Angewandte Chemie - International Edition p. 2356 - 2360 (2016)
Update date:2022-08-10
Topics:
?ácha, Pavel
Knedlík, Tomá?
Schimer, Ji?í
Tykvart, Jan
Parolek, Jan
Navrátil, Václav
Dvo?áková, Petra
Sedlák, Franti?ek
Ulbrich, Karel
Strohalm, Ji?í
Majer, Pavel
?ubr, Vladimír
Konvalinka, Jan
Antibodies are indispensable tools for biomedicine and anticancer therapy. Nevertheless, their use is compromised by high production costs, limited stability, and difficulty of chemical modification. The design and preparation of synthetic polymer conjugates capable of replacing antibodies in biomedical applications such as ELISA, flow cytometry, immunocytochemistry, and immunoprecipitation is reported. The conjugates, named "iBodies", consist of an HPMA copolymer decorated with low-molecular-weight compounds that function as targeting ligands, affinity anchors, and imaging probes. We prepared specific conjugates targeting several proteins with known ligands and used these iBodies for enzyme inhibition, protein isolation, immobilization, quantification, and live-cell imaging. Our data indicate that this highly modular and versatile polymer system can be used to produce inexpensive and stable antibody substitutes directed toward virtually any protein of interest with a known ligand.
View MoreContact:+86-0760-85282375
Address:zhongjing road,zhongshan torch hi-tech industrial development zone
Shanghai Haoyuan Chemexpress Co., Ltd.
Contact:86-21-51870955;58998985;58999585
Address:Room 601, No. 2 BLD, NO. 720, Cailun Road, Zhangjiang, Shanghai, China
A.M FOOD CHEMICAL CO., LIMITED
Contact:86-531-87100375
Address:20Floor,Bblock,1Building,pharma-valley,Jinan,China
Beijing Greenchem Technology Co.,Ltd. ( Panjin Greenchem Technology Co.Ltd .)
website:http://www.bjgreenchem.com/
Contact:+86-427-6515887
Address:301,302, 3rd Floor, Building C-7, Dongsheng Science Park, Northern Territory, Zhongguancun, No. 66, Xixiaokou Road, Haidian District, Beijing
puyang hongda shengdao new material co.,ltd.
Contact:+86-393- 4896278
Address:No.29 East Zhongyuan Road
Doi:10.1002/(SICI)1526-4998(200006)56:6<560::AID-PS171>3.0.CO;2-X
(2000)Doi:10.1134/S0036023608060041
(2008)Doi:10.1016/S0022-328X(00)81436-9
(1977)Doi:10.1039/C8NJ05152F
(2019)Doi:10.1016/S0040-4039(01)80367-1
(1989)Doi:10.1002/chir.22120
(2013)