Organocatalytic asymmetric assembly reactions: one-pot synthesis of functionalized beta-amino alcohols from aldehydes, ketones, and azodicarboxylates.

Article abstract of DOI:10.1021/ol034333n

[reaction: see text] l-Proline catalyzed the enzyme-like direct asymmetric assembly of aldehydes, ketones, and azodicarboxylic acid esters to provide optically active beta-amino alcohols. This assembly reaction uses both aldehydes and ketones as donors in

Full text of DOI:10.1021/ol034333n

ORGANIC
LETTERS
2003
Vol. 5, No. 10
1685-1688
Organocatalytic Asymmetric Assembly
Reactions: One-Pot Synthesis of
Functionalized â-Amino Alcohols from
Aldehydes, Ketones, and
Azodicarboxylates
Naidu S. Chowdari, D. B. Ramachary, and Carlos F. Barbas, III*
The Skaggs Institute for Chemical Biology and the Department of Molecular Biology,
The Scripps Research Institute. 10550 North Torrey Pines Road,
La Jolla, California 92037
carlos@scripps.edu
Received February 25, 2003
ABSTRACT
L-Proline catalyzed the enzyme-like direct asymmetric assembly of aldehydes, ketones, and azodicarboxylic acid esters to provide optically
active â-amino alcohols. This assembly reaction uses both aldehydes and ketones as donors in one pot. The aldol-derived stereocenter is
formed with a reduced facial selectivity in reactions involving (R)-amino aldehydes. The reactions can be performed on a multigram scale
under operationally simple and safe conditions without the requirement of an inert atmosphere or dry solvents.
Directed asymmetric assembly of simple achiral building
blocks into stereochemically complex molecules has long
been the purview of nature’s enzymes.1,2 In organic synthesis,
catalysts are traditionally designed and optimized to mediate
a single reaction. However, the increasing demand for
expedient and efficient synthetic processes requires the
development of catalysts that are capable of catalyzing
multiple reactions in a single pot. The use of multifunctional
catalysts not only generates less waste but ideally obviates
the tedious separation and purification of intermediate
products.3 Proline has been found to be effective for enamine-
based direct catalytic asymmetric aldol,4 Mannich,5 Michael,6
Diels-Alder,7 and R-amination reactions.8 Recently, we
described proline-catalyzed asymmetric assembly of poly-
ketides from three aldehyde substrates involving two con-
secutive aldol reactions in a single pot.4c,9 In a continuation
of our studies of amine-catalyzed aldol reactions,4,9 we aimed
to explore various highly functionalized aldehydes as ac-
(3) (a) Yu, H.-B.; Hu, Q.-S.; Pu, L. J. Am. Chem. Soc. 2000, 122, 6500.
(b) Weatherhead, G. S.; Ford, J. G.; Alexanian, E. J.; Schrock, R. R.;
Hoveyda, A. H. J. Am. Chem. Soc. 2000, 122, 1828. (c) Yamasaki, S.;
Kanai, M.; Shibasaki, M. J. Am. Chem. Soc. 2001, 123, 1256. (d) Louie,
J.; Bielawski, C. W.; Grubbs, R. H. J. Am. Chem. Soc. 2001, 123, 4609.
(e) Choudary, B. M.; Chowdari, N. S.; Madhi, S.; Kantam, M. L. Angew.
Chem., Int. Ed. 2001, 40, 4620. (f) Du, H.; Ding, K. Org. Lett. 2003, 5,
1091.
(4) (a) List, B.; Lerner, R. A.; Barbas, C. F., III. J. Am. Chem. Soc. 2000,
122, 2395. (b) Saktihvel, K.; Notz, W.; Bui, T.; Barbas, C. F., III. J. Am.
Chem. Soc. 2001, 123, 5260. (c) Co´rdova, A.; Notz, W.; Barbas, C. F., III.
J. Org. Chem. 2002, 67, 301. (d) Northrup, A. B.; MacMillan, D. W. C. J.
Am. Chem. Soc. 2002, 124, 6798. (e) Bogevig, A.; Juhl, K., Kumaragu-
rubaran, N.; Jorgensen, K. A. Chem. Commun. 2002, 620. (f) Bahmanyar,
S.; Houk, K. N.; Martin, H. J.; List, B. J. Am. Chem. Soc. 2003, 125, 2475-
2479.
(5) (a) Notz, W.; Sakthivel, K.; Bui, T.; Barbas, C. F., III. Tetrahedron
Lett. 2001, 42, 199. (b) Co´rdova, A.; Notz, W.; Zhong, G.; Betancort, J.
M.; Barbas, C. F., III. J. Am. Chem. Soc. 2002, 124, 1842. (c) Co´rdova,
A.; Watanabe, S.-i.; Tanaka, F.; Notz, W.; Barbas, C. F., III. J. Am. Chem.
Soc. 2002, 124, 1866. (d) List, B.; Pojarliev, P.; Biller, W. T.; Martin, H.
J. J. Am. Chem. Soc. 2002, 124, 827.
(1) Khosla, C. J. Org. Chem. 2000, 65, 8129.
(2) Gijsen, H. J. M.; Qiao, L.; Fitz, W.; Wong, C.-H. Chem. ReV. 1996,
96, 443.
10.1021/ol034333n CCC: $25.00 © 2003 American Chemical Society
Published on Web 04/22/2003

