Chemical and Pharmaceutical Bulletin p. 3088 - 3092 (1984)
Update date:2022-09-26
Topics:
Yamauchi
Kojima
Nakayama
The preparation and antigenic properties of 3β-hydroxy-5-cholen-24-oyl-glycine-bovine serum albumin (BSA) conjugate in which the hapten is linked to the carrier protein through an (O-carboxymethyl)oxime bridge at the C-19 position on the steroid portion are described. Antibody raised against the antigen in rabbits possessed high titer and specificity to 3β-hydroxy-5-cholen-24-oyl-glycine, exhibiting no significant cross-reactions with various bile acids.
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