The Ion-exchange Chromatography of Imino Derivatives of Glycine

Article abstract of DOI:10.1246/bcsj.56.792

The resolution of the six imino derivatives of glycine (Gly) by ion-exchange chromatography is described.The imino compounds included iminodiacetonitrile (1), iminodiacetamide (2), iminodiacetic acid (3), α-(cyanomethylamino)acetamide (4), α-(cyanomethylamino)acetic acid (5), and α-(carbamoylmethylamino)acetic acid (6).A mixture of 1-6 was chromatographed along with Gly, glycinamide, aminoacetonitrile, and NH3 with an automatic amino acid analyzer using Aminex A-4 resin column (0.25φ x 50 cm) and sodium citrate buffers.When the initial buffer of pH 3.25 was changed to pH 6.50 15 min after beginning the analysis, these ten components were completely resolved.The analysis was completed in about 4.5 h.The stability of 1, 4, and 5 in aqueous media at room temperature was also studied.