Medium-chain fatty acids from Eugenia winzerlingii leaves causing insect settling deterrent, nematicidal, and phytotoxic effects

Article abstract of DOI:10.3390/molecules24091724

Eugenia winzerlingii (Myrtaceae) is an endemic plant from the Yucatan peninsula. Its organic extracts and fractions from leaves have been tested on two phloem-feeding insects, Bemisia tabaci and Myzus persicae, on two plant parasitic nematodes, Meloidogyne incognita and Meloidogyne javanica, and phytotoxicity on Lolium perenne and Solanum lycopersicum. Results showed that both the hexane extract and the ethyl acetate extract, as well as the fractions, have strong antifeedant and nematicidal effects. Gas chromatography-mass spectrometry analyses of methylated active fractions revealed the presence of a mixture of fatty acids. Authentic standards of detected fatty acids and methyl and ethyl derivatives were tested on target organisms. The most active compounds were decanoic, undecanoic, and dodecanoic acids. Methyl and ethyl ester derivatives had lower effects in comparison with free fatty acids. Dose-response experiments showed that undecanoic acid was the most potent compound with EC₅₀ values of 21 and 6 nmol/cm² for M. persicae and B. tabaci, respectively, and 192 and 64 nmol for M. incognita and M. javanica, respectively. In a phytotoxicity assay, medium-chain fatty acids caused a decrease of 38-52% in root length and 50-60% in leaf length of L. perenne, but no effects were observed on S. lycopersicum. This study highlights the importance of the genus Eugenia as a source of bioactive metabolites for plant pest management.

Full text of DOI:10.3390/molecules24091724

molecules
Article
Medium-Chain Fatty Acids from Eugenia winzerlingii
Leaves Causing Insect Settling Deterrent,
Nematicidal, and Phytotoxic Effects
Angel Cruz-Estrada 1,2, Esaú Ruiz-Sánchez 2, Jairo Cristóbal-alejo 2,
Azucena González-Coloma 3 , María Fe Andrés 3 and Marcela Gamboa-Angulo 1,
*
1
Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, Colonia Chuburná de Hidalgo,
Mérida C.P. 97200, Yucatán, Mexico; angel_estrad@yahoo.com.mx
Tecnológico Nacional de México, Instituto Tecnológico de Conkal, Conkal C.P. 97345, Yucatán, Mexico;
esau.ruiz@itconkal.edu.mx (E.R.-S.); jairoca54@hotmail.com (J.C.-a.)
Instituto de Ciencias Agrarias-CSIC, 115 Dpdo-28006 Madrid, Spain; azu@ica.csic.es (A.G.-C.);
mafay@ica.csic.es (M.F.A.)
2
3
*
Correspondence: mmarcela@cicy.mx; Tel.: +52-999-942-8330
ꢀꢁꢂꢀꢃꢄꢅꢆꢇ
ꢀꢁꢂꢃꢄꢅꢆ
Academic Editor: Vincenzo De Feo
Received: 24 March 2019; Accepted: 20 April 2019; Published: 3 May 2019
Abstract: Eugenia winzerlingii (Myrtaceae) is an endemic plant from the Yucatan peninsula. Its organic
extracts and fractions from leaves have been tested on two phloem-feeding insects, Bemisia tabaci
and Myzus persicae, on two plant parasitic nematodes, Meloidogyne incognita and Meloidogyne javanica,
and phytotoxicity on Lolium perenne and Solanum lycopersicum. Results showed that both the hexane
extract and the ethyl acetate extract, as well as the fractions, have strong antifeedant and nematicidal
effects. Gas chromatography-mass spectrometry analyses of methylated active fractions revealed the
presence of a mixture of fatty acids. Authentic standards of detected fatty acids and methyl and ethyl
derivatives were tested on target organisms. The most active compounds were decanoic, undecanoic,
and dodecanoic acids. Methyl and ethyl ester derivatives had lower effects in comparison with free
fatty acids. Dose-response experiments showed that undecanoic acid was the most potent compound
with EC50 values of 21 and 6 nmol/cm2 for M. persicae and B. tabaci, respectively, and 192 and 64 nmol
for M. incognita and M. javanica, respectively. In a phytotoxicity assay, medium-chain fatty acids
caused a decrease of 38–52% in root length and 50–60% in leaf length of L. perenne, but no effects were
observed on S. lycopersicum. This study highlights the importance of the genus Eugenia as a source of
bioactive metabolites for plant pest management.
Keywords: Bemisia tabaci; decanoic acid; dodecanoic acid; Eugenia winzerlingii; Lolium perenne;
Meloidogyne; Myzus persicae; Solanum lycopersicum; undecanoic acid
1. Introduction
Plant pests are a severe constraint in crop production worldwide. Various species of insects and
nematodes have primary importance due to the enormous losses they cause in crop productivity [1,2].
