relevance of thyc in D. vermicularis. They
indicated an elevated excretion of thyc due to increasing UV exposure and temperatures suggesting
that this compound is a natural sunscreen/UV protectant [4–6]. Another very remarkable characteristic
of D. vermicularis is its morphology. D. vermicularis is a member of the so-called siphonous green macro
algae comprising unique giant single cells without cross walls. These thalli can grow up to 10 cm
long, gaining stability by surrounding themselves with a calcareous coating which supports the long
unicellular algae with sufficient stability to grow upright. Siphonous algae typically contain a huge
central vacuole and a thin layer of cytoplasm, the latter inhabiting multiple nuclei (Bryopsidales) or
just one nucleus (Dasycladales) [7]. After cell damage, for example due to herbivory, a rapid wound
closure is essential for the survival of such organisms. Therefore, immediately upon injury a cascade
of biochemical reactions is induced to assimilate cellular contents into an insoluble wound plug
initially formed by gelling, followed by a slower hardening process (1–2 h) [
indispensable to avoid cytoplasmic loss and limits the intrusion of extracellular components, herbivore
attack or pathogenic invasion, which could otherwise result in high mortality rates [ 10]. A few
years ago Welling et al. [ ] investigated both the intact and wounded alga to monitor changes in
8,9]. This mechanism is
8,
8
chemical composition, which may be involved in the wound plug formation. This study surprisingly
revealed the dominant secondary metabolite to be 6,7-dihydroxycoumarin-3-sulfate (dhycs) in
the methanolic extracts of intact D. vermicularis, while the previously reported major compound
3,6,7-trihydroxycoumarin was only found in the methanolic extracts after wounding. Thus, the dhycs is
supposed to act as a precursor and is transformed into the more active thyc in the presence of sulfatases.
According to Welling et al. [9] thyc acts as an intermediate which is rapidly oxidized and serves as a
protein cross-linker for the formation of a wound sealing co-polymer in combination with amino acid
side chains from the alga. Such polymerization processes are known from other marine organisms,
which are usually involved in bioadhesive processes that are needed for sessile marine organisms
such as tubeworms and mussels to attach themselves to surfaces. For example, the common blue
mussel Mytilus edulis uses a metal centered chelate that initiates the biopolymerisation process which
includes secondary metabolites such as protein-bound-dopamine [11]. Sulfated secondary metabolites
are widely distributed among marine species and are stored therein in a dormant state. They are then
transformed enzymatically into more active metabolites, for example, psammaplin A sulfate from the
sponge Aplysinella rhax or zosteric acid, an antifouling metabolite in the seagrass Zostera marina. Both
metabolites are converted upon tissue disruption to their desulfated form thereby increasing activity
as a defensive metabolite [12,13]. Kurth et al. [14] just recently revealed the presence of two sulfated
phenolic acids in D. vermicularis namely 4-(sulfooxy)benzoic acid (SBA) and 4-(sulfooxy)phenylacetic
acid (SPA), which are also proposed to exist in a dormant state prior to transformation to more active
desulfated metabolites. However, these two sulfated phenolic acids are most likely not involved in the
wound plug formation, but could be serving as biofilm inhibitor. In this study we investigated the
phytochemical composition of D. vermicularis extracts of different polarities. The coumarin composition
of this alga seems to be more complex than previously described, and hence we report on two novel
coumarins from D. vermicularis and their analysis via HPLC-MS and NMR. Coumarins are natural
benzopyrone derivatives with a variety of desirable pharmacological properties which are commonly
found in higher plants [15], however sulfated molecules are rather uncommon and beyond that D.
vermicularis is the first alga known to contain these secondary metabolites. Therefore, we report on
the first validated HPLC assay for the separation and quantification of sulfated coumarins in algae
which has been applied to samples from three different sampling sites across the Mediterranean Sea to
compare their coumarin content.

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2. Results
2.1. Isolation and Identification of the Coumarins
With the aim of isolating the major compounds present in Dasycladus vermicularis four coumarins
and two phenolic acids were isolated from the methanol and the aqueous extract. A sample (2.5 g) of
the aqueous extract was separated by means of repeated flash chromatography and semi-preparative
HPLC, which resulted in the isolation of compounds
from 4.5 g of crude methanol extract by silica gel and Sephadex LH 20 column chromatography. H-
and 13C-NMR shift values of all isolated coumarins are summarized in Table 1. The shift values for
1
,
4
and
8
. Compounds
2
,
3
and
7
were obtained
1
thyc were in good agreement with literature values [16]. Compounds
natural products.
1 and 2 were identified as new
Table 1. NMR shift values of compounds isolated from the marine green alga Dasycladus vermicularis;
spectra were recorded on a 600 MHz NMR instrument in deuterated water.
