Synthesis of α-hydroxyacetosyringone

Article abstract of DOI:10.3390/51201287

A phytoalexin from papaya fruit has been synthesized in four steps; this procedure involved a Pummerer- type reaction.

Full text of DOI:10.3390/51201287

Molecules 2000, 5, 1287–1290
molecules
ISSN 1420-3049
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Synthesis of α-Hydroxyacetosyringone
Fernando Echeverri*, Winston Quiñones, Fernando Torres, Mario Duque and Rosendo
Archbold
Department of Chemistry, Universidad de Antioquia, P. O. Box 1226. Medellín, Colombia,
Tel. (57+4)2105658, Fax: (57+4)2330120
*Author to whom correspondence should be addressed; E-mail: echeveri@catios.udea.edu.co
Received: 25 May 2000 / Accepted: 1 November 2000 / Published: 19 December 2000
Abstract: A phytoalexin from papaya fruit has been synthesized in four steps; this
procedure involved a Pummerer- type reaction.
Keywords: Phytoalexin; syringone derivative; Agrobacterium transformation; synthesis;
Pummerer reaction.
Introduction
From papaya slices treated with copper salt or infected with Collectotrichum gloesporioides we
isolated α-hydroxyacetosyringone as a phytoalexin [1,2]; recently, this compound was involved in
plant-pathogen interactions, e.g. it is the major virulence gene activating factor and promotes high
efficiency transformation of Arabidopsis thaliana explants by Agrobacterium tumefaciens [3,4].
Although its synthesis has been reported previously [5], we report here a four-steps synthesis of this
compound
Results and Discussion
We attempted to synthesize α-hydroxyacetosyringone through a sequence of reactions including a
Pummerer reaction. According to Russell and Becker [6,7], aromatic β-ketosulphoxides can be used to
extend the carbon chain and also to add the desired new functional group. These β-ketosulphoxides
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are produced by reaction between DMSO and aldehydes or ketones in basic solutions and oxidation of
the respective β-hydroxysulfoxide intermediate with MnO2 .
Scheme 1. Reactions involved in acetosyringone synthesis.
However, β-hydroxysulfoxides can yielded α,β-unsaturated sulfoxides under acidic conditions or
upon neutralization of the condensation reaction. Moreover, other unexpected compounds have been
isolated too, and their formation involved addition of water to α,β-unsaturated sulfoxide in an acid-
catalyzed process [8]. Surprisingly, α-hydroxyacetosyringone was directly produced, via attack of two
water molecules and thiomethanol elimination in a pH-dependent sequence of reactions.
Thus, when this coupling reaction was carried out between 4-O-benzyl-syringaldehyde and DMSO
product 2 was obtained. This product can be transformed into 3 through a Pummerer-type reaction
(Scheme 1); treatment of this substance with concentrated hydrochloric acid during 5 hours yielded a
mixture of compounds, including compound 3. After hydrogenation of 3 on Pd/C the desired product 4
was obtained (<10% yield from 2). Its spectroscopic data were identical to those α-hydroxy
acetosyringone obtained from natural sources.

