A Global Scale Scenario for Prebiotic Chemistry: Silica-Based Self-Assembled Mineral Structures and Formamide

Article abstract of DOI:10.1021/acs.biochem.6b00255

The pathway from simple abiotically made organic compounds to the molecular bricks of life, as we know it, is unknown. The most efficient geological abiotic route to organic compounds results from the aqueous dissolution of olivine, a reaction known as serpentinization (Sleep, N.H., et al. (2004) Proc. Natl. Acad. Sci. USA 101, 12818-12822). In addition to molecular hydrogen and a reducing environment, serpentinization reactions lead to high-pH alkaline brines that can become easily enriched in silica. Under these chemical conditions, the formation of self-assembled nanocrystalline mineral composites, namely silica/carbonate biomorphs and metal silicate hydrate (MSH) tubular membranes (silica gardens), is unavoidable (Kellermeier, M., et al. In Methods in Enzymology, Research Methods in Biomineralization Science (De Yoreo, J., Ed.) Vol. 532, pp 225-256, Academic Press, Burlington, MA). The osmotically driven membranous structures have remarkable catalytic properties that could be operating in the reducing organic-rich chemical pot in which they form. Among one-carbon compounds, formamide (NH₂CHO) has been shown to trigger the formation of complex prebiotic molecules under mineral-driven catalytic conditions (Saladino, R., et al. (2001) Biorganic & Medicinal Chemistry, 9, 1249-1253), proton irradiation (Saladino, R., et al. (2015) Proc. Natl. Acad. Sci. USA, 112, 2746-2755), and laser-induced dielectric breakdown (Ferus, M., et al. (2015) Proc Natl Acad Sci USA, 112, 657-662). Here, we show that MSH membranes are catalysts for the condensation of NH₂CHO, yielding prebiotically relevant compounds, including carboxylic acids, amino acids, and nucleobases. Membranes formed by the reaction of alkaline (pH 12) sodium silicate solutions with MgSO₄ and Fe₂(SO₄)₃·9H₂O show the highest efficiency, while reactions with CuCl₂·2H₂O, ZnCl₂, FeCl₂·4H₂O, and MnCl₂·4H₂O showed lower reactivities. The collections of compounds forming inside and outside the tubular membrane are clearly specific, demonstrating that the mineral self-assembled membranes at the same time create space compartmentalization and selective catalysis of the synthesis of relevant compounds. Rather than requiring odd local conditions, the prebiotic organic chemistry scenario for the origin of life appears to be common at a universal scale and, most probably, earlier than ever thought for our planet.

Full text of DOI:10.1021/acs.biochem.6b00255

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Article
pubs.acs.org/biochemistry
A Global Scale Scenario for Prebiotic Chemistry: Silica-Based Self-
Assembled Mineral Structures and Formamide
Raffaele Saladino,*,† Giorgia Botta,† Bruno Mattia Bizzarri,† Ernesto Di Mauro,‡
and Juan Manuel Garcia Ruiz*,§
†Dipartimento di Scienze Ecologiche e Biologiche, Universita
̀
della Tuscia, Via San Camillo De Lellis, 01100 Viterbo, Italy
‡Istituto Pasteur-Fondazione Cenci Bolognetti c/o Dipartimento di Biologia e Biotecnologie “Charles Darwin”, University “Sapienza”,
Piazzale Aldo Moro 5, Rome 00185, Italy
§Laboratorio de Estudios Crystalografi
́
cos, Instituto Andauz de Ciencias de la Tierra, CSIC-Universidad de Granada, Avenida de las
Palmeras 4, E-18100 Armilla, Granada, Spain
S
* Supporting Information
ABSTRACT: The pathway from simple abiotically made organic compounds to the molecular
bricks of life, as we know it, is unknown. The most efficient geological abiotic route to organic
compounds results from the aqueous dissolution of olivine, a reaction known as serpentinization
(Sleep, N.H., et al. (2004) Proc. Natl. Acad. Sci. USA 101, 12818−12822). In addition to molecular
hydrogen and a reducing environment, serpentinization reactions lead to high-pH alkaline brines
that can become easily enriched in silica. Under these chemical conditions, the formation of self-
assembled nanocrystalline mineral composites, namely silica/carbonate biomorphs and metal
silicate hydrate (MSH) tubular membranes (silica gardens), is unavoidable (Kellermeier, M., et al.
In Methods in Enzymology, Research Methods in Biomineralization Science (De Yoreo, J., Ed.) Vol.
