26
BRUCE L. MARTIN
class of catalytic RNA recently was shown to be activated by Co(NH3)63+ (42) with the
authors concluding that exogenous metal ion served to orient the active site and sta-
bilize the reaction intermediate. Using outer sphere coordination, as does calcineurin,
the action of exogenous metal ion in this reaction is likely directed by the interaction
with substrate. It is feasible that examination of other metal ions in the hairpin RNA
reaction would provide a similar fit as found for calcineurin.
ACKNOWLEDGMENT
These studies were supported with funds from the University of Tennessee Medical
Group.
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