Magnetic Resonance in Chemistry p. 1 - 3 (1988)
Update date:2022-08-03
Dowd, Diane
Gettins, Peter
To test the feasibility of using 77Se NMR to distinguish between different proposed mechanisms of action of the selenoenzyme glutathione peroxidase, model compounds containing the proposed selenium moieties have been synthesized and their 77Se NMR spectra recorded.It was found that the difference in chemical shift between the two proposed resting states, selenol or selenenic acid, is approximately 1300 ppm.For the next step, oxidation by hydrogen peroxide, a much smaller difference of about 100 ppm was found between selenenic and seleninic acid species.The products of the second reaction step, selenide sulfide vs selenoxide sulfide, differed in chemical shift by about 470 ppm.It was concluded that, for two of the three states of the enzyme, 77Se NMR could, in principle, be used unambiguously to distinguish between the two proposed mechanisms.KEY WORDS: 77Se NMR; glutathione peroxidase selenoenzyme; selenium model compounds.
View MoreShanghai Hohance Chemical Co., ltd
Contact:13914753421
Address:Fl.5;Bld. 70, Lane 1500; Xinfei Road
Contact:86-10-62983737; +86-10-51287608
Address:4/F Building C, 2 Shangdi Xinxi Road
Shenzhen Sunrising Industry Co., ltd.
Contact:+86 755 86571158 / 86571159 / 86571160
Address:2108 ZHENYE INT. BUSINESS CENTER,NO.3101-90 QIANHAI RD, NANSHAN,SHENZHEN, CHINA
Puyang Huicheng Electronic Material Co., Ltd
website:http://huichengchem.weba.testwebsite.cn/index_en.html
Contact:+86-393-8910800
Address:West Section Shengli Road, Puyang457000, China
Daicel Chiral Technologies (China)CO.,LTD
Contact:021-5046-0086*8
Address:Part C, FL 5, the 16th Building, No. 69, XiYa Road, WaiGaoQiao Free Trade Zone, Shanghai, 200131, P.R.China
Doi:10.1021/ja035782l
(2003)Doi:10.1007/PL00004053
()Doi:10.1248/cpb.30.3133
(1982)Doi:10.1021/j100051a010
(1995)Doi:10.1021/jo00346a043
(1982)Doi:10.1016/S0008-6215(98)00112-8
(1998)