532, pp 225−256, Academic Press, Burlington, MA). The osmotically driven membranous
structures have remarkable catalytic properties that could be operating in the reducing organic-rich
chemical pot in which they form. Among one-carbon compounds, formamide (NH2CHO) has
been shown to trigger the formation of complex prebiotic molecules under mineral-driven catalytic
conditions (Saladino, R., et al. (2001) Biorganic & Medicinal Chemistry, 9, 1249−1253), proton
irradiation (Saladino, R., et al. (2015) Proc. Natl. Acad. Sci. USA, 112, 2746−2755), and laser-induced dielectric breakdown
(Ferus, M., et al. (2015) Proc Natl Acad Sci USA, 112, 657−662). Here, we show that MSH membranes are catalysts for the
condensation of NH2CHO, yielding prebiotically relevant compounds, including carboxylic acids, amino acids, and nucleobases.
Membranes formed by the reaction of alkaline (pH 12) sodium silicate solutions with MgSO4 and Fe2(SO4)3·9H2O show the
highest efficiency, while reactions with CuCl2·2H2O, ZnCl2, FeCl2·4H2O, and MnCl2·4H2O showed lower reactivities. The
collections of compounds forming inside and outside the tubular membrane are clearly specific, demonstrating that the mineral
self-assembled membranes at the same time create space compartmentalization and selective catalysis of the synthesis of relevant
compounds. Rather than requiring odd local conditions, the prebiotic organic chemistry scenario for the origin of life appears to
be common at a universal scale and, most probably, earlier than ever thought for our planet.
he geological period lasting from the accretion of the
Tplanet to 3.8 giga-anni (Ga) is named the Hadean. This
name refers to Hades, lord of the Underworld, because that
period was thought to be under such infernal conditions as high
volcanic activity, high temperature, and intense meteoritic
impacts such that even the preservation of rocks was impeded.
However, recent isotopic analyses of the zircon crystals found
in the Australian localities of Mt. Narryer6 and Jack Hills7 have
changed that view. These geochemical analyses demonstrated
that water might have existed in the liquid state much earlier
than currently believed. If the age of the Earth is calculated to
be 4.55 Ga,8 some of these zircon crystals show that water
condensed on the rocks of the surface of the planet as early 150
million years later.9,10 Organic carbon chemistry was thus
already plausible 4.4 billion years ago. On such a young planet,
water should have condensed on ultramafic rocks, which
consisted of a scarce variety of minerals, mostly olivine and
pyroxenes of variable cationic composition. This geochemical
scenario intrinsically triggered serpentinization on a global,
planet scale.11 Serpentinization reactions entail a reductive
environment rich in H2 and CH4, the latter formed upon
reaction with CO2 outgassing from the inner mantle.12,13 The
Fischer−Tropsch process should have driven chemical
reactions, leading to the formation of further complex organic
molecules.14,15 The above-described geological scenario of
weathered olivine and pyroxene minerals covering the surface
of the planet led to water pools of high pH (about 12) and,
upon interactions with plagioclase, quartz, or other silica-rich
rocks and minerals, high silica concentration. Under these
Received: March 23, 2016
Revised: April 26, 2016
Published: April 26, 2016
© 2016 American Chemical Society
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Figure 1. (A) Silica/barium carbonate biomorph. (B) Calcium silicate hydrate tubular membranes formed upon the interaction of a highly alkaline
fluid with a granitic rock.20 (C) Interaction of a pellet of a soluble salt with a highly alkaline solution of silica produces the formation around the
pellet of a thin membrane of metal silicate and hydroxides. The porous membrane works as a diaphragm, allowing the interchange of ions between
the outer strongly alkaline silicate solution and the inner concentrated acidic solutions.18 Osmosis causes bursting of the membrane and injection of
the inner solution into the outer one, which accounts for the formation of the tubular forms. (D) Iron silicate hydrate tubes formed in the presence
of (from left to right) 0%, 2%, 5%, and 10% (w/w) formamide. (E) Cross section of the iron silicate hydrate membrane showing the textural and
compositional gradient. (F) Iron silicate hydrate hollow microspheres formed by an acidic FeCl3 solution being sprayed in the alkaline sodium
silicate solution, and a sketch of these structures expected to form in the early, lifeless planet is shown.
