Enhancement of the enantioselectivity of penicillin G acylase from E. coli by 'substrate tuning'

Article abstract of DOI:10.1016/0040-4039(95)00440-N

The efficiency of penicillin G acylase catalyzed transformations is enhanced significantly with respect to reaction velocity, and in particular, stereoselectivity by appropriate 'substrate tuning', i.e. by the introduction of a nitrogen atom into the part of the substrates which is recognized by the enzyme.