Preparation of [11C]-thymidine and [11C]-2'-arabino-2'-fluoro-β-5-methyl-uridine (FMAU)

Article abstract of DOI:10.1002/jlcr.2580361204

A series of hollow fiber membranes containing immobilized enzymes were prepared and used in the synthesis of ¹¹C-labelled nucleosides. ¹¹C-Formaldehyde was produced in an alcohol oxidase/catalase bioreactor and circulated through a thymidylate synthase bioreactor with an appropriate substrate to produce the corresponding ¹¹C-nucleotide. These labelled nucleotides were subsequently dephosphorylated in an alkaIine phosphatase bioreactor. The bioreactor approach was amenable to hot-cell conditions and yielded ¹¹C-products in higher yield and shorter synthesis times than conventional chemical approaches.