alkaline chemical conditions, silica is known to react with
metals to form fascinating self-assembled mineral structures. At
these pH values, barium, strontium, and/or calcium carbonate
form silica biomorphs which are made by self-assembled
carbonate nanocrystals, building textures of high complexity
and showing morphologies with continuous curvature reminis-
cent of primitive living organisms and Precambrian microfossils
(Figure 1A).16,17
experimentally demonstrated.18 Finally, the metal silicate
hydrate character of the membrane creates not only a
compartmentalized volume but also a shield against ultraviolet
radiation.26
Among the plausible one-carbon atom chemical precursors
that can be produced by purely geochemical pathways (HCN,
HCOH, HCOOH, NH2CHO, and HCOONH4), we selected
formamide for our experiments. Formamide (NH2CHO) has
been shown to be highly active under mineral catalysis
conditions.3,4,27 NH2CHO is largely diffused in the universe
and has been detected in parsec-wide interstellar clouds.28,29
The fact that NH2CHO is liquid between 4 and 210 °C makes
it particularly suited to concentration phenomena such as
thermoconvection on rock.30 Space and terrestrial syntheses of
NH2CHO under a large variety of conditions have been
previously described and explained.5,26,30
In addition, the reaction of alkaline silica solution with metals
such as zinc, iron, cobalt, magnesium, nickel, copper, etc.,
provokes the formation of a metal silicate hydrate (MSH)
membrane and the spontaneous separation of two solutions
with strong chemical differences.18 A morphogenetic mecha-
nism based on a combination of osmosis, buoyancy, and
chemical reaction results in hollow tubular architectures known
as chemical gardens. Their osmotically driven formation and
shape look so biological that they have long been linked to the
origin of life,19 and more recently, they helped to develop a
theory on the origin of life in white smokers.20,21 These MSH
structures have been made in granitic rocks weathered by highly
alkaline fluids22 (Figure 1B), but their plausible role in prebiotic
chemistry has never been tested. The catalytic role of minerals
in the prebiotic synthesis of biologically relevant compounds
from simple chemical precursors is well-known.23−25 The
novelty and main interest that these self-assembled mineral
structures bring to the search for mineral routes to life is
twofold. First, the metal silicate hydrate phases have been
shown to share the properties of a diaphragm and a membrane,
displaying a textural and compositional gradient that allows
selective catalytic properties of their inner and outer parts.
Second, the membrane separates an enclosed volume of
concentrated acidic metal salt solution from the surrounding
strongly alkaline silica solution. The existence of an electro-
chemical potential difference of 20−120 mV that lasts for
several hours between the two compartments has been
MATERIALS AND METHODS
■
Formamide (Fluka, >99%) was used without further
purification. Fresh commercial water glass (Sigma-Aldrich,
reagent grade, containing about 13.8 wt % Na and 12.5 wt %
Si) was used as the silica source and was further diluted 1/4 (v/
v) with Millipore water. We obtained silica gardens by dipping
small pellets of a metal soluble salt into the (diluted) sodium
silicate solution containing 2%, 5%, or 10% (v/v) NH2CHO
(see Figure 1 and Supplementary Video 1). Different metal
soluble salts were used, namely, ZnCl2, FeCl2·4H2O, CuCl2·
2H2O, Fe2(SO4)3·9H2O, and MgSO4.
To model the chemical environment on the outer side of the
tubular structures, NH2CHO (200 μL) was mixed with the
sodium silicate solution (2.0 mL) in the presence of preformed
MSH [ZnCl2, FeCl2·4H2O, CuCl2·2H2O, Fe2(SO4)3·9H2O,
and MgSO4] (2.0% w/w) at 80 °C for 24 h. In two selected
cases [FeCl2 and Fe2(SO4)3·9H2O], NH2CHO (200 μL) was
mixed with the sodium silicate solution (2.0 mL) in the
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presence of selected growing MSH (starting from 2.0% w/w of
the corresponding salt’s pellet) at 80 °C for 24 h. For the inner
environment, NH2CHO (200 μL) was mixed with distilled
water (2.0 mL) in the presence of selected MSH (2.0% w/w) at
80 °C for 24 h. The reaction of NH2CHO (10% v/v) with the
sodium silicate solution (pH 12) without MSH membranes was
also analyzed under similar experimental conditions. The
products were analyzed by gas chromatography associated
with mass spectrometry (GC-MS) after treatment with N,N-
bis-trimethylsilyl trifluoroacetamide in pyridine (620 μL) at 60
°C for 4 h in the presence of betulinol (CAS Registry Number
473-98-3) as the internal standard (0.2 mg). Mass spectrometry
was performed by the following program: injection temperature
280 °C, detector temperature 280 °C, gradient 100 °C for 2
min, and 10 °C/min for 60 min. To identify the structure of the
products, two strategies were followed. First, the spectra were
compared with commercially available electron mass spectrum
libraries such as NIST (Fison, Manchester, U.K.). Second, GC-
MS analysis was repeated with standard compounds. All
products have been recognized with a similarity index (SI)
greater than 98% compared to that of the reference standards.
