Genetic breeding of L-tyrosine producer from Brevibacterium lactofermentum.

Article abstract of DOI:10.1080/00021369.1990.10870028

A wild-type parent of Brevibacterium lactofermentum was converted into an L-Tyr producer by three steps of genetic breeding. First, acquirement of m-fluoro-D, L-phenylalanine resistance (1,000 microgram/ml) brought about MF1317 which produced 3.5 g/l of L-Tyr and a byproduct of 2.8 g/l of L-Phe. Second, increase in the drug resistance (5,000 microgram/ml) gave MF358 that produced 6.4 g/l of L-Tyr and a byproduct of 6.0 g/l of L-Phe. Third, an L-Phe auxotrophic mutant (FT-1) derived from MF358 accumulated 16 g/l of L-Tyr. In FT-1, L-Phe was not accumulated at all, but a small amount of anthranilate (0.4 g/l) was. A key enzyme in the biosynthesis of L-Tyr, 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, was free from synergistic feedback inhibition by L-Tyr and L-Phe in the producers, and so L-Tyr accumulation occurred independently of L-Phe concentration in the production medium.

Full text of DOI:10.1080/00021369.1990.10870028

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Genetic Breeding of l-Tyrosine
Producer from Bvevihactevium
lactofermentum
Hisao Itoa, Shoji Sakuraia, Takashi Tanakaa, Katsuaki Satoa &
Hitoshi Eneia
a Central Research Laboratories of Ajinomoto Co., Inc., 1-1
Suzuki-cho, Kawasaki-ku, Kawasaki 210, Japan
Published online: 08 Sep 2014.
To cite this article: Hisao Ito, Shoji Sakurai, Takashi Tanaka, Katsuaki Sato & Hitoshi Enei (1990)
Genetic Breeding of l-Tyrosine Producer from Bvevihactevium lactofermentum, Agricultural and
Biological Chemistry, 54:3, 699-705
To link to this article: http://dx.doi.org/10.1080/00021369.1990.10870028
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