Purification and Characterization of Ethyl 2-Methyl-3-oxobutanoate Reductase from Klebsiella pneumoniae IFO3319

Article abstract of DOI:10.1271/bbb.60.760

An enzyme that catalyzes a reduction of ethyl 2-methyl-3-oxobutanoate (1) to ethyl (2R,3S) 3-hydroxy-2-methylbutanoate was found in Klebsiella pneumoniae IFO 3319 cells. The enzyme was isolated from the cells and purified 250-fold by ammonium sulfate frac

Full text of DOI:10.1271/bbb.60.760

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Bioscience, Biotechnology, and Biochemistry
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Purification and Characterization of Ethyl 2-
Methyl-3-oxobutanoate Reductase from Klebsiella
pneumoniae IF03319
Hiroyuki Miyaa, Mitsuru Kawadaa & Yoshio Sugiyamaa
a Integrated Technology Laboratories, Takeda Chemical Industries Ltd., Wadai 10,
Tsukuba, Ibaraki 300–42, Japan
Published online: 12 Jun 2014.
To cite this article: Hiroyuki Miya, Mitsuru Kawada & Yoshio Sugiyama (1996) Purification and Characterization of Ethyl 2-
Methyl-3-oxobutanoate Reductase from Klebsiella pneumoniae IF03319, Bioscience, Biotechnology, and Biochemistry, 60:5,
760-764, DOI: 10.1271/bbb.60.760
To link to this article: http://dx.doi.org/10.1271/bbb.60.760
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