1
34
J.-P. Nam et al. / International Journal of Pharmaceutics 457 (2013) 124–135
Fig. 11. Receptor mediated dependent cellular uptake of rhodamine-labeled OCFP and TPOCFP micelles and measured by flow cytometry. Flow cytometric logarithmic
histogram of cells incubated with OCFP and TPOCFP micelles (A) and various inhibitor such as indomethacin (10 M), chlorpromazine (10 M), sodium azide (100 M), and
colchicine (15 M) incubated before treated OCFP or TPOCFP micelles into the cells (B).
studies are in progress to determine the in vivo efficacy of TPOCFP
micelles as a clinical therapy.
Khalil, I.A., kogure, K., Akita, H., Harashima, H., 2006. Uptake pathways and sub-
sequent intracellular trafficking in nonviral gene delivery. Pharmacol. Rev. 58,
32–45.
Kim, S.C., Kim, D.W., Shim, Y.H., Bang, J.S., Oh, J.S., Wan, K.S., Seo, M.H., 2001. In vivo
evaluation of polymeric micellar paclitaxel formulation: toxicity and efficacy. J.
Control. Release 72, 191–202.
Acknowledgements
Li, H., Qian, Z.M., 2002. Transferrin/transferrin receptor-mediated drug delivery.
Med. Res. Rev. 22, 225–250.
This research was supported by Basic Science Research Pro-
gram through the National Research Foundation of Korea (NRF)
funded the Ministry of Education, Science and Technology (2011-
Liang, N., Sun, S., Li, X., Piao, H., Piao, H., Cui, F., Fang, L., 2012. a-Tocopherol succinate-
modified chitosan as a micellar delivery system for paclitaxel: preparation,
characterization and in vitro/in vivo evaluations. Int. J. Pharm. 423, 480–488.
Liu, X.F., Guan, Y.L., Yang, D.Z., Li, Z., Yao, K.D., 2001. Antibacterial action of chitosan
and carboxymethylated chitosan. J. Appl. Polym. Sci. 79, 1324–1335.
Liu, Z., Jiao, Y., Wang, Y., Zhou, C., Zhang, Z., 2008. Polysaccharides-based nanopar-
ticles as drug delivery systems. Adv. Drug Deliv. Rev. 60, 1650–1662.
Nah, J.W., Jang, M.K., 2002. Spectroscopic characterization and preparation of low
molecular water soluble chitosan with free-amine group by novel method. J.
Polym. Sci. Part A: Polym. Chem. 40, 3796–3803.
Nam, J.P., Kim, D.G., Kim, Y.B., Jeong, Y.I., Jang, M.K., Nah, J.W., 2008. Prepara-
tion and NMR spectroscopic characterization of low molecular water soluble
O-carboxymethyl chitosan. J. Chitin Chitosan. 13, 105–109.
Perales, J.C., Ferkol, T., Molas, M., Hanson, R.W., 1994. An evaluation of receptor-
mediated gene transfer using synthetic DNA–ligand complexes. Eur. J. Biochem.
0012039).
References
Allen, C., Dos Santos, N., Gallagher, R., Chiu, G.N., Shu, Y., Li, W.M., Johnstone, S.A.,
Janoff, A.S., Mayer, L.D., Webb, M.S., Bally, M.B., 2002. Controlling the physical
behavior and biological performance of liposome formulations through use of
surface grafted poly(ethylene glycol). Biosci. Rep. 22, 225–250.
Anabousi, S., Bakowsky, U., Schneider, M., Huwer, H., Lehr, C.M., Ehrhardt, C., 2006.
In vitro assessment of transferrin-conjugated liposomes as drug delivery sys-
tems for inhalation therapy of lung cancer. Eur. J. Pharm. Sci. 29, 367–374.
Brocchini, S., Duncan, R., 1999. Encyclopaedia of Controlled Drug Delivery. Wiley,
New York, pp. 786–816.
226, 255–266.
Qian, Z.M., Li, H., Sun, H., Ho, K., 2002. Targeted drug delivery via the transferrin
receptor-mediated endocytosis pathway. Pharmacol. Rev. 54, 561–587.
Qu, G., Yao, Z., Zhang, C., Wu, X., Ping, Q., 2009. PEG conjugated N-octyl-O-sulfate
chitosan micelles for delivery of paclitaxel: in vitro characterization and in vivo
evaluation. Eur. J. Pharm. Sci. 37, 98–105.
Burt, H.M., Zhang, X., Toleikis, P., Embree, L., Hunter, W.L., 1999. Development of
copolymers of poly(d,l-lactide) and methoxypolyethylene glycol as micellar
carriers of paclitaxel. Colloids Surf. B: Biointerfaces 16, 161–171.
Chang, J., Jallouli, Y., Kroubi, M., Yuan, X.B., Feng, W., Kang, C.S., Pu, P.Y., Betbeder, D.,
2
009. Characterization of encocytosis of transferrin-coated PLGA nanoparticles
Richardson, D.R., Ponka, P., 1997. The molecular mechanisms of the metabolism and
transport of iron in normal and neoplastic cells. Biochim. Biophys. Acta 1333,
by the blood–brain barrier. Int. J. Pharm. 379, 285–292.