The analysis was limited to products of ≥1 ng/mL, and the
yield was calculated as micrograms of product per starting
formamide. For further experimental details, see the Supporting
Information.
Supplementary Video 1, metal silicate hydrate tubular
structures can be formed readily in the presence of up to
10% NH2CHO by small pellets of different metal soluble salts
being dispensed inside a sodium silicate solution (SSS)
containing 2%, 5%, or 10% (v/v) NH2CHO (see Methods).
Then, we designed two different kinds of experiments to model
the chemical environment in the outer and inner parts of the
tubular structure according to previous work. In the first
experiment, we dipped selected and preformed MSH tubules
[ZnCl2, FeCl2·4H2O, CuCl2·2H2O, Fe2(SO4)3·9H2O, or
MgSO4] in an alkaline (pH 12) solution of sodium silicate
containing 10% (v/v) NH2CHO. In two selected cases [FeCl2
and Fe2(SO4)3·9H2O], the first experiment was repeated in the
presence of the membrane in growth by the addition of the
pellets of soluble salts directly inside the sodium silicate
solution. In the second experiment, we modeled the chemistry
of the inner part of the tubular structures by dipping the
selected MSH membrane in a water solution and 10% (v/v)
NH2CHO. As a control experiment, we also analyzed the
output of an alkaline solution (pH 12) of sodium silicate with
NH2CHO (10% v/v) in the absence of MSH membranes. All
of the experiments ran for 24 h at the optimized temperature of
80 °C. The experiments were reproduced three times.
The results of the experiments are shown in Tables 1−3 and
Figure 2 (see also the Supporting Information). As a general
trend, the control reaction at 80 °C afforded a panel of
compounds larger than that obtained at 25 and 120 °C.
Guanidine (2), urea (3), pyruvic acid (4), lactic acid (5), and
glycolic acid (6) were observed in small amounts (Table 1).
The temperature of 80 °C was selected for the next reactions.
The experiments that used preformed MSH membranes
afforded a larger variety of products and did so in higher
yields (Tables 2 and 3, respectively). In particular, the
experiment modeling the catalytic effect of the outer side of
the membrane afforded 2, 3, 4, 5, 6, oxalic acid (7), succinic
acid (8), malic acid (9), N-formylglycine (10), and diamino
malonitrile (DAMN) (11) (Table 2 and Figure 2).
RESULTS AND DISCUSSION
We first demonstrated the formation of silica gardens in the
presence of NH2CHO. As shown in Figure 1D and
■
Table 1. Products Obtained (mg) after the Reaction of
NH2CHO with Sodium Silicate Solution at Different
a
Temperatures
product
25 °C
traces
1.0 × 10−3
1.1 × 10−3
0.7 × 10−3
traces
80 °C
traces
2.2 × 10−3
1.9 × 10−3
1.0 × 10−3
traces
120 °C
traces
0.5 × 10−3
0.3 × 10−3
traces
guanidine (2)
urea (3)
pyruvic acid (4)
lactic acid (5)
glycolic acid (6)
Similar results were obtained in the presence of the growing
membrane (Table 2, data in parentheses), suggesting that the
“active” silicate membranes do not play a key role when
compared to that of preformed membranes.
a
NH2CHO (200 μL) was mixed with the sodium silicate solution (2.0
mL) at the reported temperatures for 24 h. The data are the mean
Interestingly, the chemical environment modeling the inner
side of the tubular structures afforded an even larger panel of
products. In addition to compounds 2−11, the inner
values of three experiments with standard deviations of less than 0.1%.
