13C NMR SPECTRA AND BIOLOGICAL ACTIVITY
1659
phytopathogenic fungi in the presence of a compound
to be tested. All compounds were tested as a concen-
tration of 0.003%. To ensure uniform distribution over
the substrate, compounds were preliminarily dissolved
in acetone (1:150) or thoroughly powdered. Potato
glucose agar was used as nutrient medium. Com-
pounds to be tested were added to the substrate before
its congelation (at 45 50 C), and the medium was
transferred onto Petri dishes in a volume of 15 cm3.
Fusarium culmorum and Helminthosporium sativum
mycelia were sown into each Petri dish at three sites
simultaneously. In a blank sample, were sown into
the same nutrient medium containing no fungicide.
Samples were incubated for 72 h at 25 26 C, and
average diameters of the fungi colonies were mea-
sured. Carbendazim (I) (a 30% suspension) was tested
under the same conditions for comparison. The
fungicide activity was calculated by the known
formula [29].
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