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with 1 mL of diethyl ether and the steroids were separated by
HPLC. Results are expressed as amount of formed andros-
tanedione as percentage of control values.
HPLC procedure
Steroid separation was performed [26] similarly to the method
of Cook et al. [29].The steroids were dissolved in 50 µL metha-
nol and 25 µL was injected into the computer-controlled HPLC
system, which was checked before using labelled reference
controls. Radioactivity was measured using a Berthold LB
506C monitor, using methanol/water (55/45, w/w) for T and
DHT with a flow of 0.4 mL/min and an additive flow of 1.0 mL for
scintillator, baseline separation of T, and DHT was achieved
within 20 min. For the steroids androstenedione and dihydro-
androstenedione methanol/water (50/50, w/w) was used.
Calculation procedure
The amount of DHT formed was calculated (% DHT).The zero
value was subtracted from the control (cv) and inhibition (iv)
values (cvcorr and ivcorr). Inhibition (I) was calculated using the
following equation: % I = (1 – ivcorr/cvcorr)100.
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