ELECTROCHEMICAL DETECTION OF REDUCING CARBOHYDRATES PRODUCED BY THE TRANSFERASE ACTION OF YEAST DEBRANCHING ENZYME ON MALTOSACCHARIDES

Article abstract of DOI:10.1016/0008-6215(88)80136-8

A sensitive method for the detection of maltosaccharides up to maltoheptaose is based on an electrochemical detector using bis(1,10-phenanthroline)-copper(II) in the post column reaction after h.p.l.c. on an amino-bonded column.This method has been used for the analysis of the maltosyl and maltotriosyl transferase action of the yeast debranching enzyme with maltosaccharides as the substrates.The smallest donor substrate for maltosyl transfer was maltotetraose, and maltopentaose, maltohexaose, and maltoheptaose were donor substrates for both maltosyl and maltotriosyl transfers.Maltosyl residues were transferred in preference to maltotriosyl residues from maltopentaose, but maltotriosyl residues were transferred prefentially from maltohexaose and maltoheptaose.Maltotriose is an acceptor but not a donor of maltosyl and maltotriosyl transfers.