Dasycladin A (1) in D2O
Dasycladin B (2) in D2O
HMBC
C
HMBC
C
Position
δ
δC, Type
δ
δC, Type
H
H
2
3
4
5
6
7
8
9
10
163.3, C
136.1, C
134.4, CH
118.3, C
144.1, C
152.7, C
106.8, CH
150.2, C
113.5, C
163.0, C
135.8, C
135.8, CH
115.8, CH
145.7, C
151.5, C
111.2, C
147.7, C
113.9, C
161.0, C
134.1, C
133.3, CH
122.4, CH
137.6, C
153.0, C
105.1, CH
151.1, C
112.1, C
7.40 (s)
7.10 (s)
2, 3, 5, 9
7.94 (s)
7.64 (s)
2, 3, 5, 9
4, 6, 7, 9
6, 7, 9, 10
7.05 (s)
6, 7, 9, 10
0
2
3
4
5
6
7
8
9
0
0
0 0 0
2 , 3 , 5 , 9
0
0
7.96 (s)
7.28 (s)
0
0 0 0
4 , 6 , 7 , 9
0
0
0
0
0
10
Compound
1 was isolated as a yellowish amorphous powder. Its molecular formula was
established as C18H10O16S2 by HR-ESI–MS (m/z 544.934, calcd. for [C18H10O16S2-H]−, 544.936)
indicating the presence of a sulfated dicoumarin. Fragments at m/z 464.9 [M-H-80]− and 385.0
[M-H-80-80]− could be attributed to the loss of sulfate groups, respectively. The melting point was
measured to as 278–280 ◦C The IR spectra shows a strong and characteristic S=O stretching vibration
at about 1038 cm−1 for the R-OSO3H groups, 1689 and 1629 cm−1 for (C=O), 3059 and 3203 cm−1 (OH).
0
In the 1H-NMR (Table 1) spectrum, four singlet aromatic proton resonances at δH 7.96 (1H, s, H-4 ), 7.40
(1H, s, H-4), 7.28 (1H, s, H-5 ), 7.10 (1H, s, H-8) were observed. The 13C-NMR (Table 1) spectrum showed
0
eighteen carbon signals, which were assigned by DEPT experiments to four aromatic methines and
fourteen quaternary carbons, two of which (δC 163. 3 and 163.0) could be attributed to intramolecular
ester groups. The spectroscopic data suggested that
1 is composed of two identical coumarin moieties
substituted in positions C-3(C-30), C-6(C-60), and C-7 (C-70). The absence of corresponding signals
in the 1H-NMR spectrum and the low field-shifted C-5 and C-80 signals in the 13C-NMR spectrum
indicated that the two symmetric coumarin moieties were connected at 5–80 through a C-C bond.
Localization of the two sulfate groups was established based on the low field-shifted signals of C-3
and C-30 as well as by comparison of NMR data with those of thyc (
and HMBC experiments of
were useful to assign all signals in the 1H and 13C spectra. C-4 (
4
) [16] and compound
3
. HSQC
1
δ
134.4)

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and C-8 (
δ
106.8) were used to assign the H-4 (7.40, s) and H-8 (7.10, s) as well as C-40 (
δ
135.8) and
C-50 (δ 115.8) which were assigned to H-40 (7.96, s) and H-50 (7.28, s) by 1H-13C HSQC.
The HMBC spectrum showed key correlation peaks between the proton signal at δH
7.93 (1H, s, H-40) and carbon resonances at δC 163.0 (C-20), 147.7 (C-90), 135.8 (C-30), 115.8
(C-50); and between the proton signal at δH 7.38 (1H, s, H-4) and carbon resonances at
δC 0 163.3 (C-2), 150.3 (C-9), 136.1 (C-3), 118.3 (C-5). Thus, compound
1 was identified as
0
0
0
5,8 -di-(6(6 ),7(7 )-tetrahydroxy-3-sulfoxy-3 -sulfoxycoumarin), a new coumarin for which we propose
the trivial name “dasycladin A”.
Compound
2 was also isolated as a yellowish amorphous powder. The molecular formula
of C9H6O11S2 was determined by HR-ESI–MS (negative mode) with a mass peak at m/z 352.92−8
−
(calcd. for [C9H6O11S2-H] , 352.926). The mass spectrum showed a fragment at m/z 272.8 [M-H-80]
corresponding to the loss of a sulfate moiety. The melting point was measured as 233–238 ◦C. The IR
spectra shows a strong and characteristic S=O stretching vibration at about 1038 cm−1 for the R-OSO3H
groups, 1697 cm−1 for (C=O), 3217 cm−1 (OH). The 1H-NMR spectrum (Table 1) of
2 showed signals
for three aromatic protons at δH 7.94 (1H, s, H-4), 7.64 (1H, s, H-5), 7.05 (1H, s, H-8). In the 13C-NMR
(Table 1) spectrum nine carbon signals, including three aromatic methines and six quaternary carbons
were observed. HSQC and HMBC experiments of Compound
2
were useful to assign all signals in
105.2) were used to assign the
the 1H- and 13C-NMR spectra. C-4 (
δ
133.3), C-5 ( 122.4) and C-8 (δ
δ
protons H-4 (7.94, s), H-5 (7.64, s) and H-8 (7.05, s) by 1H-13C HSQC. The HMBC spectrum showed
correlation peaks between the proton signal at δH 7.94 (1H, s, H-4) and carbon resonances at δC 161.1
(C-2), 151.1 (C-9), 134.2 (C-3), 122.4 (C-5); between the proton signal at δH 7.64 (1H, s, H-5) and carbon
resonances at δC 152.9 (C-7), 151.1 (C-9), 137.7 (C-6), 133.3 (C-4); and between the proton signal at δH
7.04 (1H, s, H-8) and carbon resonances at δC 152.9 (C-7), 151.1 (C-9), 137.7 (C-6), 112.3 (C-10). These
spectroscopic data suggested that compound
signal of C-3 and the up field-shifted of C-6 indicated that the sulfate groups were attached at these
carbons. The structure of was deduced as 7-hydroxycoumarin-3,6-disulfate. Compound represents
2 is 3,6,7-trisubstituted coumarin. The low field-shifted
2
2
a new coumarin for which we propose the trivial name “dasycladin B”.
