Site-specific labelling of caged ATP with deuterium or 18oxygen

Article abstract of DOI:10.1002/jlcr.2580360312

[3-D]2-Nitroacetophenone and [alcohol-¹⁸O]-1-(2-nitrophenyl)ethyl alcohol were prepared and used to synthesise labelled P³-1-(2-nitrophenyl)ethyl esters of ATP ('caged ATP') with isotope present either as deuterium on the 3-position of the nitrosubstituted ring or as ¹⁸oxygen in the bridging position between the terminal phosphate and the nitrophenylethyl group. The availability of the deuterated compounds enabled complete assignment of their ¹H NMR spectra.