Structure-activity relationship of oligomeric flavan-3-ols: Importance of the upper-unit B-ring hydroxyl groups in the dimeric structure for strong activities

Article abstract of DOI:10.3390/molecules201018870

Proanthocyanidins, which are composed of oligomeric flavan-3-ol units, are contained in various foodstuffs (e.g., fruits, vegetables, and drinks) and are strongly biologically active compounds. We investigated which element of the proanthocyanidin structu

Full text of DOI:10.3390/molecules201018870

Molecules 2015, 20, 18870-18885; doi:10.3390/molecules201018870
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molecules
ISSN 1420-3049
www.mdpi.com/journal/molecules
Article
Structure–Activity Relationship of Oligomeric Flavan-3-ols:
Importance of the Upper-Unit B-ring Hydroxyl Groups in the
Dimeric Structure for Strong Activities
Yoshitomo Hamada 1, Syota Takano 2, Yoshihiro Ayano 1, Masahiro Tokunaga 2,
Takahiro Koashi 1, Syuhei Okamoto 1, Syoma Doi 1, Masahiko Ishida 2,
Takashi Kawasaki 3, Masahiro Hamada 4, Noriyuki Nakajima 4,* and Akiko Saito 1,2,
*
1
Graduate School of Engineering, Osaka Electro-Communication University (OECU),
18-8 Hatsu-cho, Neyagawa-shi, Osaka 572-8530, Japan; E-Mails: me14a007@oecu.jp (Y.H.);
me14a001@oecu.jp (Y.A.); me14a005@oecu.jp (T.K.); me12a003@oecu.jp (S.O.);
me12a008@oecu.jp (S.D.)
2
Faculty of Engineering, Osaka Electro-Communication University (OECU),
18-8 Hatsu-cho, Neyagawa-shi, Osaka 572-8530, Japan; E-Mails: eg10a032@osakac.info (S.T.);
eu11a066@oecu.jp (M.T.); eg10a006@osakac.info (M.I.)
3
4
Department of Pharmaceutical Sciences, Ritsumeikan University, 1-1-1 Nojihigashi, Kusatsu,
Shiga 525-8577, Japan; E-Mail: kawa0227@fc.ritsumei.ac.jp
Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University,
5180, Kurokawa, Imizu, Toyama 939-0398, Japan; E-Mail: hamada@pu-toyama.ac.jp
* Authors to whom correspondence should be addressed; E-Mails: nori@pu-toyama.ac.jp (N.N.);
a-saito@isc.osakac.ac.jp (A.S.); Tel.: +81-766-56-7500 (N.N.); +81-72-824-1131 (A.S.);
Fax: +81-766-56-2498 (N.N.); +81-72-824-0014 (A.S.).
Academic Editor: Thomas J. Schmidt
Received: 30 June 2015 / Accepted: 7 October 2015 / Published: 16 October 2015
Abstract: Proanthocyanidins, which are composed of oligomeric flavan-3-ol units, are
contained in various foodstuffs (e.g., fruits, vegetables, and drinks) and are strongly biologically
active compounds. We investigated which element of the proanthocyanidin structure is primarily
responsible for this functionality. In this study, we elucidate the importance of the upper-unit of
4–8 condensed dimeric flavan-3-ols for antimicrobial activity against Saccharomyces cerevisiae
(S. cerevisiae) and cervical epithelioid carcinoma cell line HeLa S3 proliferation inhibitory
activity. To clarify the important constituent unit of proanthocyanidin, we synthesized four
dimeric compounds, (−)-epigallocatechin-[4,8]-(+)-catechin, (−)-epigallocatechin-[4,8]-(−)-

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epigallocatechin, (−)-epigallocatechin-[4,8]-(−)-epigallocatechin-3-O-gallate, and (+)-catechin-
[4,8]-(−)-epigallocatechin and performed structure–activity relationship (SAR) studies.
In addition to antimicrobial activity against S. cerevisiae and proliferation inhibitory activity
on HeLa S3 cells, the correlation of 2,2-diphenyl-l-picrylhydrazyl radical scavenging activity
with the number of phenolic hydroxyl groups was low. On the basis of the results of our SAR
studies, we concluded that B-ring hydroxyl groups of the upper-unit of the dimer are crucially
important for strong and effective activity.
Keywords: condensed tannins; oligomeric flavonoid; synthesis; DPPH radical scavenging
activity; antimicrobial activity; cancer cell proliferation inhibitory activity
1. Introduction
Currently, there is great interest in the strong biological activity of compounds commonly found in
some foods. Such compounds have been considered safe functional compounds. Polyphenols are found
in various plants such as vegetables and fruits and are consumed regularly [1,2]. Among polyphenol
compounds, proanthocyanidins such as oligomeric flavan-3-ols and condensed tannins are known to
be strongly bioactive compounds. It is widely believed that proanthocyanidins have a beneficial impact
on health; therefore, they are included in various health foods, and scientific investigations of
proanthocyanidins are becoming increasingly important. However, the structure–activity relationship
(SAR) of proanthocyanidins is not well understood because, in many cases, they are obtained as mixtures
of various analogues. Thus, purification of each compound is difficult. Therefore, synthesis is considered
the most suitable method for clarifying the SAR of proanthocyanidins. Following contribution by
Kozukowski et al. [3–5], many studies on proanthocyanidin synthesis and their biological properties
were demonstrated [6–14].
We have also reported a simple, versatile, stereoselective and length controlled synthetic method for
various procyanidins, a member of the proanthocyanidin class, which have two hydroxyl groups on the
B-ring. We demonstrated that galloyl modification of the hydroxyl groups of flavan-3-ols enhanced
their biological activities [15–18]. We further reported the synthesis of semi-acetylated analogues 1–3
of procyanidin B1, a dimeric flavan-3-ol, and discussed their inhibitory activities against HeLa S3 cell
proliferation (Figure 1). The lower-unit acetylated procyanidin B1 (2) strongly inhibited proliferation of
HeLa S3 cells, whereas procyanidin B1 (1) and the upper-unit acetylated analog 3 showed no inhibitory
activity [19]. These results indicated that the upper-unit of dimeric flavan-3-ol is critical for biological
activity. More recently, we elucidated that there is poor correlation between the inhibitory activity of
HeLa S3 cell proliferation and 2,2-diphenyl-l-picrylhydrazyl (DPPH) radical scavenging activity [20].
In addition, we proved that the stereochemistry of the 3-hydroxyl group of flavan-3-ol-3,5-di-O-gallate
is important for inhibitory activity against HeLa S3 cell proliferation [20].