The sap-feeding and virus-transmitting whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae), and aphid,
Myzus persicae (Hemiptera: Aphididae), are two of the most damaging phytophagous insects in
vegetable crops [3,4]. Likewise, the plant parasitic nematodes Meloidogyne incognita and Meloydogine
javanica are among the most important species of root-knot nematodes in horticultural and field crops,
while the population establishment of B. tabaci and M. persicae directly affects leaf-related physiological
processes and the colonization of Meloidogyne spp. impairs the function of plant roots, reducing
nutrient and water uptake, resulting in poor plant growth and a decline in fruit yield [5].
Molecules 2019, 24, 1724; doi:10.3390/molecules24091724
www.mdpi.com/journal/molecules

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Crop protection against parasitic nematodes and phytophagous insects has been historically
addressed through the use of synthetic pesticides. However, pesticide resistance and environmental
pollution have led to the search for biorational strategies to manage plant pests [
products are considered to be environmentally friendly and represent cost-effective alternatives to
control phytophagous insects and plant pathogenic microorganisms [ 11]. Moreover, an extensive
number of studies have reported the potential of a wide range of plant-derived metabolites against
insects and nematodes [12 14]. The screening of new biologically active compounds from new sources
6–8]. Plant-derived
9–
–
is a key step in the development of more active compounds [15].
The genus Eugenia is one of the largest members of Myrtaceae family distributed in tropical and
subtropical regions from Mexico to Central and South America, with estimations of 1000 species [16,17].
In Mexico, 81 species have been documented from the genus Eugenia [18]. Previous studies by our group
have found that the organic extracts from Eugenia winzerlingii Standl. leaves have strong oviposition
deterrent effects on the whitefly Bemisia tabaci (Homoptera: Aleyrodidae) and have a lethal effect on
the root-knot nematode Meloidogyne incognita [19,20]. The species E. winzerlingii (Figure 1) is a shrub
that grows in the Yucatan peninsula, Belize, and Guatemala [21]. To our knowledge, no purification or
identification of the active constituents of E. winzerlingii have been carried out thus far.
Figure 1. (A) Eugenia winzerlingii Standl., shrub in field, (B) flowers, and (C) fruits.
Secondary metabolites of leaves, barks, seeds, and fruits from approximately 350 Eugenia species,
have diverse biological activity, including analgesic, antioxidant, antifungal, antimicrobial, antidiabetic,
anti-inflammatory, and cytotoxic [16
of Eugenia spp. and their constituents are active against bacteria, fungi, and protozoan [16
However, studies focusing on the effects of extracts or metabolites on plant pests are scarce [27
,22]. Various studies have reported that essential oils of the leaves
,22
–
26].
28].
,
The toxicity of essential oils and organic extracts of Eugenia caryophyllata leaves has been reported
in the human ectoparasite Pediculus capitis [29], the mosquito Aedes aegypti [30], the stored grain
pests Acanthoscelides obtectus, Sitophilus zeamais, and Tribolium castaneum [31,32] and the plant feeders
Oryctes agamemnon, Pochazia shantungensis, and Trialeurodes vaporariorum [33 35], and in the nematodes
–
Bursaphelenchus xylophilus and Meloidogyne incognita [36]. In addition, essential oils from Eugenia uniflora
have shown strong repellent effects on Diaphania hyalinata [37].
In this paper, in the search for novel plant-derived metabolites from regional plants, we have
identified the active components causing insect deterrent and nematicidal activity of the organic
extracts from E. winzerlingii leaves using a partial bioassay-guided fractionation of the hexane and
ethyl acetate extracts. In the bioassays, four economically important plant pest species were used:
The sap-feeding insects B. tabaci and M. persicae and the root-knot nematodes M. incognita and M.
javanica. In addition, the phytotoxicity of the identified compounds was assessed on Lolium perenne L.
(Apoaceae) and Solanum lycopersicum (Solanaceae).

Molecules 2019, 24, 1724
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2. Results
2.1. Effect of Extracts and their Fractions on Insect Settling Deterrence and Nematode Mortality
The hexane (HEX) and ethyl acetate (ETA) extracts obtained from E. winzerlingii leaves showed
a strong settling inhibition (SI) of M. persicae (SI, 87.9–96.9%) and mortal (M) effect on second stage
juveniles (J2) of M. incognita and M. javanica (M, 100%). Chromatographic separation of the HEX and
ETA extracts yielded six (1a–1f) and four (2a–2d) fractions, respectively. Among the fractions obtained,
1b, 1c, 1d, 2b, and 2c showed the strongest SI effect on M. persicae (>90%); while 1b, 1c, 2b, and 2c
fractions also had the strongest effect on M. incognita and M. javanica (M, 100%) (Table 1).
