Effect of Dimethylsulfoxide on Hydrolysis of Lipase

Article abstract of DOI:10.1271/bbb.65.2078

To establish an industrially feasible reaction process, the effect of dimethylsulfoxide (DMSO) added to an aqueous solution on the hydrolysis of lipase was investigated using fluorescent substrates. Several lipases from microorganisms were improved in their hydrolysis activities against 4-methylumbelliferyl oleate by DMSO. Variation was found in the effect of DMSO depending on the species of lipase. After the high stability of the lipase from Pseudomonas fluorescens in DMSO solution was confirmed, hydrolysis by this lipase of four acyl-4-methylumbelliferones was studied kinetically at different DMSO concentrations. DMSO added to an aqueous solution increased the V_max of this lipase for a substrate with strong hydrophobicity, and decreased that value for a substrate with an opposite property. On the other hand, DMSO had a very small effect on K_m for each substrate. A fluorometric study suggested that DMSO induced a change of the chemical environment that surrounded tryptophan residues of the lipase. Such conformational change would be one of the causes of the DMSO-induced alteration of its reactive property. These results suggest that the addition of DMSO may be a novel method of 'solvent engineering' of this enzyme.

Full text of DOI:10.1271/bbb.65.2078

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Bioscience, Biotechnology, and Biochemistry
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Effect of Dimethylsulfoxide on Hydrolysis of Lipase
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Wakako TSUZUKI , Akemi UE & Yoshiaki KITAMURA
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National Food Research Institute
Published online: 22 May 2014.
To cite this article: Wakako TSUZUKI, Akemi UE & Yoshiaki KITAMURA (2001) Effect of Dimethylsulfoxide on Hydrolysis of
Lipase, Bioscience, Biotechnology, and Biochemistry, 65:9, 2078-2082, DOI: 10.1271/bbb.65.2078
To link to this article: http://dx.doi.org/10.1271/bbb.65.2078
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