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Figure 1. Structure of procyanidin B1 (1) and semi-acetylated analogues: lower-unit acetylated
B1 (2) and upper-unit acetylated B1 (3).
Here, we describe further investigations. Synthetic studies of dimeric flavan-3-ols, (−)-epigallocatechin-
3-O-gallate (4), (−)-epigallocatechin (5), and (+)-catechin (6) as well as SAR studies were performed
(Figure 2). In addition to the inhibitory activity assay against HeLa S3 cell, antimicrobial activity against
Saccharomyces cerevisiae is also demonstrated. Oligomeric flavan-3-ols consisting of 4, 5 and 6 are
isolated from fermented foods such as beer, wine, etc. Flavan-3-ols and proanthocyanidins are known as
antimicrobial active agents against various microorganisms, including yeast [21]. Although numerous
studies about the biologically effect on yeast of plant extracts which are mixtures of various polyphenol
compounds have been reported [22,23], there is little information allowing a detailed SAR study. Our
results suggested that increases in the number of phenolic hydroxyl groups in the entire molecule correlate
poorly with biological activities. In addition, we confirmed the importance of the upper-unit for the
functionality of dimeric flavan-3-ols.
Figure 2. Structure of principal flavan-3-ols.
2. Results and Discussion
The synthesis targets are shown in Figure 3. We synthesized four dimeric compounds:
(−)-epigallocatechin-[4,8]-(+)-catechin (7), (−)-epigallocatechin-[4,8]-(−)-epigallocatechin (8),
(−)-epigallocatechin-[4,8]-(−)-epigallocatechin-3-O-gallate (9), and (+)-catechin-[4,8]-(−)-epigallocatechin
(10) to determine which part is the most important for biological activities and if the total number of
phenolic hydroxyl groups in the entire molecule correlates with biological properties. The synthesized
compounds 7–10 are natural products that can be isolated from beverages, e.g., green tea [24] and
beer [25], and are known as strongly bioactive polyphenols.

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Figure 3. Synthesized dimeric flavan-3-ols.
Compounds 7–9 were synthesized using an electrophile 13 derived from commercially available
(−)-epigallocatechin-3-O-gallate (EGCG: 4), as shown in Scheme 1. Eight phenolic hydroxyl groups of
4 were protected with benzyl groups. This reaction proceeded smoothly and afforded octa-O-benzylated
EGCG (11) in 66% yield. The galloyl ester moiety of 11 was removed by alkaline hydrolysis to give 12
in quantitative yield. Compound 12 was converted to electrophile 13 by oxidative modification at the
C4 position with DDQ and 2-ethoxyethanol [26].
Scheme 1. Synthesis of EGCG electrophile 13. Reagents and conditions: (a) BnBr, K2CO3,
DMF, 66%; (b) K2CO3, CH2Cl2–MeOH, quant.; (c) 2-ethoxyethanol, DDQ, CH2Cl2, 64%.
The electrophile 13 was condensed with 14, 12, and 11 and gave dimeric compounds 15, 16, and 17
in 56%, 57%, and 40% yields, respectively (Scheme 2). In those condensation reactions, only 3,4-trans
products were obtained stereoselectively without 3-O-modification of electrophile 13. Following HPLC
purification, removal of benzyl groups by hydrogenation with Pd(OH)2/C under H2 atmosphere afforded
7, 8, and 9 as pure products in 23%, 31%, and 21% yields, respectively. We expected that comparison

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between 7 and 8 would show the effect on biological activities induced by the difference in the number
of hydroxyl groups on the lower-unit B-ring and that the galloyl modified 9 could demonstrate whether
the galloyl moiety of the lower-unit is effective to improve activity.
Scheme 2. Synthesis of 7–9. Reagents and conditions: (a) TMSOTf, −20 °C, CH2Cl2,
15: 56%, 16: 57%, 17: 40%; (b) Pd(OH)2/C, H2, THF–MeOH–H2O then HPLC purification,
7: 23%, 8: 31%, 9: 21%.
Synthesis of (+)-catechin-[4,8]-(−)-epigallocatechin (10), a reference compound of 7, is shown in
Scheme 3. The electrophile 18 [27] was condensed with a nucleophile 12 in the presence of TMSOTf as
the Lewis acid at −78 °C in CH2Cl2 and gave dimeric compound 19 in 84% yield. The acetyl group of
19 was removed by DIBAL-H, and deprotection of the benzyl groups by hydrogenation conditions and
HPLC purification afforded compound 10, which had the same building unit as compound 7.
Scheme 3. Synthesis of 10. Reagents and conditions: (a) 12, TMSOTf, CH2Cl2, −78 °C,
84%; (b) DIBAL-H, CH2Cl2, −78 °C, quant.; (c) Pd(OH)2/C, H2, THF–MeOH–H2O (20:1:1)
then HPLC purification, 21%.

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2.1. DPPH Radical Scavenging Activity
Polyphenol compounds are known as extremely strong antioxidants and radical scavengers.
In previous studies, we investigated the DPPH radical scavenging activity of synthesized oligomeric
flavan-3-ols and derivatives [15–18,20]. In Figure 4, DPPH radical scavenging activities of 1, 4, 7–10
at a final concentration of 10, 5, and 1 μM are shown [28]. The SC50 values (the concentration of 50%
scavenging activity) of these compounds were 6.0, 3.3, 4.7, 3.7, 2.9, and 5.9 μM, respectively. These
SC50 values suggested that the number of phenolic hydroxyl groups was no correlated with DPPH radical
scavenging activity. Surprisingly, EGCG (4), non-oligomeric compound, was a more effective radical
scavenger than the oligomeric compounds.
2.2. Antimicrobial Activity against S. cerevisiae
Antimicrobial activity against S. cerevisiae is shown in Figure 5. Upper-unit epigallocatechin
compounds 7 and 8 exhibited strong inhibitory activity against S. cerevisiae proliferation. It should be
noted that activity comparison between compound 7 and reference procyanidin B1 (1) revealed that the
three hydroxyl groups on the B-ring in the upper-unit are very important. Contrary to our expectations,
it is clear from the activity between 8 and 9 that the galloyl moiety at the lower-unit resulted in decreased
activity. Surprisingly, compound 10, which had the same building unit (number of hydroxy groups)
as compound 7, showed no inhibitory activity of proliferation against S. cerevisiae. These data reveal
that the existence of upper-unit hydroxyl groups in the dimeric structure is important for strong activities.
Furthermore, microbial activity against S. cerevisiae showed no correlation to DPPH radical scavenger
ability, which suggests that this antimicrobial activity is due to another mechanism.
100
80
60
10
40
20
0
5
1
VE
1
4
7
8
9
10
Compounds
Figure 4. DPPH radical scavenging activity of synthesized compounds. 1 mL of 30 μM
of DPPH solution in EtOH was added to 1 μL of sample solution in Dimethyl sulfoxide
(DMSO) at final concentrations of 1, 5, and 10 μM (n = 6). Vitamin E was used as a control
compound. Error bars represent standard deviation of the mean (n = 6).