Table 1. Effect of extracts and fractions from Eugenia winzerlingii leaves on Myzus persicae (100
and on two root-knot nematodes Meloidogyne incognita and Meloidogyne javanica (1 µg/µL).
µ
g/cm2)
% SI
% M
Extracts/Fractions
M. persicae
M. incognita
M. javanica
Hexane extract
87.9 ± 2.1 ab
60.3 ± 5.6 c
97.6 ± 1.4 a
94.4 ± 2.1 a
90.9 ± 2.2 ab
60.4 ± 7.7 c
70.4 ± 6.9 bc
100 ± 0.0 a
25.0 ± 9.2 c
100 ± 0 a
100 ± 0 a
32.9 ± 7.8 b
0 ± 0 d
100 ± 0.0 a
5.53 ± 0.92 d
100 ± 0.0 a
1a
1b
1c
1d
1e
1f
100. ± 0.0 a
3.92 ± 1.13 d
4.13 ± 0.52 d
3.92 ± 0.46 d
0 ± 0 d
Ethyl acetate extract
96.9 ± 1.2 a
100 ± 0 a
20.7 ± 13.6 b
100 ± 0 a
100 ± 0 a
0 ± 0 c
100 ± 0.0 a
11.39 ± 3.3 b
100.0 ± 0.0 a
100.0 ± 0.0 a
13.47 ± 4.8 b
2a
2b
2c
nt
97.6 ± 1.1 a
91.3 ± 2.7 b
89.7 ± 2.1 b
2d
Mean of settling inhibition
the same column are not significantly different (Tukey, p < 0.05); nt = not tested.
±
standard error (% SI
±
SE) and mortality (% M
±
SE) followed by the same letter within
2.2. Major Constituents of Active Fractions
The most active fractions 1b and 1c obtained from the HEX extract and 2b and 2c fractions
from ETA extract were analyzed by gas chromatography–mass spectrometry (GC-MS). All fractions
contained a mixture of saturated fatty acids identified as decanoic, undecanoic, dodecanoic, tridecanoic,
and tetradecanoic acids. In order to verify the presence of these and other fatty acids in the fractions,
their methyl esterifed derivatives were obtained. The most abundant component was dodecanoic acid
(32.41–43.56%) in all active fractions. The fraction 1c did not contain the first two medium-chain fatty
acids. The long-chain fatty acid, hexadecanoic acid, was present in all fractions except 2c (Table 2;
Figures S1 and S2).

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Table 2. Chemical composition found in the methylated fractions of organic extracts from Eugenia
winzerlingii leaves by gas chromatography–mass spectrometry (GC-MS) analysis.
Fractions (%)
Compound
Retention Time (min)
1b
1c
2b
2c
Methyl decanoate
Methyl undecanoate
Methyl dodecanoate
Methyl tridecanoate
Methyl tetradecanoate
Methyl hexadecanoate
4,8,12,16-Tetramethylheptadecan-4-olide
Unknown
7.27
8.59
9.99
11.25
12.41
14.52
18.24
22.04
22.21
27.61
4.75
17.24
40.22
10.42
9.07
6.55
17.46
43.56
13.67
12.77
2.46
7.08
17.89
37.81
10.58
7.51
32.41
12.29
12.51
4.08
8.7
17.02
5.90
2.18
Unknown
Unknown
16.1
7.50
Compounds were identified by comparison to the NIST05 chemical database and commercial standards.
Only compounds with a probability of matching mass spectra > 0.9 are shown.
2.3. Biological Activity of Fatty Acids and Derivatives
All saturated fatty acids and their methyl/ethyl ester derivatives were evaluated on insect SI,
oviposition inhibition (OI), and nematode mortality. Overall, methyl ester derivatives had similar or
slightly lower effects in comparison with free fatty acids, whereas ethyl derivatives had significantly
lower effects, particularly on M. javanica (Table 3). Decanoic, undecanoic, and dodecanoic acids had
the greatest effect on insect SI. Values for SI of M. persicae were 83.3–97.6% and for OI of B. tabaci they
were 94.3–98.2%. All fatty acids also showed strong lethal effects on M. incognita and M. javanica J2
(M = 94.4–100%) (Table 3).
Table 3. Effect of fatty acids and ester derivatives on deterrence of two sap-sucking insects, Myzus
persicae and Bemisia tabaci (50 µ
g/cm2), and mortality of two root-knot nematodes, Meloidogyne incognita
and Meloidogyne javanica (0.5 µg/µL).
