Interaction of the recombinant Herpes Simplex Virus type 1 thymidine kinase with thymidine and aciclovir: A kinetic study

Article abstract of DOI:10.1080/15257779908043078

Herpes Simplex Virus type 1 thymidine kinase (HSV 1 TK) is a key target for antiviral therapy and it phosphorylates a broad spectrum of nucleosides and nucleotides. We report the results from kinetic and inhibition experiments with HSV 1 TK, and show that there is a preferred, but not exclusive, binding order of substrates, i.e. dT binds prior to ATP. Furthermore, the results provide new informations on the mechanism of binding suggesting that HSV1 TK undergoes conformational changes during the catalytic cycle.

Full text of DOI:10.1080/15257779908043078

Nucleosides and Nucleotides
ISSN: 0732-8311 (Print) (Online) Journal homepage: http://www.tandfonline.com/loi/lncn19
Interaction of the Recombinant Herpes Simplex
Virus Type 1 Thymidine Kinase with Thymidine and
Aciclovir: A Kinetic Study
Susanna Kussmann-gerber , Christine Wurth , Leonardo Scapozza , Beatrice
D. Pilger , Vladimir Pliška & Gerd Folkers
To cite this article: Susanna Kussmann-gerber , Christine Wurth , Leonardo Scapozza , Beatrice
D. Pilger , Vladimir Pliška & Gerd Folkers (1999) Interaction of the Recombinant Herpes Simplex
Virus Type 1 Thymidine Kinase with Thymidine and Aciclovir: A Kinetic Study, Nucleosides and
Nucleotides, 18:3, 311-330, DOI: 10.1080/15257779908043078
To link to this article: http://dx.doi.org/10.1080/15257779908043078
Published online: 04 Oct 2006.
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Date: 29 September 2015, At: 07:23