Is sulfate lost during the chemical release of oligosaccharides from glycoproteins?

Article abstract of DOI:10.1080/07328309608005423

Model experiments using methyl α-D-galactopyranoside 6-sulfate have implied that the Carlson conditions (1 M sodium borohydride and 0.05 M sodium hydroxide at 45 °C) for the release of O-linked oligosaccharides from glycoproteins do not cause significant loss of sulfate ester groups. However the more vigorous conditions used to release N-linked oligosaccharides are more likely to cause considerable sulfate loss from a galactoside 6-sulfate as a result of 3,6-anhydride formation. Experiments with dextran sulfate suggested that sulfate loss due to oxirane formation was also not significant under the Carlson conditions. These results were supported by experiments with [³⁵S]sulfate-labelled human rectal mucus glycoprotein.

Full text of DOI:10.1080/07328309608005423

This article was downloaded by: [Nanyang Technological University]
On: 24 April 2015, At: 07:41
Publisher: Taylor & Francis
Informa Ltd Registered in England and Wales Registered Number: 1072954 Registered
office: Mortimer House, 37-41 Mortimer Street, London W1T 3JH, UK
Journal of Carbohydrate Chemistry
Publication details, including instructions for authors and
subscription information:
http://www.tandfonline.com/loi/lcar20
Is Sulfate Lost During the Chemical
Release of Oligosaccharides from
Glycoproteins?
Kevin R. King a , J. Michael Williams a , John R. Clamp b & Anthony P.
Corfield b
a Chemistry Department , University of Wales Swansea , Swansea,
SA2 8PP, Wales, UK
b Department of Medicine , University of Bristol , Bristol Royal
Infirmary, Bristol, BS2 8HW, UK
Published online: 22 Aug 2006.
To cite this article: Kevin R. King , J. Michael Williams , John R. Clamp & Anthony P. Corfield (1996)
Is Sulfate Lost During the Chemical Release of Oligosaccharides from Glycoproteins?, Journal of
Carbohydrate Chemistry, 15:1, 41-49, DOI: 10.1080/07328309608005423
To link to this article: http://dx.doi.org/10.1080/07328309608005423
PLEASE SCROLL DOWN FOR ARTICLE
Taylor & Francis makes every effort to ensure the accuracy of all the information (the
“Content”) contained in the publications on our platform. However, Taylor & Francis,
our agents, and our licensors make no representations or warranties whatsoever as to
the accuracy, completeness, or suitability for any purpose of the Content. Any opinions
and views expressed in this publication are the opinions and views of the authors,
and are not the views of or endorsed by Taylor & Francis. The accuracy of the Content
should not be relied upon and should be independently verified with primary sources
of information. Taylor and Francis shall not be liable for any losses, actions, claims,
proceedings, demands, costs, expenses, damages, and other liabilities whatsoever or
howsoever caused arising directly or indirectly in connection with, in relation to or arising
out of the use of the Content.
This article may be used for research, teaching, and private study purposes. Any
substantial or systematic reproduction, redistribution, reselling, loan, sub-licensing,
systematic supply, or distribution in any form to anyone is expressly forbidden. Terms &
Conditions of access and use can be found at http://www.tandfonline.com/page/terms-
and-conditions