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8. Gigant, B.; Wang, C.; Ravelli, R. B. G.; Roussi, F.;
References and notes
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12. Unpublished results.
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14. Typical procedure: into a 0.1 N NaHCO3 solution and a
solution of 302 mg of 2 in 12 ml H2O was bubbled Ar for
10 min. With continuous Ar bubbling, the bicarbonate
solution was added to the solution of 2 so as to adjust the
pH to ca. 7 (maintain Ar bubbling in the reaction vessel
throughout the procedure). A solution of 258 mg of the 7
in 13 ml THF was then added to the solution of 2. The
reaction mixture was allowed to stir for 15 min. At this
point HPLC indicated that reaction was complete. The
THF was removed under reduced pressure. The resulting
mixture was centrifuged. The aqueous layer was syringe
filtered and loaded onto the preparative HPLC. HPLC
purification using 10.0 mM sodium phosphate buffer (pH
7) and acetonitrile, followed by a second HPLC run with
water and acetonitrile to remove the salt, resulted in the
recovery of 350 mg of product.
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