NMR spectra and HR-ESI–MS data for the novel sulfated coumarins
S1A–E and S2A–E in the Supplementary Material.
1
and 2 are shown in Figures
2.2. HPLC-Method Development
A HPLC method was developed for quantification of the coumarins. Four coumarins were isolated
as described above and used as standards in addition to the two synthetized sulfated phenolic acids
and their educts (see Figure 1). Several different stationary phases were screened for the separation
of the coumarins and phenolic acids in D. vermicularis, such as Zorbax SB-C18 3.5
ODS 3 m, YMC-triart C-18, 3.5 m and Kinetex C-18 2.6 m. However, the best separation was
achieved on the Gemini C 18 110 Å, 3 m (150 mm 4.6 mm). The latter column yielded the best
µm, Hyperclone
µ
µ
µ
µ
×
results concerning separation efficiency and peak shape, resulting in an optimum separation within
less than 25 min (Figure 2).
5,80-Di-(6(60),7(70)-tetrahydroxy-3-sulfoxy-30-sulfoxycoumarin) (
followed by 7-hydroxycoumarin-3,6-disulfate (2;
1
) eluted first (13.12 min),
16.58 min), the sulfated phenolic acids
4-(sulfooxy)benzoic acid and 4-(sulfooxy)phenylacetic acid ( 17.73 min, 18.3 min),
then 6,7-dihydroxycoumarin-3-sulfate ( ; 18.81 min), 3,6,7-trihydroxycoumarin ( ; 20.63 min),
4-(hydroxyl)phenylacetic acid (7; 21.62 min), and finally 4-(hydroxyl)-benzoic acid (8; 22.07 min).
5;
6;
4
3

Molecules 2018, 23, 2735
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Figure 1. Chemical structure of the isolated coumarins from the marine green alga Dasycladus
vermicularis, the sulfated phenolic acids and their educts.
2.3. Method Validation
The new analytical method was validated according to the ICH guidelines [17] by establishing
calibration curves of the two coumarin standards
acid and 4-hydroxyphenylacetic acid and their sulfated products 4-(sulfooxy)benzoic acid (SBA) and
4-(sulfooxy)phenylacetic acid (SPA) . Sufficient material was not available for and . Excellent
determination coefficients (R2
0.9986) were obtained within a concentration range of 0.859–1154 g/mL.
1 and 4, as well as the phenolic acids 4-hydroxy-benzoic
5–8
2
3
≥
µ
Individual calibration levels were obtained by serial dilution, and each solution was analyzed under
optimum HPLC conditions in triplicate. Limit of detection (LOD) and limit of quantification (LOQ) were
calculated using defined concentration equivalents to S/N ratios of 3 (LOD) and 10 (LOQ). LOD and LOQ
values ranged from 0.014–1.939 µg/mL, and from 0.044–5.876 µg/mL, respectively (Table 2). Selectivity
of the method was assured by no visible co-elution (shoulders) in the relevant signals, LC-MS data, and
by very consistent UV-spectra (as confirmed by the peak purity option in the operating software). The
methods precision was confirmed by its repeatability, as well as inter- and intra-day variation which were
determined in D. vermicularis sample (DV-2). For this purpose five individual samples at 250 mg/25 mL
were extracted and analyzed on each of three consecutive days (Table 3). Intra-day (RSD
≤
5.99%)
and inter-day precision (RSD 7.49%) were within accepted limits. Accuracy was assured by spiking
≤
accurately weighed samples of DV-2 with three different concentrations of the standard substances. For
all compounds the observed recovery rates were acceptable and ranged from 95.6 to 104.6%. Only for
3,6,7-trihydroxcoumarin the value for the low spike was at 91.3% (Table 3). This compound appeared to
be stable in solution for at least several h, however when added to the extract it degrades rapidly and
therefore the recovery rates are poor, especially at the lower concentrations.

