Modulation of cytochrome P4501-mediated bioactivation of benzo[a]pyrene by volatile allyl sulfides in human hepatoma cells.

Article abstract of DOI:10.1271/bbb.65.2205

Allyl sulfides such as diallyl sulfide (DAS), diallyl disulfide (DADS), and diallyl trisulfide (DATS), typical flavor components of Allium vegetables, have been shown to inhibit benzo[a]pyrene (B[a]P)-induced carcinogenesis in animal models. As a possible mechanism of this inhibition, the effect of these volatile substances on cytochrome P450 (CYP)1 (CYP1A1, 1A2 and 1B1)-mediated bioactivation of B[a]P was investigated using a human hepatoma cell model (HepG2). DADS and DATS inhibited the B[a]P-induced ethoxyresorufin O-deethylase (EROD) activity, a marker enzyme for CYP1, by 30-90% and 70-95% at 100-1,000 microM concentration, respectively. The cell viability, an indicator of the capacity to inhibit B[a]P bioactivation, was increased by treatments of 100-1,000 microM DADS and 10-100 microM DATS. Immunoblot results indicated that the B[a]P inducible CYP1A2 protein was suppressed by 100-1,000 microM of DADS and 10-100 microM of DATS, but CYP1A1 and 1B1 were not detectable in any microsomes. Analysis of B[a]P metabolites revealed that the level of 7,8-diol formed was significantly reduced in the DADS and DATS treated microsomes as compared to the control. The level of 9,10-diol and 4,5-diol formed was also lowered by the allyl sulfide treatments. These results suggest that the protective mechanism of allyl sulfides on B[a]P-induced carcinogenesis is possibly related with the modulation of CYP1-mediated bioactivation of B[a]P.

Full text of DOI:10.1271/bbb.65.2205

Bioscience, Biotechnology, and Biochemistry
ISSN: 0916-8451 (Print) 1347-6947 (Online) Journal homepage: http://www.tandfonline.com/loi/tbbb20
Modulation of Cytochrome P4501-mediated
Bioactivation of Benzo[a]pyrene by Volatile Allyl
Sulfides in Human Hepatoma Cells
Hyang Sook CHUN, Hyun Jung KIM & Eun Hye CHOI
To cite this article: Hyang Sook CHUN, Hyun Jung KIM & Eun Hye CHOI (2001) Modulation
of Cytochrome P4501-mediated Bioactivation of Benzo[a]pyrene by Volatile Allyl Sulfides in
Human Hepatoma Cells, Bioscience, Biotechnology, and Biochemistry, 65:10, 2205-2212, DOI:
10.1271/bbb.65.2205
To link to this article: http://dx.doi.org/10.1271/bbb.65.2205
Published online: 22 May 2014.
Submit your article to this journal
Article views: 12
View related articles
Citing articles: 16 View citing articles
Full Terms & Conditions of access and use can be found at
http://www.tandfonline.com/action/journalInformation?journalCode=tbbb20
Download by: [Texas A & M International University]
Date: 05 November 2015, At: 15:50