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Figure 5. Antimicrobial activity of synthesized dimeric flavan-3-ols (1, 4, and 7–10) against
S. cerevisiae. Comparison of antimicrobial activity (%) at final concentration of 50 μM after
20 h incubation. 200 μL of culture medium of S. cerevisiae at O.D.600nm 0.25 was treated
with 1 μL of DMSO solution of 1, 4, and 7–10 at final concentrations of 50 μM. DMSO was
used as a negative control. Error bars represent standard deviation of the mean (n = 6).
*** p < 0.001 vs. DMSO-treated groups, asterisks indicate * p < 0.001 in Student’s t test.
2.3. Cervical Epithelioid Carcinoma Cell Line, HeLa S3 Proliferation Inhibitory Activity
The inhibitory activities of the synthetic dimeric flavan-3-ols against HeLa S3 cell proliferation are
shown in Figure 6. Similar to antimicrobial activity against S. cerevisiae, compound 10 showed low
activity relative to other synthesized compounds 7–9. Compared to compound 10, the significance of the
upper epigallocatechin unit of 7 is clear. Furthermore, the huge difference of activity between 1 and 7
indicates the importance of the third hydroxyl group on the B-ring. In addition, this activity was
not enhanced by the introduction of a galloyl moiety to the lower-unit. Among the tested compounds,
compound 7, 8 and 9 showed comparable inhibitory activity, which indicates that the structure of the
lower-unit is not significant.
Figure 6. Inhibitory activity of synthetic dimeric flavan-3-ols against HeLa S3 cell proliferation.
HeLa S3 was incubated with 1 μL of DMSO solution of each compound (final 100 μM)
for 48 h. DMSO was used as a negative control. Error bars represent standard deviation of
the mean (n = 14). *** p < 0.001, ** p < 0.005 vs. DMSO-treated groups, asterisks indicate
* p < 0.001 in Student’s t test.

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3. Experimental Section
3.1. General Information
All commercially available chemicals for chemical synthesis were used without further purification.
All reactions were performed under an argon atmosphere and monitored using thin-layer chromatography
(TLC) with 0.25-mm pre-coated silica gel plates 60F254 (Art 5715, Merck KGaA, Darmstadt, Germany).
An ATAGO (Minato, Japan) AP-300 spectrometer was used to measure optical rotation 1H- and 13C-NMR
spectra (400/100 MHz) were recorded on a DD2 NMR Spectrometer (Agilent, Santa Clara, CA, USA).
A microTOFfocus mass spectrometer (Bruker Daltonics, Billerica, MA, USA) was used to acquire
electrospray ionization (ESI) mass spectra. The human cervical adenocarcinoma cell line, HeLa-S3, and the
normal human fibroblast, WI-38 cell line, was provided by the RIKEN BRC through the National
Bio-Resource Project of the MEXT (Tsukuba, Japan). Synthesized compounds were dissolved in dimethyl
sulfoxide (DMSO) and stored at –25 °C. HPLC purification was carried out on an Ascentis® column
(SUPELCO® analytical, Sigma Aldrich Co., St. Louis, MO, USA; 25 cm × 21.5 mm, 5 μm) using the
solvents (A) 0.05% HCOOH in CH3CN and (B) 0.05% HCOOH and 10% CH3CN in H2O. Elution was
done with a linear gradient 20%–100% B in 20 min (flow rate, 4.0 mL/min).
3.2. Synthesis
(2R,3R)-Octa-O-benzyl-(−)-epigallocatechin 3-O-gallate (11). To a solution of 4 (2.00 g, 4.36 mmol) in
DMF (20.0 mL) was added K2CO3 (6.02 g, 43.6 mmol) and BnBr (6.22 mL, 52.4 mmol) slowly at 0 °C, and
the mixture was stirred for 24 h. The reaction mixture was quenched with H2O and extracted with EtOAc
and the organic phase was washed with brine and dried with MgSO4. Filtration, concentration, and silica
gel column chromatography (n-hexane/EtOAc; 10:1–3:1, 40% CHCl3) afforded 3.39 g of 11 (2.88 mmol,
66%) as a colorless amorphous powder: [α]D24 −76.8 (c 1.12, CHCl3); 1H-NMR (CDCl3) 7.43–7.10 (40H, m),
6.73 (4H, s), 6.40 (1H, s), 6.34 (1H, s), 5.66 (1H, br s), 5.05–4.59 (17H, m), 3.14–3.08 (2H, m); 13C-NMR
(CDCl3) 164.8, 158.9, 158.0, 155.6, 152.9, 152.4, 142.7, 138.4, 137.8, 137.5, 136.9, 136.8, 136.4, 133.2,
128.6–127.2 (×19), 125.0, 109.2, 106.7, 101.0, 94.7, 94.0, 78.0, 75.1, 75.0, 71.2, 71.1, 70.2, 70.0, 68.3,
26.2; ESILRMS (m/z) 1205 (35), 1203 (82), 1202 ([M + Na]+, 100); ESIHRMS calcd. for C78H66O11Na,
1201.4503; found 1201.4514.
(2R,3R)-Penta-O-benzyl-(−)-epigallocatechin (12). To a solution of 11 (5.39 g, 4.57 mmol) in
CH2Cl2/MeOH (1:1) was added K2CO3 (5.05 g, 36.5 mmol) at 0 °C, and the mixture was stirred for 30 min.
The reaction mixture was quenched with saturated NH4Cl solution and extracted with EtOAc. Drying,
filtration, concentration, and silica gel column chromatography (n-hexane/EtOAc; 10:1~2:1, 40% CHCl3)
afforded 3.46 g of 12 (4.57 mmol, quant.) as a colorless amorphous powder: [α]2D4 −21.0 (c 1.38, CHCl3);
1H-NMR (CDCl3) 7.45–7.20 (25H, m), 6.82 (2H, s), 6.29 (2H, s), 5.14 (4H, s), 5.07 (2H, s), 5.02–4.90
(5H, m), 4.22 (1H, br s), 3.01 (1H, d, J = 13.6 Hz), 2.93 (1H, dd, J = 3.6, 13.6 Hz), 1.67 (1H, br);
13C-NMR (CDCl3) 158.3, 158.0, 155.1, 153.0, 138.4, 137.8, 137.0, 136.94, 136.91, 133.7, 128.60, 128.57,
128.53, 128.5 (C × 2), 128.2, 128.0, 127.91, 127.90, 127.8, 127.6 (C × 2), 127.5, 127.2, 106.2, 201.0,
94.7, 94.1, 78.6, 75.2, 71.3, 70.2, 70.0, 66.4, 28.1; ESILRMS (m/z) 781 (14), 780 (51), 779 ([M + Na]+,
100), 758 (2.1), 757 ([M + H]+, 3.7); ESIHRMS calcd. for C50H44O7Na, 779.2985; found 779.2975.

