Magnetic Resonance in Chemistry p. 1 - 3 (1988)
Update date:2022-08-03
Dowd, Diane
Gettins, Peter
To test the feasibility of using 77Se NMR to distinguish between different proposed mechanisms of action of the selenoenzyme glutathione peroxidase, model compounds containing the proposed selenium moieties have been synthesized and their 77Se NMR spectra recorded.It was found that the difference in chemical shift between the two proposed resting states, selenol or selenenic acid, is approximately 1300 ppm.For the next step, oxidation by hydrogen peroxide, a much smaller difference of about 100 ppm was found between selenenic and seleninic acid species.The products of the second reaction step, selenide sulfide vs selenoxide sulfide, differed in chemical shift by about 470 ppm.It was concluded that, for two of the three states of the enzyme, 77Se NMR could, in principle, be used unambiguously to distinguish between the two proposed mechanisms.KEY WORDS: 77Se NMR; glutathione peroxidase selenoenzyme; selenium model compounds.
View MoreLanzhou huibang biological chemical technology Co., LTD
Contact:0931-7843964
Address:NO.2011,Yannan Road,Chengguan,
Shandong Xinke Petrochemical Co., Ltd.
Contact:+86-546-7277016
Address:Gudao Industrial Park, Hekou District, Dongying, Shandong Province, China
Hebei Kangtai Pharmaceutical Co.,Ltd
Contact:+86-0317-3512963
Address:Wugang Road,Mengcun of Cangzhou City,Hebei Province ,China
Shanghai Demand Chemical Co., ltd,China
Contact:86-021-55237578/55239122
Address:Room 3H, No.578, Yingkou Road, Yangpu District, Shanghai, China
SHUNYUANSHENG BIO-PHARMTECH CO., LTD
website:https://www.whsysbio.com
Contact:--
Address:Building 13, Liandong U Valley-Wuhan Economic Innovation Valley, No. 259, Xingsan Road, Shamao Street, Hannan District, Wuhan City, Hubei Province
Doi:10.1021/ja035782l
(2003)Doi:10.1007/PL00004053
()Doi:10.1248/cpb.30.3133
(1982)Doi:10.1021/j100051a010
(1995)Doi:10.1021/jo00346a043
(1982)Doi:10.1016/S0008-6215(98)00112-8
(1998)