% SI
Compound
% OI
% M
M. persicae
B. tabaci
M. incognita
M. javanica
Decanoic acid
Undecanoic acid
Dodecanoic acid
89.7 ± 2.5 ab
83.3 ± 3.4 ab
97.6 ± 1.4 a
98.2 ± 1.7 a
94.3 ± 3.3 a
94.7 ± 2.9 a
Nt
100 ± 0 a
100 ± 0 a
100 ± 0 a
94.4 ± 6.4 a
100 ± 0 a
100 ± 0 a
nt
100.0 ± 0.0 a
100.0 ± 0.0 a
94.40 ± 0.4 b
nt
90.86 ± 2.5 b
98.7 ± 0.5 a
95.4 ± 1.0 b
14.53 ± 0.61 c
nt
Tridecanoic acid
53.8 ± 8.9 c
Tetradecanoic acid
Methyl decanoate
Methyl undecanoate
Methyl dodecanoate
Methy tridecanoate
Methyl tetradecanoate
Ethyl decanoate
Ethyl undecanoate
Ethy dodecanoate
Ethyl tetradecanoate
76.2 ± 4.7 b
59.1 ± 9.2 b
10.1 ± 6.2 de
15.7 ± 14.2 cde
0.0 ± 0 e
36.3 ± 8.8 fg
33.8 ± 7.1 fg
48.8 ± 7.1 efg
57.7 ± 8.2 cdef
93.3 ± 0.2 ab
34.4 ± 6.9 fg
37.4 ± 34.1 fg
52.7 ± 7.6 defg
77.4 ± 4.3 b
100 ± 0 a
74.8 ± 5.8 b
100 ± 0 a
55.9 ± 11.7 b
53.9 ± 2.1 b
61.6 ± 3.9 b
63.8 ± 3.6 b
Nt
16.4 ± 10.2 bcde
30.7 ± 16.2 bcde
46.6 ± 11.9 bcd
62.9 ± 12 ab
42.2 ± 13.6 bcd
10.24 ± 1.9 cd
5.91 ± 0.3 de
16.71 ± 3.2 c
3.28 ± 0.2 e
3.64 ± 0.4 e
Mean of settling inhibition (% SI
same letter within the same column are not significantly different. One-way ANOVA followed by Tukey
±
SE), oviposition inhibition (% OI
±
SE), and mortality (% M
±
SE) followed by the
s multiple
´
comparisons test (p < 0.05); nt = not tested.
Dose-response experiments to calculate the median effective concentrations (EC50) indicated that
undecanoic acid was the most potent compound on all target organisms (Table 4, Figure 2).

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Table 4. Median effective concentrations (EC50) of fatty acids on settling deterrence of two sap-sucking
insect species, Myzus persicae and Bemisia tabaci, and median lethal concentration (LC50) of two root-knot
nematodes, Meloidogyne incognita and Meloidogyne javanica.
EC50 (nmol/cm2)
LC50 (nmol/mL)
Compound
M. persicae
B. tabaci
M. incognita
M. javanica
Decanoic acid
Undecanoic acid
Dodecanoic acid
*
95 (91–99) a
6 (2–11) c
49 (42–55) b
229 (218–240) a
192 (186–199) b
231 (220–241) a
85 (83–87) b
64 (62–66) c
368 (323–410) a
21 (11–30) a
*
* Data reported by Santana et al. [38]. EC50 and LC50 values followed by the same letter within the same column are
not significantly different.
Figure 2. The most active medium-chain fatty acids from Eugenia winzerlingii leaves, (
A) decanoic acid,
(B) undecanoic acid, and (C) dodecanoic acid.
2.4. Phytotoxicity of Fatty Acids
The evaluation of phytotoxicity of the most active medium-chain fatty acids on two plant species
showed that none of the compounds affected germination or rootlet growth of the dicotyledonous
Solanum lycopersicum. Similarly, HEX extract had no effect on these variables. In contrast, ETA extract
caused a slight effect on the rootlet length of S. lycopersicum. All fatty acids caused a significant decrease
in growth of rootlet and leaves of the monocotyledonous Lolium perenne. In addition, germination was
also affected by undecanoic acid (Table 5).
Table 5. Phytotoxic effects of fatty acids (50
Solanum lycopersicum.