Molecules 2018, 23, 2735
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Figure 2. HPLC separation of all standards
1 to 8 at 254 nm and all coumarins 1 to 4 isolated from the
marine green alga Dasycladus vermicularis at 350 nm, and the sample DV-1 from the marine green alga
Dasycladus vermicularis at 254 nm and 350 nm under optimized conditions (column: Gemini C18, 110 Å
column (150
×
4.6 mm, 3 µm); mobile phase (A) aqueous 20 mM ammonium acetate solution with
1.5% acetic acid and (B) methanol/water (9:1) with 20 mM ammonium acetate and 1.5% acetic acid.
Gradient: 2% B to 15% B from 0 to 5 min and 15% B to 60% B from 5–20 min and 60% B to 98% B from
20–25 min; detection at 254 nm and 350 nm, flow rate 0.3 mL/min, injection volume 5
oven temperature. Peak assignment is according to Figure 1.
µ
L and 40 ◦C

Molecules 2018, 23, 2735
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Table 2. Calibration data for the coumarins isolated from the marine green alga Dasycladus vermicularis and the respective phenolic acids.
Parameter
1 (Dasycladin A)
4 (thyc)
5 (SBA)
6 (SPA)
7 (4-OH-PAA)
8 (4-OH-BA)
Regr. Equation
σ rel of the slope
R
Y = 22.953x − 7.904
Y = 67.354x − 296.52
Y = 19.684x + 22.158
0.116
Y = 0.652x − 1.475
Y = 97.057x + 14.771
0.049
Y = 2.110x + 2.757
0.339
0.09
0.683
0.9986
445–6.953
0.589
0.175
0.9999
440–0.859
0.192
0.581
0.9999
629–1.229
0.039
0.117
1.000
1154–18.031
1.000
124.75–0.975
1.000
483–3.770
1.045
3.168
Range (µg/mL)
LOD 1
1.939
5.876
0.014
0.044
LOQ 2
1.784
1 LOD: limit of detection determined with purified standards (in µg/mL). 2 LOQ: limit of quantification determined with purified standards (in µg/mL).
Table 3. Accuracy and precision of the new coumarin assay.
Accuracy 1
Medium Spike
Precision 2
Substance
High Spike
99.49
-
Low Spike
101.89
-
Day 1
7.49
2.22
6.39
-
-
-
-
-
Day 2
1.57
4.43
4.37
Day 3
1.38
6.65
2.81
Intra-Day
1
2
3
4
5
6
7
8
95.63
-
-
97.87
99.58
103.23
104.06
99.50
2
1.86
3.71
5.99
-
-
-
-
-
-
-
97.49
102.62
98.70
97.45
102.10
91.34
99.40
100.72
103.77
98.85
-
-
-
-
-
-
-
-
-
-
1
Expressed as recovery rates in percent (sample DV-2 Alonissos). maximum relative standard deviation (peak area) within one and three consecutive days (n = 5; sample: DV-2).

Molecules 2018, 23, 2735
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2.4. Analysis of Samples
Four different samples of D. vermicularis, all originating from the Mediterranean Sea, were
analyzed for their coumarin content. For further details on collection sites, seasons and water depths
see Supplementary Material Table S1. As expected, the total amount of coumarins in sample DV-2
(1.60–1.80 m depth) was much lower (3.66 mg/g DW) compared to sample DV-3 (0.30–0.80 m depth;
10.17 mg/g DW). The quantity of the monomeric sulfated coumarins
2 and 3 was the same, while the
content of the dimeric compound was about three times higher in the sample that was collected from
1
shallow waters. The two samples that were collected from the same spot in Volos, Greece (DV-3 and
DV-4) indicated that there might be seasonal changes in the coumarin content of D. vermicularis as well.
In November (sample DV-4; 4.27 mg/g DW), the total coumarin content was only half the amount
as in August (sample DV-3; 10.17 mg/g DW). The sample that was harvested from Malaga, Spain
(DV-1) showed the highest amount of coumarins with 16.09 mg/g DW. The dimeric coumarin
the most abundant compound in all samples, followed by , the 6,7-dihydroxycoumarin-3-sulfate, and
7-hydroxycoumarin-3,6-disulfate ( ). Thyc, which is reported to be the sole metabolite of compound 3,
1 was
3
2
only occurred in traces in the highest concentrated samples DV-1 and DV-3. Phenolic acids were also
present in all samples, however, in much lower content. All samples contained 4-hydroxybenzoic acid
and at a higher concentration its sulfated precursor 4-(sulfooxy)benzoic acid. All quantitative results
are summarized in Figure 3. 4-(Sulfooxy)phenylacetic acid which was reported to be present in D.
vermicularis in a previous publication by Kurth et al. [14] could not be detected in the samples by DAD;
however, LC-MS revealed the presence of this compound in all samples. In addition to the standard
compounds, three other signals were tentatively identified as coumarins based on the typical UV and
MS-spectra; they are marked with a star (Figure 4). The compounds a* and b* both have UV absorption
−
maxima of 346 nm and m/z of 465 [M-H ]. These two compounds may be monosulfated dicoumarins.