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Experimental
General
1
13
NMR spectra were recorded with a Bruker AMX III (at 300 MHz for H and 75.0 MHz for C).
All NMR spectra were taken in CDCl3; MS on a VG Micromass ZAB-2F at 70 eV; IR spectra were
registered with a Perkin Elmer 1600 (FTIR).. TLC and column chromatographies were run using
Merck silica gel and, unless otherwise specified, developed with n-hexane-ethyl acetate (9:1, v/v)
4-Benzyloxysyringaldehyde (1)
The commercial compound 3,5-dimethoxy-4-hydroxy-benzaldehyde (syringaldehyde) (91.09 mg,
0.5 mmol) in MeOH (5 mL) was treated with benzyl chloride (0.12 mL, 1.0 mmol) and stirred for
1
8hours at room temperature. Compound 1 was purified by silica gel column. HRMS: 272.1049. H
NMR: 3.91 (6H, s, x 2-OCH3), 5.14 (2H, s, ArCH2O-), 7.12 (2H, s, H-3, H-5), 7.35 (3H, m, Ar), 7.47
(2H, d, J=6.1, Ar), 9.78 (1H, s, CHO). 13C NMR: 53.89 (q, - OCH3), 75.69 (t, -O-CH2), 107.31 (d, C-3,
C-5), 129.08 (s, C-1), 143.01 (s, C-2,C-6), 154.63 (s, C-4), 191.83 (s, CHO).
3’,5’-Dimethoxy-4’-benzyloxyethenylmethylsulfoxide (2)
A solution of t-BuOK (179.55 mg, 1.6 mmol) in DMSO (5.0 mL) was added to 4-O-benzyl-
syringaldehyde (272.30 mg, 1.0 mmol) 1, in DMSO (3.0 mL) under argon and stirred overnight. After
extraction with ethyl acetate and purification by column chromatography, compound 2 was obtained
o
1
(199.45 mg, 60%). TLC: Rf = 0.35; mp 102-104 C. HRMS: 332.1082. H NMR 300 MHz (δ ppm
CDCl3) 2.70 (3H, s, S–CH3), 3.83 (6H, s, –OMe), 5.02 (2H, s, ArCH2O-), 6.67 (2H, s, H-2’ and H-6’),
6.80 (1H, d, 14.0 Hz, =CH–S), 7.14 (1H, d, 14.0 Hz, Ar–CH=), 7.28-7.36 (3H, m, Ar), 7.46 (2H, dd,
13
2.0 and 7.0 Hz, Ar); C NMR 75.46 MHz: 41.01 (q, S-CH3), 56.10 (q, O-CH3), 75.06 (t, -ArCH2O-),
104.70 (d, C-2, C-5), 129.14 (d, -S-C=CH), 128.15 (d, Bz), 128.44 (s, C-1), 136.51 (d, S-CH=C),
137.41 (s, C-1’), 138.31 (s, C-4), 153.71 (s, C-3, C-5).
4’-O-benzyl-α-hydroxyacetosyringone (3)
The sulfoxide 2 (166.21 mg, 0.5 mmol) was added to 10% HCl (10 mL) and heated to reflux for
five hours. The crude reaction mixture was purified by TLC (silicagel; n-hexane-ethyl acetate 6:1, v/v)
1
and product 3 was thus obtained as a yellow oil (15.11 mg, 10%). HRMS: 302.1154. H NMR : 3.68
(6H, s, x 2 OCH3), 5.07 (2H, s, -COCH2OH), 5.08 (2H, s, Ar-OCH2-), 6.48 (1H, s, -OH), 6.90 (2H, s,
13
H-2’ and H-6’), 7.30 (5H, m, benzyl); C NMR: 56.49 (q, x 2 OCH3), 75.39 (t, x 2CH2), 107.40 (d,
C-2’ and C-5’ ), 128.80 (d, Bz), 132. 90 (s, C-1’), 134.80 (s, C-1’’), 138.87 (s, C-4’), 153.45 (s, C-3’,
C-5’), 192.15 (s, CO).

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α-Hydroxyacetosyringone (4)
Compound 3 (15.11 mg, 0.05 mmol) in MeOH (5 mL) was hydrogenated at room temperature for 5
hours over Pd/C and after workup compound 4 was recovered (10 mg, yield 95%). Its spectroscopical
and physical properties were compared to an authentic sample obtained from papaya slices. mp. 145 º,
1
Rf = 0.28 (n-hexane-ethyl acetate 2:3). HRMS: 212.0685. H NMR : 3.25 (H, s, 2-OH), 3.89 (6H, s,
3’,5’-OCH3), 4.93 (2 H, d, J=1.9, -COCH2OH), 6.34 (H, t, J=1.9, 4-OH), 7.28 (2H, s. H-2’ and H-6);
13C NMR: 54.95 (q, x 2 OCH3), 66.3 (t, C-2), 106.2 (d, C-2), 125.1 (s, C-1), 141.3 (s, C-4), 146.6(s, C-
3, C-5), 196.4 (s, C-1).
Acknowledgements
Author thanks to COLCIENCIAS (Colombia) and Universidad de Antioquia for financial support.
References
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Sample Availability: Samples are available from the authors.
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