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(2R,3R,4S)-Penta-O-benzyl-4-(2″-ethoxyethoxy)-(−)-epigallocatechin (13). To a solution of 12 (500 mg,
0.661 mmol) and ethoxyethanol (0.639 mL) in CH2Cl2 (35.0 mL) was added slowly DDQ (1.49 equiv.,
223 mg, 0.982 mmol) at 0 °C. After stirring for 5 h at RT, excess of 4-(dimethylamino)pyridine was
added to the solution at 0 °C and the mixture was stirred for 10 min. The resulting purple solid was
removed by filtration and the filtrate was washed with water and brine, and dried (MgSO4). Filtration,
concentration and short silica gel column chromatography (CHCl3) and silica gel column chromatography
(n-hexane/EtOAc; 10:1–3:1, 20% CHCl3) afforded 13 (355 mg, 420 mmol, 64%) as a white foam:
[α]2D8 −5.00 (c 0.40, CHCl3); 1H-NMR (CDCl3) 7.45–7.16 (25H, m), 6.85 (2H, s), 6.29 (1H, s), 6.28 (1H, s),
5.17 (1H, br s), 5.14 (4H, s), 5.06–5.02 (6H, m), 4.61 (1H, br s), 4.03–4.01 (1H, m), 3.85–3.75 (2H, m), 3.51
(2H, t, J = 5.2 Hz), 3.42 (2H, q, J = 6.8 Hz), 1.59−1.56 (1H, m), 1.17 (3H, t, J = 6.8 Hz); 13C-NMR (CDCl3)
160.6, 159.8, 156.1, 153.1 (×2), 138.4, 131.8, 136.9, 136.72, 136.70, 128.6 (×2), 128.5 (×2), 128.2,
128.0, 127.9, 127.8, 127.7, 127.6, 127.5, 106.4, 102.0, 94.4, 94.2, 75.2, 75.0, 71.4, 70.3, 70.1, 69.9, 68.9,
68.6, 66.5, 16.3; ESILRMS (m/z) 869 (19), 868 (62), 867 ([M + Na]+, 100), 846 (3.7), 845 ([M + H]+,
6.8); ESIHRMS calcd. for C54H52O9Na, 867.3509; found 867.3515.
(2R,2″R,3R,3″S,4R)-[4,8]-2,3-cis-3,4-trans-Nona-O-benzyl-(−)-epigallocatechin-(+)-catechin (15). To
a solution of 13 (16.0 mg, 18.9 μmol) and 14 (55.0 mg, 84.5 μmol) in CH2Cl2 (5.0 mL) was added
dropwise TMSOTf (50.0 μL, 25.0 μmol, 0.5 M solution in CH2Cl2) at −20 °C. After stirring for 5 min,
the pale yellow reaction mixture was quenched with saturated NaHCO3. The mixture was extracted
with CHCl3 and the organic phase was washed with water and brine, and dried (MgSO4). Filtration,
concentration, and preparative silica gel TLC purification (hexane/EtOAc, 2.5:1) afforded 15.0 mg
of 15 (10.5 μmol, 56%) as a colorless amorphous powder: [α]2D4 +30.0 (c 0.10, CHCl3); 1H-NMR (CDCl3,
0.77:0.23 mixture of rotational isomers) major isomer: 7.47–6.84 (34.65H, m), 6.80 (0.77H, t, J = 8.0 Hz),
6.73 (0.77H, d, J = 1.4 Hz), 6.50 (0.77H, dd, J = 1.4, 8.1 Hz), 6.37 (0.77H, s), 6.04 (0.77H, d, J = 2.2 Hz),
5.57 (0.77H, d, J = 2.2 Hz), 5.40 (0.77H, s), 5.18–4.91 (13.86H, m), 4.87 (0.77H, d, J = 5.2 Hz), 4.64
(0.77H, d, J = 11.2 Hz), 4.54 (0.77H, d, J = 11.3 Hz), 4.04 (0.77H, m), 3.76 (0.77H, ddd, J = 6.8, 9.2,
9.6 Hz), 3.65 (0.77H, d, J = 9.2 Hz), 3.26 (0.77H, dd, J = 6.6, 16.8 Hz), 2.60 (0.77H, dd, J = 9.6, 16.8 Hz),
1.76 (0.77H, br, s), 1.43 (0.77H, br s); minor isomer: 7.47–6.80 (11.27H, m), 6.24 (0.23H, d, J = 2.2 Hz),
6.21 (0.23H, d, J = 2.3 Hz), 6.10 (0.23H, d, J = 1.9 Hz), 5.31 (0.23H, s), 5.18–4.91 (4.83H, m), 4.42
(0.23H, d, J = 12.2 Hz), 3.61 (0.23H, m), 3.18 (0.23H, dd, J = 5.3, 16.2 Hz), 2.70 (0.23H, dd, J = 9.5,
16.3 Hz), 1.55 (0.46H, br s); 13C-NMR (CDCl3, 0.77:0.23 mixture of rotational isomers) major isomer:
158.2, 157.0, 156.0 (×2), 155.1, 154.6, 153.1 (×2), 149.3, 148.8, 138.3, 138.0, 137.4–137.0 (C × 8),
134.9, 130.3, 128.7–126.8 (C × 24), 120.6, 114.0, 112.1, 111.3, 106.3, 104.4, 104.2, 93.6, 93.1, 91.6,
81.7, 75.3, 72.4, 71.4 (×2), 71.3, 70.7, 70.3, 70.0, 69.6, 69.2, 68.7, 35.6, 29.1; minor isomer: 158.4, 157.8
(×2), 157.0, 156.0, 155.6, 152.8 (×2), 149.4, 149.0, 138.2, 138.0, 137.4–137.0 (C × 8), 134.8, 131.0,
128.7–126.8 (C × 24), 120.2, 115.0, 114.6, 113.0, 106.8, 102.8, 102.3, 94.5, 93.4, 92.8, 81.6, 75.6, 71.5
(×2), 71.3, 71.2 (×2), 71.0, 70.2, 68.4, 68.2, 35.7, 29.7; ESILRMS (m/z) 1431 (15), 1430 (43), 1429
(100), 1428 ([M + Na]+, 91), 1408 (11), 1407 (21), 1406 ([M + H]+, 22), 832 (9.4), 831 (15); ESIHRMS
calcd. for C93H80O13Na, 1427.5497; found 1427.5455.
(2R,2″R,3R,3″'R,4R)-[4,8]-2,3-cis-3,4-trans-Deca-O-benzyl-(−)-epigallocatechin-(−)-epigallocatechin (16).
To a solution of 13 (12.0 mg, 14.2 μmol) and 12 (42.1 mg, 55.7 μmol) in CH2Cl2 (5.0 mL) was added