The assignment of other signals was easily possible by comparison to standards. For example, the
determination of coumarins in sample DV-1 is shown in Figure S5 in the Supplementary Material.
Chromatograms were recorded at 254 nm and 350 nm, the other traces show the identification of
individual compounds by LC-MS in EIC mode.
(A)
Figure 3. Cont.

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(B)
dasycladin A, (2) dasycladin B, (extbf3)
Figure 3.
Quantification of the coumarins
(
1
)
6,7-dihydroxycoumarin-3-sulfate, (4) 3,6,7-trihydroxycoumarin. Concentrations are given as mg coumarins/g
dry weight (
A
) and as mg/g phenolic acids as dry weight ( ) (n = 3) in the green alga Dasycladus vermicularis
B
collected at different sampling sites and dates in the Mediterranean Sea.
Figure 4. Base peak chromatograms of all extracts DV1-DV3 prepared from the marine green alga
Dasycladus vermicularis. The red area is enlarged and shows the extracted ion chromatograms (EIC) for
the sulfated phenolic acids SBA (
5) and SPA (6). Tentatively identified minor coumarins are marked
with a star (similar UV spectra) and with a* and b* (similar UV spectra and molecular weight).

Molecules 2018, 23, 2735
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3. Discussion
The chemical composition of coumarins and phenolic acids in the marine green alga D. vermicularis
was investigated in detail in this study, revealing that the coumarin composition is more complex
than previously reported [8]. Dasycladin A is the major compound, which probably degrades to the
previously reported 6,7 dihydroxycoumarin-3 sulfate. Similarly, it can be speculated that dasycladin B
is metabolized to the corresponding monosulfated coumarin first and afterwards to the more active
metabolite 3,6,7-trihydroxycoumarin, since the sulfated compounds have been reported as dormant
forms which are enzymatically transferred into active metabolites through sulfatases [9]. The sulfated
phenolic acids SPA and SBA were both present in the extract, however at very low concentrations.
These findings are highlighted with red in Figure 4 and are in good agreement with the LC-MS data
published by Kurth et al. [14]. Coumarins in general show a wide range of different pharmacological
activities, including anti-HIV, anti-tumor, anti-hypertensive, anti-coagulant, anti-inflammatory just
to name a few. They have become important lead compounds in drug research due to their high
bioavailability, low molecular weight, and low toxicity [15,18]. For simple coumarins anti-oxidative
activity has been reported especially for compounds with free hydroxyl groups. Likewise, the C-7 free
hydroxyl group is important for anti-bacterial activity and is also important for an anti-inflammatory
activity [19]. The C-6 free hydroxyl group is important for both anti-bacterial and anti-fungal activity.
A free hydroxyl group in position 3 (e.g., Compound
strongly enhanced inhibition of 5-Lipoxygenase and
4
in this study) is especially important for a
α
-D-glucosidase [20]. Sulfated coumarins are
rather uncommon but due to their negative charge they could bind to heparin receptors or inhibit
platelet aggregation [21].
Besides recently developed HPLC-DAD, LC-MS and SFC methods for the separation of furo-
pyrano- and monocoumarins on reversed phase columns [22–24], this study presents the first method
for the separation of the definitive more polar sulfated coumarins. Their content was found to be
quite variable in the different samples suggesting that water depth and seasonal changes including
fluctuations in the visible and ultraviolet part of solar radiation might have a strong influence.
D. vermicularis has been reported to excrete UV-absorbing brown-green substances under in-situ
conditions staining the nearby seawater and thus being beneficial as photo-protective compounds
for other macroalgae living in the vicinity [
coumarins and since they are accumulated in the outer parts of the siphonous cell walls, particularly
after UV exposure, they are considered to act as UV-sunscreens [ ]. 3,6,7-trihydroxycoumarin (thyc)
6]. The responsible compounds were later identified as
6
was found to be preferentially localized in the apical part of the D. vermicularis thallus, which usually
experiences highest natural insolation, particularly in the internal part of the cell wall and around
the tonoplast. The percentage of UVR absorbed by both thyc layers could be measured from the
in-vitro total thallus concentration of thyc and histological measurements of these layers. While
the cell wall thyc layer absorbed 88% of the incident UVR irradiance, the one close to the vacuole
membrane absorbed a similar fraction with 87.5% [6]. These data strongly support the hypothesis
of coumarins/phenolics as natural sunscreen compounds reducing biologically harmful UVR from
reaching sensitive biomolecules in the cell such as DNA and proteins. Phenolic compounds also
play an important role in the interaction of macroalgae with their environment. They are relevant for
different supporting or protective tissues, for example in cell wall formation, they can be involved
in defense mechanisms, for example in anti-herbivory or having antibacterial effects, and signaling
properties, for example in allelopathy [25].