µ
g/cm2) and extracts (100
µ
g/cm2) on Lolium perenne and
Extract
Lolium perenne a
Solanum lycopersicum a
Fatty Acid
Germination
Root Length
Leaf Length
Germination Rootlet Length
Hexane extract
Ethyl acetate extract
Decanoic acid
Undecanoic acid
Dodecanoic acid
a
62.16 ± 9.62
72.97 ± 11.47
72.2 ± 10.1
58.3 ± 7.5 *
94.4 ± 4.5
61.21 ± 8.05
76.79 ± 8.61
62.3 ± 11.0 *
48.1 ± 11.0 *
58.7 ± 8.7 *
81.36 ± 10.30
80.83 ± 8.22
50.5 ± 10.4 *
39.2 ± 13.4 *
46.4 ± 9.2 *
102.56 ± 0.0
97.44 ± 7.52
125.8 ± 4.6
129.0 ± 0.0
122.6 ± 9.3
94.49 ± 8.64
67.96 ± 5.46
88.1 ± 12.0
87.1 ± 11.7
118.3 ± 13.2
Percentage of control. Values are means (
±
SE). * indicate significant differences of their own control (p < 0.05,
Mann Whitney test).
3. Discussion
In the search for novel plant-derived compounds for pest management, we have found that the
organic extracts from E. winzerlingii leaves were promissory as insect antifeedant and nematicidal
agents [19,20]. In the present work, we have shown that the ETA and HEX extracts from E. winzerlingii
leaves, as well as fractions 1b, 1c, 2b, and 2c showed strong anti-settling effects on the aphid M. persicae
and nematicidal effects on M. incognita and M. javanica (Table 1). In a previous work, we documented
that these extracts also caused a high mortality rate in nymphs (LC50 of 0.25–0.78 mg/mL) of B.
tabaci, and ETA extract had good oviposition inhibition effects on adults (EC50 of 27.86
Furthermore, among 20 native plant species tested, the total ethanol extract of E. winzerlingii leaves
was the most effective on J2 M. incognita with a median effective doses (ED50) of 133.4 g/mL [20].
In comparison with studies using essential oils of other species of Eugenia, the organic extracts from E.
µ
g/cm2) [19].
µ

Molecules 2019, 24, 1724
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winzerlingii leaves have a similar potency to that of the essential oils from E. uniflora leaves (10 µg/µL),
as observed in oviposition deterrence and larval repellence of the Lepidopteran Dyapahania hyalinata [37].
In contrast, a higher potency of essential oils of E. caryophyllata leaves (0.4
Hemipteran Pochazia shantungensis [34].
µg/µL) was observed in the
The most active fractions 1b, 2b, and 2c contained a mixture of saturated medium-chain (decanoic,
undecanoic, and dodecanoic acids) and long-chain (tridecanoic, tetradecanoic, and hexadecanoic acids)
fatty acids (Table 2). This is the first report of the effect of a Eugenia species on the aphid M. persicae and
the parasitic nematode M. javanica. To our knowledge, isolation and biological activity of medium-chain
fatty acids from the Eugenia genus have not been previously reported. Thus far, phytochemical
studies of other Eugenia species with pesticidal properties have reported the presence of caryophyllene
oxide, cineol, cymene, elemene, eudesmol, eugenol, furanodiene, furanoelemene, germacrene, linalool,
phytol, Selina-1,3,7(11)-trien-8-one, and terpinene, as the most abundant metabolites in essential oils of
leaves [22–27,37,39–41]. It was surprising to find that E. winzerlingii leaves do not biosynthesize essential oils.
Plant-derived medium-chain fatty acids are mainly found in the seeds of oleaginous species [42].
These kinds of metabolites are also ubiquitous in plant cuticle waxes, where they are more likely to play an
important role at the interface between plants and phytophagous insects [43]. Various studies provide
evidence that the exogenous application of fatty acids alters the settling or feeding behavior of insects.
Fatty acids can either deter or attract insects, depending on their structures and lengths. For example,
saturated medium-chain (decanoic, undecanoic, and dodecanoic acids), and long-chain unsaturated fatty
acids (oleic, linoleic, and linolenic acids) strongly inhibited settling by the aphid M. persicae, but long-chain
saturated fatty acids (palmitic and stearic acids) showed the opposite effect [38,44].
In the present study, decanoic, undecanoic, and dodecanoic acids caused oviposition inhibition in
B. tabaci, which had not previously been reported. Undecanoic acid inhibited settling in M. persicae
with the same potency that decanoic and dodecanoic acids (25 and 21 nmol, respectively), as previously
reported [38,45]. In agreement with our results, deterrent effects of medium-chain fatty acids have been
observed in other insect species, such as the mosquitoes Aedes aegypti, Anopheles albimanus, Anopheles
quadrimaculatus, and Culex quinquefasciatus [46–48] and the aphid Rhopalosiphum maidis and the beetle
Leptinotarsa decemlineata [38]. The electrophysiological response of insects to fatty acids causing
anti-feeding or oviposition deterrence effects has been related to the stimulation of species-specific
sensilla in the antennae [48,49]. However, the mechanisms of how these compounds interact with odor
receptors and gustatory receptors have not been studied.