a
Table 2. Products Obtained after the Reaction of NH2CHO and SSS in the Presence of Selected MSH
b,c
product
ZnCl2
FeCl2
CuCl2
Fe2(SO4)3
MgSO4
traces
d
d
guanidine (2)
0.90 (0.40)
0.80 (0.32)
0.83 (0.24)
0.63 (0.92)
0.01 (0.12)
0.18 (nd)
traces
0.90
0.80 (0.90)
c
e
urea (3)
2.0 × 10−3
1.9 × 10−3
0.15
0.015 (nd)
0.01
pyruvic acid (4)
lactic acid (5)
traces
traces
0.15 (0.05)
0.16 (0.11)
traces
glycolic acid (6)
oxalic acid (7)
succinic acid (8)
malic acid (9)
0.11
2.8 × 10−3
0.11
0.38 (0.25)
0.096 (0.01)
0.02 (0.06)
9.0 × 10−3 (nd)
0.13 (0.64)
0.12
0.16
0.071
0.005
2.3 × 10−3
0.09
N-formylglycine (10)
DAMN (11)
6.0 × 10−3
traces (nd)
0.46 (nd)
a
b
NH2CHO (200 μL) was mixed with SSS (2.0 mL) in the presence of preformed MSH (2.0% w/w) at 80 °C for 24 h. The data are the mean
c
values of three experiments with standard deviations of less than 0.1%. The amount of product is defined as milligrams of compound compared to
that of the initial reaction mixture. NH2CHO (200 μL) was mixed with SSS (2.0 mL) in the presence of selected growing MSH (starting from 2.0%
w/w of the corresponding salt’s pellet) at 80 °C for 24 h. nd = not determined.
d
e
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a
Table 3. Products Obtained after the Reaction of NH2CHO and Distilled Water in the Presence of Specific MSH
b
product
ZnCl2
FeCl2
3.4 × 10−3
CuCl2
1.6
MnCl2
Fe2(SO4)3
0.18
MgSO4
CuN2O6
0.67
c
guanidine 2
5.2 × 10−3
traces
4.1 × 10−3
0.1
5.0 × 10−3
0.12
traces
0.7
urea 3
1.7
1.8 × 10−3
pyruvic acid (4)
lactic acid (5)
oxalic acid (7)
succinic acid (8)
malic acid (9)
N-formylglycine (10)
DAMN (11)
0.01
0.73
0.07
traces
0.21
0.03
1.9
0.28
0.18
0.05
traces
0.17
0.03
1.8
traces
2.5
0.10
0.85
traces
0.02
0.57
0.27
traces
0.53
0.23
glycine (12)
0.03
0.23
0.76
traces
0.59
5.6
alanine (13)
traces
traces
parabanic acid (14)
4(3H)-Pyr (15)
2,4-DAP (16)
6(OH)-2,4-DAP (17)
2,4-DAP-5COOH (18)
cytosine (19)
0.9
0.3
traces
0.18
traces
0.17
0.05
traces
traces
0.14
1.2
0.3
0.02
0.06
0.3
traces
0.13
5.0
traces
0.18
0.11
0.22
0.01
traces
0.15
0.11
0.23
0.01
traces
traces
isocytosine (20)
uracil (21)
1.4
traces
3.8
0.03
0.85
traces
adenine (22)
0.01
a
b
NH2CHO (200 μL) was mixed with water (2.0 mL) in the presence of selected MSH (2.0% w/w) at 80 °C for 24 h. The data are the mean values
c
of three experiments with standard deviations of less than 0.1%. The amount of product is defined as milligrams of compound compared to that of
the initial reaction mixture.
Figure 2. Schematic representation of products obtained with different MSH from NH2CHO outside (left) and inside (right) of the membranous
structure. Color codes: compounds synthesized at both sides of the membrane (green); compounds synthesized only inside the membrane (blue).
environment of the membranes also catalyzed glycine (12),
alanine (13), parabanic acid (14), 4(3H)pyrimidinone [4(3H)-
Pyr] (15), 2,4-diamino pyrimidine (2,4-DAP) (16), 6-hydroxy-
2,4-diamino pyrimidine [6(OH)-2,4-DAP] (17), 2,4-diamino
pyrimidine-5-carboxylic acid (2,4-DAP-5COOH) (18), cyto-
sine (19), isocytosine (20), uracil (21), and adenine (22)
(Table 3 and Figure 2). MgSO4, Fe2(SO4)3·9H2O, CuN2O6·
3H2O, and CuCl2·2H2O were the most active MSH in the
synthesis of nucleobases 19, 21, and 22. Amino acids 12 and 13
were also produced in acceptable amounts
It is interesting to note that the salts of the two metals that
form the olivine solid solution, MgSO4 and Fe2(SO4)3·9H2O,
are the most efficient salts, while CuCl2·2H2O, ZnCl2, FeCl2·
4H2O, and MnCl2·4H2O showed a low reactivity. Carboxylic
acids 4−9 were obtained in larger amounts in the outer
environment with the exception of only MgSO4 and Fe2(SO4)3·
9H2O. Therefore, this process is selective in terms of mineral
properties because different metal silicate hydrated membranes
afford different panels of products. Even more interesting is the
fact that the panels of compounds formed inside and outside
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the tubular membrane are specific, as shown in Figure 2. Thus,
nucleobases (19, 21, and 22), nucleobase bioisosteres (16 and
20), and nucleobase analogues (15, 17, and 18) are produced
only inside the membrane (Figure 2). Amino acids 12 and 13
are also synthesized only inside the membrane.
