Synthesis of New 1-Substituted-3-(3-(2-chlorophenyl)-4-oxo-3,4-dihydrobenzopyrimidin-2-ylamino)isothioureas
59
one-way analysis of variance (ANOVA). In all cases, posthoc
comparisons of the means of individual groups were per-
formed using Tukey’s test. For statistical analysis, the
GraphPad Prism version 5.0 was used.
bonate (100 mg, 7.2 mmol) was added to the above mixture
and refluxed for 25 h. Using ice the reaction mixture was
cooled, and the solid separated was filtered. The obtained
product 3 was purified by dissolving 10% alcoholic sodium
hydroxide solution (10 mL) and re-precipitated by treating
with dilute hydrochloric acid (5.5 mL) to get pH 3.0. The
solid obtained was filtered, washed with water, dried and
recrystallized from ethanol.
CONFLICT OF INTEREST
The authors declare that they have no conflict of interest.
2-Methylthio-3-(2-chlorophenyl) benzopyrimidin-4-
one (4). 3-(2-chlorophenyl)-2-thioxo-2,3-dihydro benzopyri-
midin-4-one (3) (0.01 mol, 2.89 g) was dissolved in alco-
holic sodium hydroxide solution (25 mL). To this dimethyl
sulphate (0.01 mol, 1.26 g) was added drop wise with stirring
and stirring was continued for 1 h after complete addition of
dimethyl sulphate. The reaction mixture was then poured
into ice-cold water. The solid obtained was filtered, washed
with water, dried and recrystallized from ethanol to afford
compound 4 (3.02 g) [19 – 20].
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