SOLVENT EFFECTS IN 5-DEAZAFLAVIN OXIDATION AS A MODEL FOR NAD+ DEPENDENT ENZYMES

Article abstract of DOI:10.1016/S0040-4039(00)87104-X

The oxidation rate of 3,5-di-t-butylsalicyl alcohol by 3-methyl-10-ethyl-5-deazaisoalloxazine was found to be very sensitive to solvent effect, the rate constant in 99 volpercent DMF being greater by a factor of 37 than that in 50:50 (v/v) DMF-water mixed solvent.The result suggests the importance of the aprotic nature of the reaction environment during the oxidation of alcohols by NAD⁺.