ceptors. Accordingly, we have studied amino aldehydes as
acceptors. Herein, we report L-proline-catalyzed direct asym-
metric assembly reactions involving three different compo-
nents, namely, aldehydes, ketones, and azodicarboxylic acid
esters, to provide optically active functionalized â-amino
alcohols in an enzyme-like assembly process. To the best of
our knowledge, these are the first examples of assembly
reactions that use directly both aldehydes and ketones as
donors in one pot.
Table 1. Proline-Catalyzed Asymmetric Assembly Reaction of
Acetone, Propionaldehyde, and Dibenzyl Azodicarboxylate in
Various Solvents
To develop the targeted assembly reaction, we first studied
the L-proline-catalyzed aldol reaction of acetone with (R)-
amino aldehyde (1). To our surprise the aldol product was
formed with reduced facial selectivity as compared to that
observed in other known proline-catalyzed aldol reactions
(Scheme 1). Since amino aldehydes such as 1 are accessible
entry
solvent
yield (%) dr (anti:syn) eea (%) (anti/syn)
1
2
3
4
6
5
7c
8d
DMSO
DMF
72
79
75
77
68
80
82
80
13:87
28:72
62:38
44:56
55:45
56:44
28:72
43:57
>99/74 (>99/98)b
>99/65
>99/49
>99/49
>99/28
>99/54
98/78
99/55
CH2Cl2
dioxane
acetone
CH3CN
CH3CN
CH3CN
Scheme 1. L-Proline-Catalyzed Aldol Reactions: (a) Previous
Work and (b) Present Work
a Ee determined by HPLC analysis on a Chiralcel OD-R column using
35% CH3CN in water (0.1% TFA) as an eluent; flow rate ) 1 mL/min.
b Ee of the enantioenriched mother liquor obtained after crystallization of
racemate from CH2Cl2-hexane. c This experiment was carried out in tandem
d
using D,L-proline for amination and L-proline for aldol reaction. D-Proline
is used as a catalyst, and ees are provided for the opposite enantiomers.
The success of this assembly reaction can be attributed to
the higher reactivity of propionaldehyde over acetone in the
proline-catalyzed R-amination reaction. When we compared
the reactivity of these two donors in the proline-catalyzed
R-amination reaction, propionaldehyde exhibited a 100-fold
higher reactivity than acetone when the reactions were
performed under identical conditions in CH3CN. The reaction
conditions for the assembly reaction are very simple: the
reactants are mixed in the presence of catalyst and stirred.
The products are readily purified, and the syn diastereomer
can be enantioenriched by recrystallization from CH2Cl2-
hexane. The two diastereomers can also be separated by
recrystallization from CH2Cl2-hexane. Under D-proline
catalysis, amino alcohols with the opposite stereoconfigu-
rations were obtained.
To further understand the diastereoselectivity and reaction
mechanism, additional experiments were performed. When
we treated racemic aminated propionaldehyde (1) with
acetone in the presence of L-proline, products 2 and 3 were
obtained with yield, diastereoselectivity, and enantioselec-
tivity identical to those found in the reaction involving (R)-
amino aldehyde (1) (Table 1, entry 7, and Scheme 2). Next
we performed a reaction with propionaldehyde and azo-
dicarboxylate for 3 days and determined that the resulting
amino aldehyde was racemic. Thus, proline can act to
racemize the amino aldehyde over time. These two findings
suggest that the reaction proceeds as outlined in Scheme 2.
Amination of propionaldehyde using D,L-proline or L-proline
with extended reaction times (3 days) provides racemic
amino aldehyde (1). The reaction of rac-amino aldehyde (1)
with acetone in the presence of L-proline in CH3CN afforded
through proline catalysis,8 we studied the reaction of acetone,
propionaldehyde, and dibenzyl azodicarboxylate in one pot
with L-proline (20 mol %) in DMSO at room temperature
for 3 days. The functionalized amino alcohol diastereomers
(anti/syn ) 13:87) 2 and 3 were obtained in good yield (72%)
and with an enantioselectivity of >99% for the anti product
(Table 1, entry 1). Given the solvent dependence we have
noted in organocatalytic reactions, we investigated a variety
of solvents for this one-pot reaction. Interestingly the reaction
works well in a range of solvents such as DMSO, DMF,
CH2Cl2, dioxane, acetone, and CH3CN. The use of acetone
as a reactant-solvent also afforded the expected â-amino
alcohols in 68% yield along with aminated acetone in 9%
yield. With the exception of the use of acetone as a solvent,
other solvents suppressed the formation of aminated acetone.
Scaling of the reaction to 20 mmol of reactants afforded
identical results (Table 1, entry 1). Significantly, the use of
DMSO or DMF as solvents enhances the syn selectivity of
the reaction.
(6) (a) Betancort, J. M.; Sakthivel, K.; Thayumanavan, R.; Barbas, C.
F., III. Tetrahedron Lett. 2001, 42, 4441. (b) Betancort, J. M.; Barbas, C.
F., III. Org. Lett. 2001, 3, 3737. (c) Enders, D.; Seki, A. Synlett 2002, 26.
(7) (a) Thayumanavan, R.; Ramachary, D. B.; Sakthivel, K.; Tanaka,
F.; Barbas, C. F., III. Tetrahedron Lett. 2002, 43, 3817. (b) Ramachary, D.
B.; Chowdari, N. S.; Barbas, C. F., III. Tetrahedron Lett. 2002, 43, 6743.
(8) (a) Bogevig, A.; Kumaragurubaran, N.; Juhl, K.; Zhuang, W.;
Jorgensen, K. A. Angew. Chem., Int. Ed. 2002, 41, 1790. (b) Kumaragu-
rubaran, N.; Juhl, K.; Zhuang, W.; Bogevig, A.; Jorgensen, K. A. J. Am.
Chem. Soc. 2002, 124, 6254. (c) List, F. J. Am. Chem. Soc. 2002, 124,
5656.
(9) Chowdari, N. S.; Ramachary, D. B.; Co´rdova, A.; Barbas, C. F., III.
Tetrahedron Lett. 2002, 43, 9591.
1686
Org. Lett., Vol. 5, No. 10, 2003