In this work, the fractions that caused insect settling deterrence also showed lethal effects on M.
incognita and M. javanica. Consistent with our results, studies by other authors have demonstrated
the nematicidal activity of fatty acids [11
,50
,51]. In comparison to those studies, we have reported
lower EC50 values for decanoic and dodecanoic acids on M. incognita juveniles [51
,52]. The actions of
fatty acids on nematodes have been associated with the disruption of cell membranes, which leads to
alterations in membrane permeability and ion transport, which in turn compromises internal ion and
water homeostasis [50]. In addition, fatty acids may also alter chemotactic behavior in nematodes,
which results in a substantial decrease in nematode infection [52].
It is worth mentioning that potassium or sodium salts of fatty acids have shown high efficacy not
only on insect deterrence, but also on insect mortality [53,54]. Salts of fatty acids, either alone or in
combination with free fatty acids, may kill insects by suffocation and through changes of cuticle and
cell permeability [54].
Even though fatty acids have shown the potential to manage insects and nematodes in agriculture,
it is important to determine whether the use of these compounds is safe for plants and, therefore,
feasible for use as plant protection agents. In this regard, we observed that decanoic, undecanoic,
and dodecanoic acids may be phytotoxic to some plant species, given that they reduced germination
and root length of L. perenne, but caused no effect on S. lycopersicum. Toxic effects of fatty acids on plant
germination have been documented in various studies [55
,56]. The effects of medium-chain fatty acids
on seed germination have been linked to changes in the physical properties of membranes, which

Molecules 2019, 24, 1724
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alters the kinetics of their associated enzymes, affecting water potential and ionic exchange of the
embryonic axes [57 58]. We suggest that prior to the use of any fatty acid as a plant protection agent,
,
phytotoxicity tests must be carried out on the plant species in question.
In summary, extracts from E. winzerlingii leaves had strong deterrent effects on two phytophagous
insects B. tabaci and M. persicae, as well as nematicidal effects on M. incognita and M. javanica. The active
fractions of the extracts contained a mixture of saturated fatty acids. Evaluation of authentic standards
of the identified compounds showed that decanoic, undecanoic, and dodecanoic acids were the most
active against all species evaluated. This study contributes to the search for biorational agents for
sustainable plant pest management and enriched the phytochemistry knowledge of E. winzerlingii.
Further investigation is needed in order to evaluate the potential for phytotoxicity of these compounds
in relevant plant species.
4. Materials and Methods
4.1. Plant Material and Extraction
Fresh leaves of E. winzerlingii were collected in Calakmul, Campeche, M
360 15.1” SE 49◦ 160 0.7” W). A voucher specimen (PS3021) was deposited at the herbarium “Roger
Orellana Lanza” of the Unidad de Recursos Naturales of Centro de Investigaci n Cient fica de Yucat n.
Leaves were dried under artificial light (50–60 ◦C) for three days and were ground [19].
Ground leaves (4 kg) were extracted with hexane (3 4 L, 24 h, at room temperature). After this
é
xico (coordinates 12◦
ó
í
á
×
process, the residual plant material was subjected to extraction with ethyl acetate, as carried out for
hexane extraction. The solvent from the extractions was eliminated by a low-pressure vacuum at 40 ◦C
in a rotary evaporator (Ika RV 10 Control; Ika Works, Inc., Staufen, Germany). The yield of each extract
relative to the initial dry mass of ground leaves used was 0.4% for the hexane extract and 2.1% for the
ethyl acetate extract.
4.2. Chromatographic Fractionation of Organic Extracts
The hexane extract (14.0 g) was fractionated through a vacuum liquid column (3 cm diameter)
packed with silica gel 60 (Merck, Darmstadt, Germany) and eluted with mixtures of hexane-acetone
(250 mL, each) of increasing polarity, to yield six fractions (1a–1f). The ETA extract (37.7 g) was filtered
with ethyl acetate by clay Tonsil®(Bleaching Earths, Clariant, Moosburg, Germany). The concentrated
filtrate (8.5 g) was fractionated through silica gel packed in a vacuum liquid column (3 cm diameter)
and eluted with mixtures of hexane-ethyl acetate (200 mL each) mixture of increasing polarity to yield
four fractions (2a–2d) [59]. Pre-coated silica gel plates (Kieselgel 60 F254, 0.25 mm; Merck) were used
for thin layer chromatography (TLC). Detection in the TLC was achieved under ultraviolet light by
spraying it with a phosphomolybdic acid reagent, followed by heating for 5 min at 105 ◦C.