The conspicuous decrease of coumarin content in November could make D. vermicularis more
susceptible to abiotic and biotic stressors, but UVR and biotic interactions are less strong in late
autumn compared to summer in the Mediterranean Sea. However, a larger number of samples
from different origins, seasons and water depths is definitely needed to examine the qualitative and
quantitative metabolic composition of D. vermicularis and its chemical reaction after wounding or other
stress situations.

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4. Materials and Methods
4.1. Reagents and Chemicals
All solvents used for isolation, synthesis and analytical studies were of analytical grade and
purchased from Merck (Darmstadt, Germany). HPLC grade water was produced by a Sartorius arium
611 UV water purification system (Sartorius Göttingen, Germany). Reagents for the synthesis of
4-(sulfooxy)benzoic acid and 4-(sulfooxy)phenylacetic acid (4-hydroxybenzoic acid, sulphur trioxide
pyridine complex (Pyr*SO3), and 4-hydroxyphenylacetic acid) were purchased from Sigma Aldrich
(Taufkirchen, Germany). Deuterated water for NMR experiments was obtained from Euriso-top
(Saint-Aubin Cedex, France).
4.2. Algal Material
Four samples of Dasycladus vermicularis were analyzed. DV-1(330 g dry weight) was collected in
September 1998 from the upper part of the infralittoral zone (0.5 m depth) in the Cabo de Gata-Nijar
Natural Park (36◦ 520 N, 2◦ 120 W, Almerıa, Southern Spain) and identified by Prof. Felix Figueroa
(University of Malaga), freeze-dried and sent to Ulf Karsten, who stored the material under cool, dry
and dark conditions prior to further processing in Innsbruck. Sample DV-2 was harvested in August
2017 in Alonissos, Greece (39◦08023.800 N, 23◦50043.300 E) at 1.80 m depth; Sample DV-3 and DV-4
were both harvested in Volos, Greece (39◦1900.000 N, 23◦01011.500 E), DV-3 in August 2017 and DV-4
in November 2017 at 30–80 cm depth. All samples from Greece were collected and identified by the
author (A. Hartmann). All samples were air-dried and voucher specimen of all samples are stored at
the Institute of Pharmacy, Pharmacognosy, University of Innsbruck.
4.3. Instrumentation
NMR experiments were conducted on an Avance II 600 spectrometer (Bruker, Karlsruhe, Germany)
operating at 600.19 (1H) and 150.91 MHz (13C). Spectra of the respective compounds were recorded
in deuterated solvents from Euriso-Top adding 3-(trimethylsilyl)-propionic acid sodium salt (TMSP)
as an internal standard. Infrared (IR) spectra were recorded on an ALPHA Fourier transform (FT)-IR
apparatus (Bruker, Billerica, MA, USA) equipped with a platinum attenuated total reflection module.
Analytical HPLC experiments were carried out on an Agilent 1100 system (Agilent, Waldbronn,
Germany) equipped with a binary pump, autosampler, diode array detector and column oven. For the
purification of the compounds a semi-preparative HPLC from Dionex (ThermoFisher, Waltham, MA,
USA), comprising of a HPG-3200 pump, a VWD-3100 detector, column oven and a fraction collector
was utilized. Additionally, the exact mass of the novel compounds 1 and 2 were determined in negative
ESI mode on a micrOTOF-Q II MS (Bruker, Bremen, Germany). The settings were: nebulizer gas:
4.4 psi, dry gas: 4 L/min, dry temperature: 180 ◦C, capillary voltage: 2.5 kV, set capillary V 3500, set
endplate offset V-500.
4.4. Isolation and Structural Analysis of Coumarins
Dried algal material of Dasycladus vermicularis (300 g, DV-1, Malaga), were finely ground to powder
and subsequently extracted five times with 100% dichloromethane (p.a.) for 15 min in an ultrasonic
bath. After centrifugation at 1537
×
g, the combined solutions were evaporated to dryness at 40 ◦C
under reduced pressure to yield 1.13 g of crude dichloromethane extract (DV-1D). The plant material
was subsequently extracted with 100% methanol (p.a.) using the same procedure to yield 4.71 g of crude
methanol extract (DV-1M). The algal residue was extracted for a third time using the same procedure
with a 1:1 mixture of water and methanol to yield 7.9 g crude aqueous extract. The crude methanol
extract (4.5 g) was separated into 10 fractions (DV-M-S1-10) on silica gel (40–63 µm particle size) using
a dichloromethane/ethyl acetate/methanol/water gradient. Fraction DasyM-S7 was further purified
using size-exclusion chromatography on Sephadex LH-20 material with methanol:water (1:1) as eluent
to obtain 16 subfractions (DV-M 7.1-16). This resulted in the isolation of compounds
2 (5.24 mg) and 3

Molecules 2018, 23, 2735
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(4.04 mg). A portion (2.5 g) of the crude aqueous extract was first separated using flash chromatography
(on RP-18 material (80 g Reveleris cartridge, 40 m) and a water/methanol gradient, containing 0.25%
formic acid in each solvent. 20 subfractions were obtained (DV-W-R1-20). Fraction 18 resulted in the
pure compound . DV-W-R6 (39.56 mg) was purified by semi-preparative HPLC on a Lichrosorb RP-18
(250 10 mm, 7 m) column with a gradient of 2–50 % methanol in water within 25 min at a flow of
1 mL/min. The oven temperature was set to 20 ◦C and the UV-detector signal to 350 nm, resulting
in 7.91 mg of compound . Fraction DV-W-R14 (123 mg) was re-chromatographed on a smaller C18
column using flash chromatography which resulted in 2.6 mg of compound . The samples were
µ
4
×
µ
1
8
dissolved in deuterated water with sodium trimethylsilyl propionate (TSP) as internal standard and in
case of 3,6,7-trihydroxycoumarin deuterated methanol was used. NMR data of the isolated coumarins
are summarized in Table 1. The shift values for thyc were in good agreement to literature values [16].