Molecules 2015, 20
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dropwise TMSOTf (40.0 μL, 20.0 μmol, 0.5 M solution in CH2Cl2) at −20 °C. After stirring for 5 min,
the pale yellow reaction mixture was quenched with saturated NaHCO3. The mixture was extracted
with CHCl3 and the organic phase was washed with water and brine, and dried (MgSO4). Filtration,
concentration, and preparative silica gel TLC purification (hexane/EtOAc, 2:1) afforded 12.4 mg of 16
(8.09 μmol, 57%) as a colorless amorphous powder: [α]2D3 −30.0 (c 0.10, CHCl3) {lit. [26] [α]D23 +16.6
(c 6.35, CHCl3)}; 1H-NMR (CDCl3, 0.75:0.25 mixture of rotational isomers) major isomer: 7.46–6.80
(37.5H, m), 6.89 (1.5H, s), 6.40 (1.5H, s), 6.03 (0.75H, d, J = 2.2 Hz), 5.71 (0.75H, d, J = 2.2 Hz), 5.56
(0.75H, s), 5.31–4.81 (15H, m), 4.68 (0.75H, d, J = 11.2 Hz), 4.52 (0.75H, d, J = 11.2 Hz), 4.08 (0.75H,
br s), 3.98 (0.75H, s), 3.92–3.86 (0.75H, m), 3.01 (0.75H, d, J = 17.9 Hz), 2.90 (0.75H, dd, J = 4.8, 17.9 Hz),
1.77 (0.75H, d, J = 5.3 Hz), 1.51 (0.75H, d, J = 3.3 Hz); minor isomer: 7.46–6.80 (12.75H, m), 6.68
(0.25H, d, J = 1.9 Hz), 6.37 (0.5H, s), 6.26 (0.25H, d, J = 2.3 Hz), 6.21 (0.25H, s), 6.10 (0.25H, d, J = 2.3 Hz),
5.31–4.81 (5H, m), 4.63 (0.25H, d, J = 12.0 Hz), 4.39 (0.25H, d, J = 12.0 Hz), 4.09–3.85 (0.5H, m),
3.60–3.70 (0.25H, m), 3.12–2.98 (0.5H, m), 1.69 (0.25H, d, J = 6.0 Hz), 1.32 (0.25H, d, J = 7.5 Hz);
13C-NMR (CDCl3) 158.3, 158.0, 156.6, 156.0, 155.0, 154.4, 153.1, 152.6, 138.2, 138.0, 138.0, 137.6,
137.4, 137.3, 137.2, 137.17, 137.06, 137.02, 134.7, 133.5, 128.7, 128.6, 128.5, 128.42, 128.38, 128.32,
128.29, 128.14, 128.13, 128.02, 127.9, 127.8, 127.71, 127.70, 127.66, 127.63, 127.60, 127.4, 127.16,
127.14, 127.12, 126.8, 111.3, 106.1, 105.3, 104.3, 102.4, 93.7, 93.4, 91.6, 79.2, 75.8, 75.23, 75.22, 72.6,
71.5, 71.3, 71.1, 70.5, 70.4, 70.1, 70.0, 69.1, 66.4, 35.6, 28.5 (peaks of minor isomer were not identified);
ESILRMS (m/z) 1537 (17), 1536 (52), 1535 ([M + Na]+, 100), 1534 (8), 1512 ([M + H]+, 0.8); ESIHRMS
calcd. for C100H86O14Na, 1533.5915; found 1533.5861.
(2R,2''R,3R,3''R,4R)-[4,8]-2,3-cis-3,4-trans-Trideca-O-benzyl-(−)-epigallocatechin-(−)-epigallo-catechin-
3''-gallate (17). To a solution of 13 (13.0 mg, 15.4 μmol) and 11 (81.3 mg, 69.0 μmol) in CH2Cl2 (5.0 mL)
was added dropwise TMSOTf (40.0 μL, 20.0 μmol, 0.5 μM solution in CH2Cl2) at −20 °C. After stirring for
5 min, the pale yellow reaction mixture was quenched with saturated NaHCO3. The mixture was extracted
with CHCl3 and the organic phase was washed with water and brine, and dried (MgSO4). Filtration,
concentration, and preparative silica gel TLC purification (hexane/EtOAc, 2:1) afforded 12.0 mg of 17
25
1
(6.14 μmol, 40%) as a colorless amorphous powder: [α]D −50.0 (c 0.10, CHCl3); H-NMR (CDCl3)
7.44–6.49 (66.5H, m), 6.94 (1H, s), 6.82 (1H, s), 6.79 (1H, s), 6.49 (1H, s), 6.44 (0.5H, s), 6.24 (0.5H, s),
6.13 (0.5H, br s), 6.12 (0.5H, br s), 6.06 (0.5H, br s), 5.82–5.78 (0.5H, m), 5.75 (0.5H, br s), 5.69 (0.5H, s),
5.43–5.38 (0.5H, m), 5.32–4.20 (25H, m), 4.26–4.22 (0.5H, m), 4.12–4.09 (0.5H, m), 3.36–3.30 (2H, m),
3.24 (0.5H, dd, J = 5.8, 18.3 Hz), 3.01 (0.5H, d, J = 18.3 Hz), 1.85 (0.5H, d, J = 6.4 Hz), 1.73 (0.5H, d,
J = 4.5 Hz); 13C-NMR (CDCl3, 0.5:0.5 mixture of rotational isomers) 165.7, 165.4, 158.5, 158.3, 158.1,
157.2, 156.8, 156.5, 156.3, 156.2, 155.2, 155.0, 154.9, 155.6, 153.1, 153.0, 152.5, 152.4, 152.1, 143.0,
142.4, 138.4, 138.2, 138.04, 138.00, 137.86, 138.84, 137.7, 137.5, 137.4, 137.3, 137.2, 137.1, 137.0,
136.93, 136.91, 136.88, 136.82, 136.7 (×2), 136.6, 136.34, 136.28, 134.5, 134.4, 133.2, 132.7, 130.9,
128.73, 128.69, 128.60, 128.54, 128.47, 128.41, 128.39, 128.36, 128.34, 128.29, 128.28, 128.26, 128.25,
128.20, 128.18, 128.14, 128.10, 128.08, 128.07, 128.05, 128.03, 128.00, 127.96, 127.93, 127.88, 127.85,
127.78, 127.75, 127.72, 127.69, 127.65, 127.62, 127.59, 127.54, 127.46, 127.44, 127.42, 127.39, 127.32,
127.18, 127.17, 125.1, 124.7, 111.9, 111.3, 109.4, 109.0, 106.8, 106.3, 105.7, 104.7, 104.2, 103.1, 101.9,
94.8, 94.1, 93.7, 93.4, 92.4, 92.0, 78.6, 76.0, 75.6, 75.3, 75.2, 75.1, 75.0, 74.0, 72.7, 72.4, 71.5, 71.4,
71.33, 71.26, 71.1, 70.9, 70.73, 70.66, 70.1, 70.0, 69.9, 69.8, 69.3, 69.1, 68.2, 67.9, 37.1, 35.5, 29.7, 26.7

