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New Journal of Chemistry
Page 6 of 8
DOI: 10.1039/C7NJ01407D
ARTICLE
Journal Name
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1.45 (GPC, polystyrene calibration). H NMR (600 MHz, CDCl3), Education of China (20136203120002), the Ministry of
δ (TMS, ppm): 9.85 (CHO), 7.75 and 7.05-7.49 (ArH), 7.71 Education Scholars Innovation Team (IRT 1177) for financial
(CH=C), 1.0-2.6 (CH2). 13C NMR (150 MHz, CDCl3), δ (TMS, ppm): support.
212.19, 190.70, 152.76, 145.83, 131.88, 131.27, 129.88, 129.60,
125.56, 124.57, 41.95, 26.99, 24.97. IR (KBr), v (cm−1): 3062,
References
3036, 2924, 2854, 1690, 1602, 1589, 1504, 1272, 832, 752, 695.
The same process could be applied for the synthesis of BP. BP
was afforded as red powders. Mw = 15820; Mw/Mn = 1.43. 1H
1
P. Kulkarni, M. K. Haldar, S. You, Y. Choi and S. Mallik,
2016,
Biomacromolecules,
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DOI:
NMR (600 MHz, d6-DMSO),
δ (TMS, ppm): 9.92 (CHO), 7.89
2
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and 7.26 (ArH), 7.79 (CH=C), 2.24, 1.8-1.7 and 1.68-1.57 (CH2).
13C NMR (150 MHz, d6-DMSO), δ (TMS, ppm): 210.85, 191.93,
151.25, 132.66, 131.89, 124.89, 41.77, 26.86, 24.77. IR (KBr), v
(cm−1): 3064, 3032, 2923, 2852, 1691, 1601, 1500, 1273, 830,
751, 697.
3
4
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3.3 Cell culture
The cytotoxicity test was performed with MPC5 cells. LP was
sterilized with ultraviolet light and dissolved in DMSO. Then
the solution was diluted with PBS buffer (pH = 7.4) to different
concentrations (30, 40, 50, 60, 70 and 80 μg/mL). To obtain
complete cell culture medium, 10% FBS, 100 units mL−1
penicillin, and 100 units mL−1 streptomycin were added in the
mixture. MPC5 cells (purchased from Gansu Provincial Cancer
Hospital, Gansu, China) were cultured in the conditioned
Hamburger
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5% CO2. After 96 h of incubation, cell viability was determined
bythe3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide (MTT) method.
3.4 Cell imaging
Living MPC5 cells (1.0 × 105 per well) were cultured overnight
and then stained in a Petri dish at 50% confluence with a LP
solution (50 μg/mL). An image was taken after 0.5 h incubation.
4. Conclusions
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In this paper, we present the facile synthetic method by Aldol
Addition and Condensation (AAC), leading to the formation of
florescent linear (LP) and branched polymers (BP) by cross
coupling triphenylamines (TPA) and cyclohexanones (CYC) via
C=C bond. That methodology takes advantages of easy
operations, mild reaction conditions (Inert gas protect-free,
Solvent dryness-free, Moderate temperature). The further
optical analysis reveals that LP and/or BP demonstrate solvent-
dependent chromic effect, AIEE property, organogel formation
ability, thermosensitivity, and better bio-compatibility.
Notably, LP is readily internalized by MPC5 cells, and it
behaves good biocompatibility and high resolution. Therefore,
the readily available materials are of the potential versatile
applications in the fields of bioimaging. Further research is
underway in our lab.
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Acknowledgements
This work is supported by the National Natural Science
Foundation of China (nos. 21202133, 51363019). We thank
Specialized Research Fund for the Doctoral Program of Higher
6 | J. Name., 2012, 00, 1-3
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