AUTHOR INFORMATION
Corresponding Authors
*E-mail: saladino@unitus.it.
*E-mail: juanma.garciaruiz@gmail.com.
■
Author Contributions
Carboxylic acids 4−9 were obtained in the inner and outer
environments. The prebiotic roles of DAMN31 and the
nucleobase bioisosteres and analogues have been reviewed
previously.32 The ability of formamide to trigger the synthesis
of compounds representative of the major classes of prebiotic
precursors in the presence of a number of minerals (including
boron-, iron-, sulfur-, zircon-, titanium-, and phosphorus-based
minerals, metal oxides of various types, and meteorites) has
been previously shown to be particularly efficient under proton
irradiation and the simulated impact of an extraterrestrial body.
Our results provide the first example of a catalytic process
endowed with (a) selective catalysis of the synthesis of biogenic
relevant compounds by a textured membrane, (b) intrinsic
compartmentalization ability, and (c) a shielding environment
against ultraviolet radiation. Interestingly, in the reported
experiments, the nucleic acid precursors were located on the
inner side of the membranes. However, we have not found
significant differences in either the number or the yield of
biochemically relevant compounds when comparing the
reactivities of active versus passive metal silicate hydrate
membranes. This means that the electron voltage reported in
previous silica garden18 experiments does not play a differential
role in the catalysis of prebiotic compounds. The geological
niche proposed here for the transition from inorganic to
organic geochemistry, a silica-rich, alkaline, aqueous solution in
contact with metal-bearing minerals in the presence of
NH2CHO, was highly plausible during the Hadean and
Archean times. This niche was settled most likely as early as
4.4 Ga, i.e., almost one billion years earlier than the oldest
putative remnants of life on our planet. Therefore, the existence
of biological compounds such as carboxylic acids, amino acids,
and nucleobases, or their carbon-like derivatives in Hadean
zircon crystals or in Archean rocks, is rather plausible. It is
worth noting that in these organic geoniches, silica biomorphs
that mimic primitive organisms readily form in the presence of
alkaline earth metals.16 This geological niche is not exclusive to
our planet. It should also exist, or have existed, on Earth-like
planets, meteorite parent bodies, and comets, as well as in the
interstellar dust made of olivine that are or were in contact with
enriched regions of NH2CHO in the universe. These results
suggest that the conditions required for the synthesis of the
molecular bricks from which life self-assembles, rather than
being local and bizarre, seem to be universal and geologically
conventional.
R.S., G.B., and B.M.B. performed the condensation experiments
with formamide, and J.M.G.R. performed the silica garden
experiments. E.D.M. and J.M.G.R. conceived the work.
J.M.G.R., R.S., and E.D.M. analyzed the results and wrote the
manuscript.
Funding
The research behind this work received funding from the
European Research Council under the European Union’s
Seventh Framework Programme (FP7/2007−2013)/ERC
Grant Agreement 340863.
Notes
The authors declare no competing financial interest.
ACKNOWLEDGMENTS
■
The Italian Space Agency (ASI) project “Esobiologia e
Ambienti estremi: dalla chimica delle Molecola alla Biologia
degli estremofili” and COST Action TD 1308 are acknowl-
edged. J.M.G.R. acknowledges the help of Francisca Espinosa
with the experiments. The CGA of the University of Tuscia is
acknowledged.
ABBREVIATIONS
■
MSH, metal silicate hydrate; SSS, sodium silicate solution;
DAMN, diamino malonitrile; [4(3H)-Pyr], 4(3H)-
pyrimidinone; (2,4-DAP), 2,4-diamino pyrimidine; [6(OH)-
2,4-DAP], 6-hydroxy-2,4-diamino pyrimidine; (2,4-DAP-
5COOH), 2,4-diamino pyrimidine-5-carboxylic acid
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