3 Diazoquinoline tetrafluoroborate as a reagent for specific modification of lysyl residues: unique structure of the reaction product

Article abstract of DOI:10.1016/0005-2795(74)90117-2

3 Diazoquinoline tetrafluoroborate can modify some of lysyl residues of proteins, such as trypsin, lysozyme and insulin, at pH 8.2 and 4° C. Tyrosyl and histidyl residues are not reactive to this diazonium salt. The modified lysyl residue was revealed to have a structure of 1 substituted 1,2,3 triazolo [4,5 c] quinoline, quite different from those of the usual azo coupling products.