Journal of Carbohydrate Chemistry p. 41 - 49 (1996)
Update date:2022-08-05
Topics:
King, Kevin R.
Williams, J. Michael
Clamp, John R.
Corfield, Anthony P.
Model experiments using methyl α-D-galactopyranoside 6-sulfate have implied that the Carlson conditions (1 M sodium borohydride and 0.05 M sodium hydroxide at 45 °C) for the release of O-linked oligosaccharides from glycoproteins do not cause significant loss of sulfate ester groups. However the more vigorous conditions used to release N-linked oligosaccharides are more likely to cause considerable sulfate loss from a galactoside 6-sulfate as a result of 3,6-anhydride formation. Experiments with dextran sulfate suggested that sulfate loss due to oxirane formation was also not significant under the Carlson conditions. These results were supported by experiments with [35S]sulfate-labelled human rectal mucus glycoprotein.
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