Agricultural and biological chemistry p. 699 - 705 (1990)
Update date:2022-08-02
Topics:
Ito
Sakurai
Tanaka
Sato
Enei
A wild-type parent of Brevibacterium lactofermentum was converted into an L-Tyr producer by three steps of genetic breeding. First, acquirement of m-fluoro-D, L-phenylalanine resistance (1,000 microgram/ml) brought about MF1317 which produced 3.5 g/l of L-Tyr and a byproduct of 2.8 g/l of L-Phe. Second, increase in the drug resistance (5,000 microgram/ml) gave MF358 that produced 6.4 g/l of L-Tyr and a byproduct of 6.0 g/l of L-Phe. Third, an L-Phe auxotrophic mutant (FT-1) derived from MF358 accumulated 16 g/l of L-Tyr. In FT-1, L-Phe was not accumulated at all, but a small amount of anthranilate (0.4 g/l) was. A key enzyme in the biosynthesis of L-Tyr, 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, was free from synergistic feedback inhibition by L-Tyr and L-Phe in the producers, and so L-Tyr accumulation occurred independently of L-Phe concentration in the production medium.
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Doi:10.1016/0008-6215(93)84265-8
(1993)Doi:10.1002/ejic.201900618
(2019)Doi:10.1039/a901798d
(1999)Doi:10.1016/S0040-4039(02)02433-4
(2002)Doi:10.1021/ja00746a051
(1971)Doi:10.1055/s-1984-30966
(1984)