NMR spectra for the novel sulfated coumarins
the Supplementary Material. Compounds and
identified by comparison of their reported spectroscopic data, including ESI-MS and NMR data:
1
and
2 are shown in Figure 1A–D and Figure 2A–D in
3
4
are known natural products. Their structures were
6,7-Dihydroxycoumarin-3-sulfate (
3
, Figure 1): UVmax 268, 346 nm. It was assigned with a molecular
1
formula C9H5O8S of ESI-MS, m/z 272.98 [M
7.87 (s, 1H), 7.10 (s, 1H), 6.95 (s, 1H) 13C NMR
145.1 (C-6), 151.8 (C-7), 106.0 (C-8), 149.7 (C-9), 114.2 (C-10) [9].
−
H]−. H-NMR (600 MHz, D2O + 0.05% TSP)
δ
(ppm):
δ
(ppm): 163.6 (C-2), 135.9 (C-3), 135.8 (C-4), 115.7 (C-5),
3,6,7-Trihydroxycoumarin ( , Figure 1): UVmax 268, 346 nm. It was assigned with a molecular formula
4
−
1
C9H6O5 of ESI-MS, m/z 193.14 [M
−
H] . H-NMR (600 MHz, D2O + 0.05% TSP)
δ(ppm): 6.94 (s, 1H),
6.79 (s, 1H), 6.73 (s, 1H) 13C-NMR
δ (ppm): 161.7 (C-2), 140.3 (C-3), 117.6 (C-4), 111.6 (C-5), 144.5 (C-6),
148.0 (C-7), 103.5 (C-8), 145.3 (C-9), 113.9 (C-10) [16].
4.5. Sample Preparation
The powdered dried alga (200 mg) was extracted three times with 8 mL of water: methanol
(1:1) each by 15 min of sonication (Sonorex 35 KHz, Bandelin, Berlin, Germany). After centrifugation
(
1000× g for 3 min), the supernatants were combined in a 25 mL volumetric flask. Samples were
measured immediately after extraction.
4.6. Analytical Conditions
Experiments were performed on an Agilent 1100 HPLC system using a Gemini C18, 110 Å column
(150
×
4.6 mm, 3 µm) from Phenomenex (Aschaffenburg, Germany). The mobile phase (A) contained
water with 20 mM ammonium acetate and 1.5% acetic acid and (B) methanol/water (9:1) with 20 mM
ammonium acetate and 1.5% acetic acid. Elution was performed in gradient mode starting with 2% B to
15% B from 0 to 5 min, 15% B to 60% B from 5–20 min and 60% B to 98% B from 20–25 min, followed by
10 min of re-equilibration with 98% A. The DAD was set to 254 nm and 350 nm, and flow rate, sample
volume and column temperature were adjusted to 0.3 L/min, 5 µ
L and 40 ◦C, respectively. HPLC-MS
experiments were carried out on an Agilent 1260 HPLC system coupled to an amaZon iontrap mass
spectrometer (Bruker, Bremen, Germany). The chromatographic conditions were as described before;
◦
MS-spectra were recorded in negative ESI mode, with a drying gas temperature of 220 C, the nebulizer
gas (nitrogen) set to 23 psi, and a nebulizer flow (nitrogen) of 6 L/min. The scanned mass range was
between m/z 70–1500, at a capillary voltage of 4.5 kV.
4.7. Synthesis of 4-(sulfooxy)benzoic Acid and Synthesis of 4-(sulfooxy)phenylacetic Acid
The synthesis of the two sulfated phenolic acids
5 and 6 was carried out as described recently
by Kurth et al. [14], however using smaller quantities. 4-Hydroxybenzoic acid (1.5 g), sulfur trioxide
pyridine complex (Pyr∗SO3, 1.72 g) were dissolved in water free pyridine (25 mL) and stirred in a
◦
250 mL round bottomed flask at 25 C for 48 h. For the synthesis of SPA, 4-hydroxyphenylacetic
acid (0.75 g) and sulfur trioxide pyridine complex (Pyr∗SO3, 0.83 g) were used. Subsequently, the

Molecules 2018, 23, 2735
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◦
pyridine was removed by evaporation at 40 C under reduced pressure. The remaining yellow-brown
oil was dissolved in water (20 mL, HPLC grade) and the solution adjusted to pH 6–7 (pH-Meter,
Mettler Toledo, Greifensee, Switzerland) using 25% potassium hydroxide. The aqueous solution was
washed three times in a separatory funnel. A white precipitate was formed and filtered off after phase
◦
separation. Subsequently, the clear aqueous solution was evaporated at 45 C under reduced pressure
to give a white residue, which was re-dissolved in water and adjusted to pH 10 again using 25% KOH.