Chen, L.Y., Du, Y.M., Tian, Z.G., Sun, L.P., 2005. Effect of the degree of deacetylation
and the substitution of carboxymethyl chitosan on its aggregation behavior. J.
Polym. Sci. Part B: Polym. Phys. 43, 296–305.
Chen, X.G., Park, H.J., 2003. Chemical characteristics of O-carboxymethyl chitosans
related to the preparation conditions. Carbohydr. Polym. 53, 355–359.
Cho, K., Wang, X., Nie, S., Chen, Z.G., Shin, D.M., 2008. Therapeutic nanoparticles for
drug delivery in cancer. Clin. Cancer Res. 14, 1310–1316.
Dufes, C., Muller, J.M., Couet, W., Olivier, J.C., Uchegbu, I.F., Schatzlein, A.G., 2004.
Anticancer drug delivery with transferrin targeted polymeric chitosan vesicles.
Pharm. Res. 21, 101–107.
Elbert, D.L., Hubbell, J.A., 1996. Surface treatments of polymers for biocompatibility.
Ann. Rev. Mater. Sci. 26, 365–394.
Fu, Q., Sun, J., Ai, X., Zhang, P., Li, M., Wang, Y., Liu, X., Sun, Y., Sui, X., Sun, L., Han,
X., Zhu, M., Zhang, Y., Wang, S., He, Z., 2013. Nimodipine nanocrystals for oral
bioavailability improvement: role of mesenteric lymph transport in the oral
absorption. Int. J. Pharm. 448, 290–297.
Hong, M., Zhu, S., Jiang, Y., Tang, G., Sun, C., Fang, C., Shi, B., Pei, Y., 2010. Novel
anti-tumor strategy: Peg-hydroxycamptothecin conjugate loaded transferrin-
PEG-nanoparticles. J. Control. Release 14, 22–29.
1–40.
Ruiz, L.C., Fuente, M.D.L., Parraga, J.E., Garcia, A.L., Fernandez, I., Seijo, B., Sanchez,
A., Calonge, M., Diebold, Y., 2011. Intracellular trafficking of hyaluronic acid-
chitosan oligomer based nanoparticles in cultured human ocular surface cells.
Mol. Vis. 17, 279–290.
Sahu, S.K., Maiti, S., Maiti, T.K., Ghosh, S.K., Pramanik, P., 2011a. Folate decorated
succinyl chitosan nanoparticles conjugated with doxorubicin for targeted drug
delivery. Macromol. Biosci. 11, 285–295.
Sahu, S.K., Mallick, S., Santra, S., Maiti, T.K., Ghosh, S.K., Pramanik, P., 2010. In vitro
evaluation of folic acid modified carboxymethyl chitosan nanoparticles loaded
with doxorubicin for targeted delivery. J. Mater. Sci.: Mater. Med. 21, 1578–1588.
Sahu, S.K., Maiti, S., Maiti, T.K., Ghosh, S.K., Pramanik, P., 2011b. Hydrophobically
modified carboxymethyl chitosan nanoparticles targeted delivery of paclitaxel.
J. Drug Target. 19, 104–113.
Sahu, S.K., Maiti, S., Pramanik, A., Ghosh, S.K., Pramanik, P., 2012. Controlling the
thickness of polymeric shell on magnetic nanoparticles loaded with doxorubicin
for targeted delivery and MRI contrast agent. Carbohydr. Polym. 87, 2593–2604.
Senior, J.H., 1987. Fate and behavior of liposomes in vivo: a review of controlling
factors. Crit. Rev. Ther. Drug Carrier Syst. 3, 123–193.
Huebers, H.A., Finch, C.A., 1987. The physiology of transferrin and transferrin recep-
tors. Physiol. Rev. 67, 520–582.
Singla, A.K., Garg, A., Aggarwal, D., 2002. Paclitaxel and its formulations. Int. J. Pharm.
235, 179–192.
Huennekens, F.M., 1994. Tumor targeting: activation of prodrugs by
enzyme–monoclonal antibody conjugates. Trends Biotechnol. 12, 234–239.
Kainthan, R.K., Gnanamani, M., Ganguli, M., Ghosh, T., Brooks, D.E., Maiti,
S., Kizhakkedathu, J.N., 2006. Blood compatibility of novel water soluble
hyperbranched polyglycerol-based multivalent cationic polymers and their
interaction with DNA. Biomaterials 27, 5377–5390.
Tomasina, J., Lheureux, S., Gauduchon, P., Rault, S., Freon, A.M., 2013. Nanocarriers
for the targeted treatment of ovarian cancers. Biomaterials 34, 1073–1101.
Vaidya, B., Vyas, S.P., 2012. Transferrin coupled vesicular system for intracellular
drug delivery for the treatment of cancer: development and characterization. J.
Drug Target. 20, 372–380.