4.3. Preparation of Methyl and Ethyl Esters
Prior to Gas Chromatography-Mass Spectrometry (GC-MS) analysis, active fractions and
commercial decanoic acid, undecanoic acid, dodecanoic acid, tridecanoic acid, and tetradecanoic acid
(purity > 98%; Sigma–Aldrich, St. Louis, MI, USA) were methylated and ethylated. Samples (30 mg)
were diluted with 10 mL acidified methanol or ethanol (1% sulfuric acid) and stirred for 12 h at room
temperature. The product of the reaction was suspended in an aqueous solution of 20% Na2CO3
(20 mL) and extracted with CHCl3 (3 , 20 mL, each one) and the solvent was eliminated under vacuum
×
until dry. These derivatives were stored at 4 ◦C until use in assays [60].
4.4. Analytical Method
Analysis of the esterified fractions and esterified commercial standards were performed by GC-MS
in an Agilent 6890N Network Gas Chromatograph coupled to an Agilent 5975B Mass selective detector
(Santa Clara, CA, USA). Samples (0.5 µL, 1% dichloromethane solution) were injected in an Ultra

Molecules 2019, 24, 1724
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1 Agilent GC capillary column (dimethylpolysiloxane, 25 m
×
0.32 mm, 0.52
µ
m film thickness).
Helium was used as a carrier gas at a flow rate of 1 mL/min, and the column temperature was initially
held at 150 ◦C for 10 ◦C/min, then raised to 280 ◦C for 20 min. Major individual components were
identified by comparing their mass spectra with those of the spectrometer database using the NIST
05 library and by comparison with commercial standards.
4.5. Bioassays
All fractions obtained from HEX and ETA extracts were evaluated on the settling inhibition of
M. persicae and mortality of J2 M. incognita and M. javanica. The activity of authentic standards of the
medium-chain fatty acids present in the active fractions and their methylated and ethylated esterified
derivatives, obtained as described above, were evaluated in the same three targets as well as on B. tabaci.
4.5.1. Evaluation of settling inhibition on Myzus persicae
A colony of M. persicae was reared on bell pepper (Capsicum annuum) and maintained in a growth
chamber at 24
±
2 ◦C, 60–70% relative humidity and a photoperiod of 16L:8D. The settling bioassay was
conducted in a choice experiment, as described by Castillo [44]. Leaf disks (2 cm2) were cut from a fully
expanded C. annuum leaf, divided into two half-disks, and placed on the bottom of a plastic box (3 cm
×
3 cm
and fractions (10
×
1.5 cm) with a ventilated lid and a bottom lining of 2% agar. An aliquot (10
µL) of extracts
µ
g/ L), or authentic fatty acid standards (5 g/ L) dissolved in acetone:water (8:2),
µ
µ µ
were deposited on the surface of one of the half-disks. For control, a second half-disk received only the
solvent mixture. After solvent evaporation, 10 newly emerged adults of M. persicae were transferred to
each box. Twenty replicates were used for each treatment. The boxes were incubated at 22 ◦C and a
photoperiod of 18L:6D. The number of aphids settled on the treated and control leaf sections were
recorded after 24 h. The percentage of settling inhibition (SI) was calculated by the formula:
SI = [1 − (%T/%C)] × 100
(1)
where T and C are the number of insects settled on the treated and control leaf sections, respectively [57].
For serial dilutions, extracts or fractions were evaluated at 100, 50, and 25 µ
g/cm2, while authentic
standards were evaluated at 50, 25, and 12.5 µg/cm2.
4.5.2. Evaluation of oviposition inhibition on Bemisia tabaci
Deterrence effects on B. tabaci oviposition was only evaluated with authentic standards of
medium-chain fatty acids. The effects of extracts have been reported elsewhere [19].
A whitefly colony was reared on habanero pepper (Capsicum chinense) within entomological
◦
cages (1.44 m3) in a greenhouse at 25–35 C, 55–75% relative humidity, and a photoperiod of
12L:12D. The colony was maintained for several years and has been used in previous studies [61].
Oviposition deterrence was evaluated as described by Baldin et al. [62], with some modifications.
Leaf sections (2 cm2) were cut and treated at the same concentrations as described for M. persicae.
Single treated and control leaf sections were set on the bottom of a Petri dish (3 cm diameter).
After solvent evaporation, 16 unsexed adults (3–5 days after emergence) were transferred to each Petri
dish and incubated at 26
±
2 ◦C, 75
±
8% relative humidity and a photoperiod of 16L:8D. Five replicates
(Petri dishes) were used for each treatment. The number of eggs laid on each leaf section was recorded
24 h after adult transfer. The percentage of oviposition inhibition (OI) was calculated using the equation:
OI = [1 − (T/C)] × 100
(2)
where T and C are the number of eggs on the treated and control leaf sections, respectively.

