Accumulation of ergosta 8,14 dien 3β ol by Saccharomyces cerevisiae cultured with an azasterol antimycotic agent

Article abstract of DOI:10.1007/BF02533762

15-Aza-24-methylene-D-homocholesta-8,14-dien-3β-ol, an antimycotic agent, at a concentration of 75 ng/ml inhibited ergosterol biosynthesis in S. cerevisiae strain 3701B resulting in the accumulation of an unusual sterol. Experimental data presented indicate that this sterol is ergosta-8,14-dien-3β-ol. The accumulation of the compound is supportive of current models of biosynthetic pathways for sterols in yeast and is consistent with inhibition by the azasterol of the Δ¹⁴ sterol reductase.