◦
To cleave the anhydride side products this solution was stirred at 60 C for 1 h and subsequently
neutralized using diluted sulfuric acid◦and afterwards evaporated again. The yellow-white residue
was suspended in 10 mL water at 40 C (5 mL for the synthesis of SPA). A white precipitate was
formed by adding 20 mL (10 mL) of methanol, which was filtered off and sub◦sequently washed with
10 mL (5 mL) of methanol. The methanol fractions were combined and left at 4 C for 24 h in the fridge,
so that a crystalline precipitate was formed; it was filtered off and the solution evaporated to dryness.
The so obtained raw product was suspended in the ultrasonic bath using 2 mL (1 mL) methanol
(HPLC-grade), which resulted in a white powder in a yellow solution. This step was repeated twice.
Finally the product was washed with acetone and dried at 70 ◦C to give 660.93 mg (44% yield) SBA
and 253.86 mg SPA (33.8% yield).
SBA: 1H-NMR (600 MHz, D2O + 0.05% TSP)
δ(ppm): 7.91 (d, 2H; J = 8.4 Hz); 7.36 (d, 2H; J = 8.4 Hz),
13C-NMR
δ(ppm): 177.7(C, C-1), 123.9 (C, C-2), 136.8 (CH,C-3,C-7), 133.3 (CH, C-4,C-6), 156.4 (C, C-5)
HPLC–MS m/z [M − H]−: 216.99
SPA: 1H-NMR (600 MHz, D2O + 0.05% TSP)
δ(ppm): 3.55 (s, 2H; J = 8.4 Hz), 7.26 (d, 2H; J = 8.5 Hz),
7.32 (d, 2H), 13C-NMR
δ(ppm): 183.5 (C, C-1), 46.5 (CH2, C-2), 124.2 (C, C-3), 133.1 (CH,C5; CH, C7),
138.1 (CH,C4; CH,C8), 152.3 (C, C-6), HPLC–MS m/z [M − H]−: 231.00.
4.8. Synthesis of 3,6,7-trihydroxycoumarin
The synthesis of thyc was performed as previously published by Cotelle et al. [26] Briefly, 2,4,5
trihydroxybenzaldehyde (2.31 g), acetylglycine (2.10 g) and sodium acetate (1.59 g) were weighted
into a round bottomed flask (100 mL), acetic anhydride (7.5 g) was added and the mixture was
◦
heated under reflux for 4 h (130 C). The solution was cooled to room temperature and ice water
(10 mL) was added. The precipitate was filtered, washed with ethanol/water (50:50) and dried to give
3-acetamido-6,7-diacetoxycoumarin. A solution of this intermediate (1.59 g) in 3 M HCl (50 mL) plus
acetic acid (2 mL) was refluxed for 1 h and subsequently cooled to room temperature. The obtained
precipitate was washed again with water to give pure 3,6,7-trihydroxycoumarin (667.2 mg).
Supplementary Materials: The following are available online. Table S1: Origin of analyzed samples,
Figure S2A–E: NMR spectra and HR-ESI-MS spectra of Compound 1, Figure S3A–E: NMR spectra and
HR-ESI-MS spectra of Compound 2, Figure S4: UV spectra of the 4 Coumarins recorded on line by DAD,
Figure S5: Determination of coumarins ( : dasycladin A, : dasycladin B, : 6,7-dihydroxycoumarin-3sulfate,
3,6,7-trihydroxycoumarin) in the marine green alga Dasycladus vermicularis.
1
2
3
4:
Author Contributions: A.H. did all analytical work, isolated/elucidated the coumarins and wrote the manuscript;
M.G. aided in analytical questions and corrected the manuscript; U.K. supplied and identified the algae and
edited the first draft. A.S. aided in the structure elucidation of the novel coumarins.
Funding: This work was financially supported by the Austrian Science Fund (FWF), project P296710 and EMBO
(ASTF 448-2016).
Acknowledgments: We thank Barbara Matuszczak for measuring the IR spectra.
Conflicts of Interest: The authors declare no conflict of interest.

Molecules 2018, 23, 2735
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Sample Availability: Samples of the compounds/fractions are available from the authors upon request.
©
2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access
article distributed under the terms and conditions of the Creative Commons Attribution
(CC BY) license (http://creativecommons.org/licenses/by/4.0/).