Molecules 2015, 20
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(22 peaks of benzyl groups were not observed); ESILRMS (m/z) 1958 (13), 1957 (18), 1956 ([M + Na]+,
13), 1351 (17), 1350 (25), 1235 (17), 1234 (24), 1187 (19), 1186 (32), 1177 (19), 1176 (26), 1143 (23),
1142 (40), 1099 (34), 1098 (58), 1011 (47), 1010 (100); ESIHRMS calcd. for C128H108O18Na, 1955.7433;
found 1955.7427.
(2R,2''R,3R,3''S,4R)-[4,8]-2,3-cis-3,4-trans-(−)-Epigallocatechin-(+)-catechin (7). A solution of 15
(110 mg, 77.1 μmol) in THF/MeOH/H2O (20:1:1, 22 mL) was added to the mixture and hydrogenated
over 20% Pd(OH)2/C (5 mg) under H2 atmosphere for 12 h at RT. Filtration and concentration afforded
a pale brown solid, which was purified with HPLC purification to give 10.4 mg of pure 7 (17.5 μmol,
23%) as a pale brown powder: [α]2D4 +67.3 (c 0.033, MeOH) {lit. [26] [α]2D2 +24.0 (c 0.50, MeOH)};
1H-NMR (CD3OD, −55 °C) 6.88 (1H, d, J = 8.6 Hz), 6.79 (1H, s), 6.67 (1H, d, J = 8.2 Hz), 6.35 (2H, s),
5.89 (1H, d, J = 2.2 Hz), 5.87 (1H, s), 5.80 (1H, s), 5.03 (1H, s), 4.99 (1H, d, J = 4.3 Hz), 4.64 (1H, s),
4.22–4.14 (1H, m), 3.93 (1H, s), 2.63–2.42 (2H, m); 13C-NMR (CD3OD, −55 °C) 158.5, 157.7, 156.4,
155.7, 153.6, 146.3, 146.0, 145.6, 132.9, 132.1, 131.8, 119.0, 115.8, 113.4, 107.6, 106.3, 106.1, 102.3,
99.6, 96.4, 95.4, 94.6, 81.2, 76.8, 72.8, 67.9, 36.5, 26.0; ESILRMS (m/z) 619 (13), 618 (48), 617
([M + Na]+, 100), 597 (11), 596 (38), 595 ([M + H]+, 81); ESIHRMS calcd. for C30H27O13, 595.1452;
found 595.1441.
(2R,2''R,3R,3''R,4R)-[4,8]-2,3-cis-3,4-trans-(−)-Epigallocatechin-(−)-epigallocatechin (8). A solution of
16 (95.0 mg, 61.9 μmol) in THF/MeOH/H2O (20:1:1, 22 mL) was added to the mixture and hydrogenated
over 20% Pd(OH)2/C (5 mg) under H2 atmosphere for 12 h at RT. Filtration and concentration afforded
a pale brown solid, which was purified with HPLC purification to give 11.7 mg of pure 8 (19.1 μmol,
31%) as a pale brown powder: [α]2D4 +10.3 (c 0.29, MeOH) {lit. [26] [α]2D2 +24.0 (c 0.50, MeOH)};
1H-NMR (CD3OD, −55 °C) 6.58 (2H, s), 6.30 (2H, s), 5.85 (1H, s), 5.55–5.40 (2H, m), 4.96 (1H, s),
4.86 (1H, s), 4.59 (1H, s), 4.24 (1H, br s), 3.78 (1H, s), 2.92 (1H, d, J = 16.5 Hz), 2.79 (1H, d, J = 16.5 Hz);
13C-NMR (CD3OD, −55 °C) 157.8, 157.7, 157.5, 156.65, 156.56, 154.5, 146.5, 146.3, 133.04, 133.02,
131.5, 131.1, 107.0, 106.1, 105.9, 102.1, 99.4, 96.5, 95.5, 95.2, 79.2, 76.8, 73.5, 66.9, 36.5, 30.2;
ESILRMS (m/z) 634 (18), 633 ([M + Na]+, 55), 613 (7.8), 612 (32), 611 ([M + H]+, 100); ESIHRMS
calcd. for C30H27O14, 611.1401; found 611.1356.
(2R,2''R,3R,3''R,4R)-[4,8]-2,3-cis-3,4-trans-(−)-Epigallocatechin-(−)-epigallocatechin 3''-O-gallate (9).
A solution of 17 (150 mg, 76.0 μmol) in THF/MeOH/H2O (20:1:1, 22 mL) was added to the mixture and
hydrogenated over 20% Pd(OH)2/C (5 mg) under H2 atmosphere for 12 h at RT. Filtration and
concentration afforded a pale brown solid, which was purified with HPLC purification to give 12.1 mg
24
1
of pure 6 (15.9 μmol, 21%) as a pale brown powder: [α]D −64.3 (c 0.14, MeOH); H-NMR (CD3OD,
−55 °C) 7.00 (2H, s), 6.60 (2H, s), 6.41 (2H, s), 6.00 (1H, s), 5.96 (1H, s), 5.88 (1H, s), 5.59 (1H, br s),
5.12 (1H, br s), 5.07 (1H, br s), 4.79 (1H, br s), 3.91 (1H, br s), 3.04 (1H, d, J = 15.8 Hz), 2.89 (1H, d,
13
J = 15.8 Hz); C-NMR (CD3OD) 167.6, 158.2, 157.8, 157.7, 156.5, 155.8, 154.4, 146.4 (×2), 146.0,
139.6, 133.3, 133.1, 131.7, 130.5, 121.0, 109.9, 107.9, 106.2, 106.1, 102.2, 99.0, 96.6, 95.6, 95.2, 78.1,
77.0, 73.6, 69.3, 36.5, 26.8; ESILRMS (m/z) 787 (7.5), 786 (24), 785 ([M + Na]+, 53), 765 (13), 764
(44), 763 ([M + H]+, 100); ESIHRMS calcd. for C37H31O18, 763.1510; found 763.1532.

