Preparation and antigenic properties of methyl 3β-hydroxy-19-oxo-5-cholen-24-oyl-glycinate 19-(O-carboxymethyl)oxime-bovine serum albumin conjugate

DOI: 10.1248/cpb.32.3088

Source and publish data:

Chemical and Pharmaceutical Bulletin p. 3088 - 3092 (1984)

Update date:2022-09-26

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Authors:

Yamauchi

Kojima Kojima

Nakayama

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Article abstract of DOI:10.1248/cpb.32.3088

The preparation and antigenic properties of 3β-hydroxy-5-cholen-24-oyl-glycine-bovine serum albumin (BSA) conjugate in which the hapten is linked to the carrier protein through an (O-carboxymethyl)oxime bridge at the C-19 position on the steroid portion are described. Antibody raised against the antigen in rabbits possessed high titer and specificity to 3β-hydroxy-5-cholen-24-oyl-glycine, exhibiting no significant cross-reactions with various bile acids.

Full text of DOI:10.1248/cpb.32.3088

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