Molecules 2019, 24, 1724
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4.5.3. Nematicidal Assay
The colonies of M. incognita and M. javanica were maintained on tomato plants (var. Marmande)
and cultivated in plastic pots in a growth chamber at 25
±
1 ◦C and 70% relative humidity. For bioassays,
egg masses from infected tomato roots were incubated in distilled water at 25 ◦C for 24 h to obtain
second-stage juveniles (J2). Nematicidal activity was evaluated in 96-microwell plates as previously
described by Andr
(5 L) of extracts or fractions (20
Subsequently, 95 l of distilled water containing 100–150 juveniles were added in each well, with a
final concentration of 1 g/ L and 0.5 g/ L, respectively. Control wells received a 5 L of solvent
és [36] with some modifications. Experimental microwells received a sample
µ
µ
g/ L), or fatty acids (10 g/ L) dissolved in DMSO:0.5%-Tween 20.
µ
µ µ
µ
µ
µ
µ
µ
µ
mixture. All treatments were replicated four times. Plates were covered to prevent evaporation and
kept in darkness at 25 ◦C, for 72 h. Dose-response experiments were conducted using concentrations
that ranged from 1 to 0.0312 µg/µL.
Mortality of juveniles was recorded with the aid of a stereoscopic microscope at 40 after 72 h.
×
The percentages of juvenile mortality in the microwell assays were corrected by elimination of the
natural death/mortality of the control:
M = [(% mortality in experimental microwells − % mortality in control microwells)/
(3)
(100 − % mortality in control microwells) × 100].
4.5.4. Phytotoxic Assay
Phytotoxicity of HEX, ETA, and active fatty acids were tested on L. perenne L. and
S. lycorpersicum [63]. Samples (20
solutions were added to 2.5 diameter filter paper (Whatman grade 2) and placed on 12 well plates.
After solvent evaporation, groups of 10 seeds and 500 L H2O were added to each well. Control filter
paper received only the solvent. Juglone (5 g/ L) was included as a positive control. Plates were
incubated in a growth chamber (24
2 ◦C, 16:8 L:D). Seed germination was measured daily for seven
µL) of fatty acids (5 µg/µL) and organic extracts (10 µg/µL)
µ
µ
µ
±
days for L. perenne and five days for S. lycopersicum. Germination was considered successful when
radical emerge by rupturing the seed coat. The length of rootlet and leaf were measured by the
digitalization of pictures (ImageJ v1.43; http://rsb.info.nih.gov./ij/) at the end of the experiment in
20–26 plantlets randomly selected for each treatment.
4.6. Data Analysis
Data on percentage M, OI, SI were analyzed by one-way ANOVA. Prior to running the data,
these were arc-sin transformed in order to satisfy the ANOVA assumption of homogeneity of variance.
Mean separation was performed by the Tukey post hoc test,
α 0.05. The EC50 values were calculated
by the Probit analysis. Phytotoxic effects were analyzed by the Mann–Whitney test. All analyses were
conducted in the Statistical Analysis System (SAS) software (SAS Institute, Cary, NC, USA), version
8.1 for Windows. Mean differences were considered significant if p < 0.05.
5. Patents
A part of this work resulted in a patent, which is being evaluated by the Instituto Mexicano de
protection intellectual.
Supplementary Materials: The following are available online at http://www.mdpi.com/1420-3049/24/9/1724/s1,
Figure S1: Gas chromatogram of fractions obtained from hexane extract of Eugenia winzelingii leaves, Figure S2:
Gas chromatogram of fractions obtained from ethyl acetate extract of Eugenia winzelingii leaves.
Author Contributions: Conceived the project, M.G.-A.; E.R.-S.; A.C.-E.; performed the experiments, collected and
analyzed data, A.C.-E.; supervised and provided biological material for assay on B. tabaci, E.R.-S.), M. persicae,
A.G.-C., M. javanica, M.F.A., and M. incognita, J.C.-a.; supervised the chromatographic fractionation, derivatization
reactions, and GC-MS analyses, M.G.-A.; writing—reviewed manuscript by all authors. Each author approved the
final version of the manuscript of submission.

Molecules 2019, 24, 1724
10 of 13
Funding: This work was supported by projects PDCPN-2015-266, FOMIX- Yuc-2011-C09-168624 (CONACYT),
and grant CTQ2015-64049-C3-1-R (MINECO/FEDER). Angel Cruz-Estrada thanks Conacyt for the Ph.D.
scholarship (234765).
Acknowledgments: Authors are grateful to I.L. Medina-Baizabal, F. Moo-Koh, A. P. Simá-Polanco, and E. Balam-Uc
for their technical assistance.
Conflicts of Interest: The authors declare no conflict of interest.
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Sample Availability: Samples of the compounds are not available from the authors.
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