Molecules 2015, 20
18881
(2R,2''R,3S,3''R,4S)-[4,8]-2,3-trans-3,4-trans-3-Acetoxy-nona-O-benzyl-(+)-catechin-(−)-epi-gallocatechin
(19). To a solution of 18 (281 mg, 360 μmol) and 12 (959 mg, 1.44 mmol) in CH2Cl2 (30 mL) was added
dropwise TMSOTf (432 μL, 432 μmol, 1.0 M solution in CH2Cl2) at −78 °C. After stirring for 5 min, the
pale yellow reaction mixture was quenched with saturated NaHCO3. The mixture was extracted with CHCl3
and the organic phase was washed with water and brine, and dried (MgSO4). Filtration, concentration,
and silica gel column chromatography (n-hexane/EtOAc; 8:1–2:1, 40% CHCl3) afforded 464 mg of 12
23
1
(302 μmol, 84%) of as a colorless amorphous powder: [α]D −26.7 (c 0.6, CHCl3); H-NMR (CDCl3,
0.83:0.13 mixture of rotational isomers) major isomer: 7.47–6.77 (35.69H, m), 6.91 (0.83H, d, J = 1.8 Hz),
6.73 (1.66H, s), 6.62 (0.83H, dd, J = 1.8, 8.0 Hz), 6.23 (0.83H, d, J = 2.4 Hz), 6.22 (0.83H, s), 6.14
(0.83H, d, J = 2.4H Hz), 5.82 (0.83H, t, J = 9.6 Hz), 5.18–4.82 (14.11H, m), 4.68 (0.83H, d, J = 9.6 Hz),
4.61 (0.83H, d, J = 10.2 Hz), 4.48 (0.83H, d, J = 10.2 Hz), 3.98–3.92 (0.83H, m), 3.57 (0.83H, s), 2.82
(0.83H, d, J = 17.2 Hz), 2.55 (0.83H, dd, J = 4.4, 17.2 Hz), 1.60 (2.49H, s), 1.51–1.50 (0.83H, m); minor
isomer: 7.47–6.61 (5.98H, m), 6.62 (0.26H, s), 6.19 (0.13H, d, J = 2.4 Hz), 6.12 (0.13H, d, J = 2.4 Hz),
6.06 (0.13H, t, J = 2.4 Hz), 5.92 (0.13H, s), 5.18–4.47 (2.99H, m), 2.98–2.95 (0.26H, m), 1.50 (0.39H, s),
13
a proton of hydroxyl group was not identified; C-NMR (CDCl3) 169.1, 158.2, 157.8, 156.6, 156.2,
155.8, 152.9, 149.1, 149.0, 137.9, 137.4, 137.3, 137.2, 137.0, 136.5, 128.7, 128.6, 128.4, 128.3, 128.2,
128.1, 128.0, 127.8, 127.7, 127.5, 127.3, 127.0, 121.3, 114.6, 113.6, 110.8, 108.3, 105.8, 100.2, 94.8,
94.3, 91.3, 80.4, 75.3, 73.0, 71.7, 71.3, 71.2, 71.0, 70.2, 70.0, 66.0, 35.2, 28.3, 20.7; ESILRMS (m/z);
1488 (42.8), 1487 (71.7), 1486 (67.2), 1472 (52.7), 1471([M + Na]+, 100), 1470 (94.3), 1449 (4.8), 1448
([M + H]+, 4.8); ESIHRMS calcd. for C95H83O14, 1447.5783; found 1447.5777.
(2R,2''R,3S,3''R,4S)-[4,8]-2,3-trans-3,4-trans-Nona-O-benzyl-(+)-catechin-(−)-epigallocatechin (20). To
a solution of 19 (184 mg, 127 μmol) in CH2Cl2 (20 mL) was added dropwise DIBAL–H (1.31 mL,
1.31 mmol, 1.0 M solution in hexane) at −78 °C. After stirring for 30 min at RT, the pale yellow reaction
mixture was quenched with saturated Rochelle salt. The mixture was extracted with CHCl3 and the organic
phase was washed with brine, and dried (MgSO4). Filtration, concentration, and preparative silica gel TLC
purification (hexane/EtOAc, 2:1) afforded 176 mg of 20 (125 μmol, 99%) as a colorless amorphous
powder: [α]2D3 −70.0 (c 2.06, CHCl3); 1H-NMR (CDCl3) 7.50–6.60 (50H, m), 6.22 (1H, s), 6.20 (1H, d,
J = 2.8 Hz), 6.17 (1H, d, J = 2.8 Hz), 5.18–4.45 (20H, m), 4.28 (1H, t, J = 9.2 Hz), 3.91 (1H, br s), 3.77
(1H, s), 2.97 (1H, d, J = 17.3 Hz), 2.57 (1H, dd, J = 4.28, 17.3 Hz), a proton of hydroxyl group was not
identified; 13C-NMR (CDCl3) 158.0, 157.8, 156.8, 156.1, 155.5, 153.3, 152.9 (×2), 149.3, 149.1, 138.0,
137.8, 137.4, 137.3, 137.2, 137.15 (×2), 137.08 (×2), 136.7, 134.3, 131.6, 128.6–127.1 (30C), 121.3,
114.8, 113.3, 111.9, 108.8, 105.6, 100.5, 94.9, 94.2, 91.4, 82.3, 75.2, 73.2, 71.3, 71.14 (×2), 71.06, 70.23,
70.16, 70.04, 69.98, 66.0, 36.9, 27.9; ESILRMS (m/z); 1446 (30.0), 1445 (47.6), 1444 (47.3), 1430 (41.0),
1429 ([M + Na]+, 77.4), 1428 (73.5), 1407 (12.3), 1406 ([M + H]+,16.8), 1405 (13.4); ESIHRMS calcd.
for C93H81O13, 1405.5677; found 1405.5672.
(2R,2''R,3S,3''R,4S)-[4,8]-2,3-trans-3,4-trans-(+)-Catechin-(−)-epigallocatechin (10). A solution of 20
(140 mg, 99.6 μmol) in THF/MeOH/H2O (20:1:1, 22 mL) was added to the mixture and hydrogenated
over 20% Pd(OH)2/C (5 mg) under H2 atmosphere for 12 h at RT. Filtration and concentration afforded
a pale brown solid, which was purified with HPLC purification to give 12.6 mg of pure 10 (21.2 μmol,
21%) as a pale brown powder: [α]2D3 −138 (c 0.116, acetone) {lit. [29] [α]D17 −168.9 (c = 1.0, acetone)};

