Nucleosides, nucleotides and nucleic acids p. 1635 - 1637 (2003)
Update date:2022-08-11
Topics:
Ng, Pei-Sze
Pingle, Maneesh R.
Balasundarum, Ganesan
Friedman, Alan
Zu, Xiaolin
Bergstrom, Donald E.
The syntheses of endcaps for covalently linking the 3′ and 5′ hydroxyl groups of blunt end double-stranded DNA are described. Endcap diols were converted into DMTr protected phosphoramidites and incorporated between nucleotides 4 and 5 of a self-complementary octamer. The stabilizing effect of the endcaps on duplex DNA was determined by Tm experiments on the self-complementary octamer.
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