substituents reacted smoothly in the one-pot assembly
reaction to provide various functionalized amino alcohols
with excellent yields and ees. A â-branched aldehyde
afforded the product with high anti selectivity (Table 2, entry
Scheme 2. Proposed Reaction Pathway for the
Proline-Catalyzed Assembly Reaction
Table 2. Proline-Catalyzed Asymmetric Assembly Reactions
of Acetone and Dibenzyl Azodicarboxylate with a Variety of
Aldehydes
the four possible enantiomers in a ratio of 99:28:229:1
(HPLC data). The amino aldehyde reacts on the exo face of
1-isopropenyl pyrrolidine-2-carboxylic acid, the typical face
selectivity observed in the proline-catalyzed aldol reactions
since this approach facilitates hydrogen bonding between the
carboxylate and the aldehyde acceptor.4 Intermolecular
hydrogen bonding and steric hindrance between enamine and
amino aldehydes as shown in TS 1 and TS 2 then likely
control the facial reactivity of the amino aldehyde. In the
case of (R)-amino aldehyde, intermolecular hydrogen bond-
ing between the carboxylate group and the aldehyde oxygen
is less effective due to steric hindrance between N-substituted
groups or the R-methyl group and the enamine, affording
diastereomers (R,R)-2 and (S,R)-3 with a modest de of 56%.
The (S)-amino aldehyde afforded diastereomers (R,S)-3 and
(S,S)-2 with a very high de of 99%. Here, potential
intermolecular hydrogen bonding between the aldehyde
oxygen and carboxylate group effectively directs the facial
selectivity of the attack of the enamine on the aldehyde. The
enantiofacial selectivity involving (R)-amino aldehydes is
decreased as compared to other known proline-catalyzed
aldol reactions involving simple aldehydes. This is the first
observation that substituent modifications on the acceptor
aldehyde can alter the stereochemistry of proline-catalyzed
aldol reactions. Since racemization of the amination product
is faster than the subsequent aldol reaction, the aldol reaction
occurs between the ketone donor and racemic aminated
propionaldehyde. These reactions can be performed either
in one pot or sequentially since identical results are obtained
in both approaches.
8). The use of ethyl azodicarboxylate as an acceptor afforded
products with yields (80%) and diastereoselectivity (1:1)
analogous to those seen with dibenzyl azodicarboxylate. The
absolute configuration of the amino alcohols prepared here
are assigned on the basis of analogy with our X-ray structure
of the O-acetyl derivative of (R,S)-3 (Figure 1).
Figure 1. X-ray crystal structure of the O-acetyl derivative of (R,S)-
3.
Optically active â-amino alcohol units containing two
stereocenters are often found in natural products and
drugs.10,11 The products of the assembly reactions described
here should be directly applicable as chiral synthons for
Encouraged by these promising results, we next explored
the scope of the assembly reaction using various aldehyde
donors. Aldehydes bearing olefin, aromatic and alicyclic
Org. Lett., Vol. 5, No. 10, 2003
1687