Molecules 2015, 20
18882
1H-NMR (CD3OD, 0.91:0.09 mixture of rotational isomers) major isomer: 7.04 (0.91H, d, J = 1.7 Hz),
6.90 (0.91H, dd, J = 1.7, 8.1 Hz), 6.85 (0.91H, d, J = 8.2 Hz), 6.73 (1.82H, s), 6.08 (0.91H, s), 5.88
(1.82H, s), 4.92 (0.91H, br s), 4.71 (0.91H, d, J = 7.7 Hz), 4.61 (0.91H, dd, J = 7.7, 10.6 Hz), 4.46 (0.91H,
d, J = 10.6 Hz), 4.31–4.27 (0.91H, m), 2.93 (0.91H, dd, J = 4.1, 21.0 Hz), 2.85 (0.91H, d, J = 16.5 Hz);
minor isomer: 6.79 (0.09H, d, J = 2.0 Hz), 6.70 (0.09H, d, J = 8.7 Hz), 6.59 (0.18H, s), 6.49 (0.09H, dd,
J = 1.8, 8.4 Hz), 4.35 (0.09H, d, J = 4.7 Hz), 4.25 (0.09H, d, J = 6.6 Hz), 2.75–2.69 (0.18H, m), a proton of
hydroxyl group was not identified; 13C-NMR (CDCl3) 177.5, 176.2, 175.0, 174.5, 173.9, 165.4, 165.0,
164.6, 152.0, 151.4, 150.6, 139.9, 135.3, 135.0, 126.9, 125.7, 118.3, 116.6, 116.5, 115.3, 102.5, 98.80,
92.52, 86.06, 57.35, 19.29; ESILRMS (m/z); 634 (7.8), 633 (23), 619 (10), 618 (42), 617 ([M + Na]+,
100), 595 ([M + H]+, 3.1); ESIHRMS calcd. for C30H27O13, 595.1452; found 595.1446.
3.3. DPPH Radical Scavenging Activity
DPPH radical scavenging activity was measured with general procedure [28]. A solution of DPPH
radical in EtOH (30 μM, 1.0 mL) was added to 1 μL of the synthesized each compound in DMSO, and
incubated at 30 °C for 30 min (n = 6). The scavenging activity was estimated with a microplate reader
(Filter Max F5 multi-mode microplate reader; Molecular Devices, Downingtown, PA, USA) to measure
the OD at 515 nm. Negative controls, the samples that 1 μL of DMSO added to the 1.0 mL of EtOH
were prepared at the same time. And the absorbance values converted into the percentage radical
scavenging activity as follows: [(absorbance of the control − absorbance of the sample)/absorbance of
the control] × 100. VE (vitamin E) was used as the standard sample.
3.4. Antimicrobial Activity against S. cerevisiae
Overnight culture of S. cerevisiae was diluted to OD = 0.25 with culture medium, and 200 μL of aliquot
was transferred to each well in a 96-well plate. Then 1 μL of the DMSO solution of compound was added
in each well to a final concentration of 50 μM, and the growth of the S. cerevisiae at 27 °C with constant
shaking was monitored by measuring absorbance at 600 nm every 15 min using an incubation reader, HiTS
(Scinics, Itabashi, Japan). The results were obtained as growth curves as well as raw data. DMSO was used
as negative controls. As negative controls, medium alone well and DMSO added to the well were prepared
at the same time.
3.5. Inhibitory Activity of HeLa S3 Cell Proliferation
104 cells per well with 100 μL of medium in a 37 °C incubator equilibrated with a 5% CO2: 95%
humidified air atmosphere. D-MEM (Dulbecco’s Modified Eagle’s Medium; Gibco® (Life Technologies,
Grand Island, NY, USA) supplemented with 5% fetal calf serum and 1% Pen-Strep; Invitrogen™ (Life
Technologies). After 24 h of incubation, 1 μL of synthesized six compounds in DMSO were added (final
100 μM) and incubated for 48 h. As negative controls, medium alone well and DMSO added to the well
were prepared at the same time. After the medium was removed and the cell was washed with PBS, 90 μL
of new medium and 10 µL of the MTT solution (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide, 5 mg/mL) was added to each well and incubated at 37 °C for 2.5 h. After incubation the reaction
medium was removed and 100 μL of DMSO was added to each well and mix thoroughly with the pipette.

Molecules 2015, 20
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Following which, viable cells were assessed using a microplate reader (Filter Max F5 multi-mode microplate
reader; Molecular Devices) to measure the OD at 570 nm.
4. Conclusions
In conclusion, we have synthesized four dimeric flavan-3-ol derivatives to clarify the importance of
the upper-unit for biological activities. Our results support the assumption that the hydroxyl groups on the
B-ring of the upper-unit are essential for the antimicrobial activity against S. cerevisiae and HeLa S3 cell
proliferation inhibitory activity. Furthermore the correlation of antimicrobial activity against S. cerevisiae,
proliferation inhibitory activity on HeLa S3 cells and of 2,2-diphenyl-l-picrylhydrazyl radical scavenging
activity with the number of phenolic hydroxyl groups was low. Further synthesis and biological works to
explain these mechanisms are now underway, since much more research using various analogs is needed
to completely understand the SAR of these compounds.
Acknowledgments
This work was supported by BRAIN: Program for Promotion of Basic and Applied Research for
Innovations in Bio-oriented Industry (A. Saito), Technology Research Promotion Program for Agriculture,
Forestry, Fisheries, and Food Industry (A. Saito), the Tojuro Iijima Foundation for Food and Technology
(A. Saito), the Kurata Memorial Hitachi Science and Technology Foundation (A. Saito), JSPS KAKENHI
Grant Number 24590014 (Grants-in-Aid for Scientific Research (C), N. Nakajima).
Author Contributions
Y.H. and S.T. synthesized all compounds for biological assays; Y.A. and S.O. measured the inhibitory
activity against HeLa S3 cells proliferation; M.T., S.D. and M.I. measured the antimicrobial activity;
T.K. measured the DPPH scavenging activity; M.H. measured MS spectrometry; T.K., N.N. and A.S.
supervised the study; and Y.H. and A.S. wrote the manuscript.
Conflicts of Interest
The authors declare no conflict of interest.
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Sample Availability: Samples of the compounds 1–3 and 7–10 are available from the authors.
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