natural product and pharmaceutical synthesis. To demonstrate
this, we have synthesized a known potent renin inhibitor.11
Reaction of the product in entry 3, Table 2, with methyl-
magnesium bromide afforded the corresponding tert-alcohol.
Subsequent deprotection of the hydrazine group using Raney
Ni under hydrogen provided the amino diol renin inhibitor
(Scheme 3).1 Thus, the asymmetric assembly reaction enables
an expedient three-step synthesis of this enzyme inhibitor.
on a multigram scale under operationally simple and safe
conditions. These results provide further evidence that the
assembly of complex products from simple starting materials
is within the realm of organocatalysis involving the simple
naturally occurring amino acid proline. Further studies aimed
at exploring novel amine-catalyzed assembly reactions are
ongoing.
Acknowledgment. This study was supported in part by
the NIH (CA27489) and the Skaggs Institute for Chemical
Biology. We thank Dr. Raj K. Chadha for X-ray structural
analysis.
Scheme 3. Synthesis of a Renin Inhibitor
Supporting Information Available: Experimental pro-
cedures and analytical data for all new compounds. This
material is available free of charge via the Internet at
http://pubs.acs.org.
OL034333N
In conclusion, we have demonstrated for the first time
L-proline-catalyzed enzyme-like assembly of amino alcohols
from three readily available precursors. This simple one-pot
procedure provides direct access to chiral functionalized
amino alcohols. Furthermore, the reactions can be performed
(12) General Experimental Procedure. To a mixture of solvent (3
mL), dibenzyl azodicarboxylate (90%, 330 mg, 1 mmol), L-proline (23 mg,
0.2 mmol), and propionaldehyde (79 µL, 1.1 mmol) was added acetone (2
mL), and the reaction was stirred at room temperature for 72 h. Then, half-
saturated NH4Cl solution and ethyl acetate were added with vigorous stirring;
the layers were separated, and the aqueous phase was extracted thoroughly
with ethyl acetate. The combined organic phases were dried (Na2SO4),
concentrated, and purified by flash column chromatography (silica gel,
mixtures of hexanes/ethyl acetate) to afford the desired amino alcohol as a
white solid as a mixture of diastereomers.
(10) (a) Bergmeier, S. C. Tetrahedron 2000, 56, 2561. (b) Ager, D. J.;
Prakash, I.; Schaad, D. R. Chem. ReV. 1996, 96, 835.
(11) Juraszyk, H.; Schmitges, C. J.; Minck, K. O.; Raddatz, P. (Merck
Patent G.m.b.H., Germany). Eur. Pat. Appl. EP 490259 A2, 1992.
1688
Org. Lett., Vol. 5, No. 10, 2003